Qualitative ultrastructural changes and morphometry of deccani sheep spermatozoa preserved with egg yolk citrate extender

Rajashri, M.; Reddy, K Ramchandra; Kumari, G. Aruna; Kumari, N. Nalini; Kesharwani, Surabhi; Gurram, Srinivas

doi:10.31018/jans.v9i3.1394

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…A circular tail characterized sperm cells with intact membranes at the end. Meanwhile, those with incomplete membranes were characterized by straight-tail conditions (Rajashri et al, 2017).…”

Section: Observation Of Semen Deteriorationmentioning

confidence: 99%

Deterioration of Frozen Semen of Bali Cattle after Cooling at 5°C

Tethool¹,

Ciptadi²,

Wahjuningsih³

et al. 2022

WVJ

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Frozen semen is produced through several stages, which deteriorate spermatozoa. This research aimed to evaluate the deterioration degree of frozen semen after 5 °C cooling and freezing of Bali cattle. The samples included 10 male Bali cattle with a body weight of 542-668 kg, from which semen was collected once a week for five weeks. The deterioration of each individual’s sperm was determined by observing two distinct straws. The parameters observed included viability, abnormalities, intact plasma membrane, and intact acrosome cap. Initial observations of the parameters were conducted following the addition of semen to diluent A1 (AD) as much as the volume of fresh semen. The semen in the AD group was not cooled and frozen. The A1 semen was then divided into two, namely, those with cooling at 5 °C for 4 hours (PT1) and at 5°C for 22 hours (PT2). The results showed that individual variations in Bali cattle caused significant differences in viability and intact plasma membrane of AD and PT1 groups, while PT2 did not differ in viability and intact plasma membrane spermatozoa. Abnormalities were significantly different between AD and PT2 groups, however PT1 did not differ in abnormalities spermatozoa. Intact acrosomal cap was significantly different in the AD, PT1, and PT2 groups. In conclusion, individual variations, including viability, abnormalities, intact plasma membrane, and acrosome cap of spermatozoa, were better at 4 hours compared to cooling at 5°C for 22 hours. A Cooling time of 4 hours at 5°C can be recommended for frozen semen processing.

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Section: Observation Of Semen Deteriorationmentioning

confidence: 99%

Deterioration of Frozen Semen of Bali Cattle after Cooling at 5°C

Tethool¹,

Ciptadi²,

Wahjuningsih³

et al. 2022

WVJ

View full text Add to dashboard Cite

show abstract

“…Scanning electron microscopy (SEM) provides three‐dimensional micrographs with details of cell surface and depth (McClusky, ). It was a useful tool for the evaluation of the effects derived from the temperature reduction during refrigeration or cryopreservation of the sperm of goats (Chauhan, Kapila, Gandhi, & Anand, ), rams (Rajashri et al, ), and boars (Ringleb, Waurich, Wibbelt, Streich & Jewgenow, ). In addition, transmission electronic microscopy (TEM) techniques provides two‐dimensional images, allowing the observation of the interior of the cells with a wealth of detail.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural description of fresh and frozen/thawed sperm derived from collared peccaries (Pecari tajacu Linnaeus, 1,758)

Bezerra

Souza

Silva

et al. 2018

Microscopy Res & Technique

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The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen-thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen-thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM. Sperm motility, membrane integrity, and functionality were impaired (p < .05) by freezing-thawing procedures, but sperm morphology, and chromatin integrity evaluated by light microscopy were not significantly affected. The SEM revealed that peccaries' sperm presents a flattened head in a paddle format, measuring 6.07 μm in length and 3.84 μm in width, with a vastus acrosome (4.46 μm). Normal tails measure 38.11 μm, being formed by an extensive midpiece with 15.52 μm in length. In frozen-thawed samples, both SEM and TEM provide us information about damage undetected through light microscopy as the presence of vesicles in the acrosome, loose plasma membrane, vacuolized mitochondria, dense fibers disorganized, and decondensed chromatin. In conclusion, we provide the first description of the sperm ultrasctruture in collared peccaries. Moreover, SEM and TEM help us to identify some nanometric damage provoked by freezing-thawing procedures, thus providing valuable information for the improvement of such important protocols used for biobanking formation. K E Y W O R D Sfreezing-thawing, morphology, scanning electron microscopy, semen, Tayassu tajacu, transmission electron microscopy

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Qualitative ultrastructural changes and morphometry of deccani sheep spermatozoa preserved with egg yolk citrate extender

Cited by 2 publications

References 24 publications

Deterioration of Frozen Semen of Bali Cattle after Cooling at 5°C

Deterioration of Frozen Semen of Bali Cattle after Cooling at 5°C

Ultrastructural description of fresh and frozen/thawed sperm derived from collared peccaries (Pecari tajacu Linnaeus, 1,758)

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