Qualitätssicherung im andrologischen Labor

Ochsendorf, Falk; Beschmann, Heike A.

doi:10.1007/s004440050025

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…At present, EQC for the determination of sperm concentration, motility, and morphology is being widely appreciated in Europe (Neuwinger et al ., ; Cooper, ; Jørgensen et al ., ; Ochsendorf & Beschmann, ; Auger et al ., ; Alvarez et al ., ; Mallidis et al ., ; Jedrzejczak et al ., ; Filimberti et al ., ) and other parts of the world (Keel et al ., ; Brazil et al ., ; Zuvela et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Fifteen years of Belgian experience with external quality assessment of semen analysis

et al. 2016

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Semen analysis is difficult to standardize, quality control and quality assurance are necessary to ensure that results are accurate and precise. This Belgian EQA survey over a 15-year period, involving 121 laboratories, attempted to reduce interlaboratory variability and at the same time, encouraged participating laboratories to implement correct techniques as advised by the WHO. Over the total period, the median coefficient of variation (CV) for sperm count, irrespective of the method used was 19.2%, while using improved Neubauer chamber resulted in a significantly (p < 0.001) lower median CV (14.4%). The overall median CV for rapid progressive motility was high (37.1%), but progressive motility (15.1%) and total motility (13.8%) were acceptable. Sperm morphology revealed a large variability in 79.4% irrespective of the staining procedures or evaluation criteria used. Participation in the Belgian EQA is on voluntary basis. Both, participation and implementation of the correct techniques should be made mandatory for accreditation and benefit of patient treatment. The existing Belgian EQA program should now be harmonized with other existing EQA schemes in Europe.

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Section: Introductionmentioning

confidence: 99%

Fifteen years of Belgian experience with external quality assessment of semen analysis

et al. 2016

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“…The necessity for external quality control (EQC) for semen analysis is being appreciated in Europe (Cooper, 1996; Jørgensen et al ., 1997; Ochsendorf & Beschmann, 1998; Auger et al ., 2000) and further afield (WHO, 1999). Currently several schemes are being conducted in Europe in which technicians in participating laboratories assess aliquots of the same semen sample and receive notification of how well they agree with the values designated by the EQC programme (trimmed mean values or consensus values from expert laboratories).…”

Section: Introductionmentioning

confidence: 99%

Semen analysis and external quality control schemes for semen analysis need global standardization

Cooper¹,

Björndahl

Vreeburg

et al. 2002

Int J of Andrology

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One semen analysis laboratory [the Institute of Reproductive Medicine (IRM), Münster, Germany] was enrolled in three external quality assurance programmes in Europe (United Kingdom External Quality Assurance Scheme, European Academy of Andrology, European Society of Human Reproduction) that control for the assessment of sperm concentration, sperm motility and sperm morphology. Agreement between the participating laboratory and the sperm concentrations designated by all three programmes was good. Disagreement between two quality control (QC) programmes providing motility assessment was particularly noticeable in the distinction between motility grades a and b. For the identification of normal sperm morphology, marked differences between the standards set by different QC programmes were apparent. One programme was stricter in its categorization of normal forms, such that an overestimation of normal forms was diagnosed at IRM, whereas agreement with the other programmes was achieved. Variation of results from technicians in the IRM internal QC programme was <13%. The discrepancies between external quality control (EQC) centres demonstrated here are challenging problems to be overcome partly by the andrology laboratories and partly by the providers of EQC services. The introduction of objective, computer-aided sperm assessment in providing designated values may help to rectify this situation. Until this is achieved, EQC programmes should develop an internal programme to monitor their materials and methods for assessment.

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