Qualitative Time-of-Flight Secondary Ion Mass Spectrometry Analysis of Root Dentin Irrigated with Sodium Hypochlorite, EDTA, or Chlorhexidine

Kolosowski, Kamil P.; Sodhi, Rana N.S.; Kishen, Anil; Basrani, Bettina

doi:10.1016/j.joen.2015.06.010

Cited by 9 publications

(6 citation statements)

References 33 publications

(69 reference statements)

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“…Structure's changes can also result from the changes of the number of mineral components of the radicular dentine and the depth of penetration of irrigants used (3). Dentine has an intense collagen network coated by hydroxyapatite (18,19).…”

Section: Resultsmentioning

confidence: 99%

A comparative ex-vivo study of effects of different irrigation protocols with/without laser activation on the root dentine’s micro-hardness

et al. 2019

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To compare the effects of different irrigation protocols, with/without laser activation, on the radicular dentine's micro-hardness. Methods: Eighty-two human extracted premolars were decoronated and divided into 7 groups. Roots were longitudinally split into two halves. The micro-hardness was measured for one half before and after irrigation protocols. The groups were; G1: MTAD without laser-activation, G2: MTAD with laser-activation, G3: sodiumhypochlorite (SH) with laser-activation, G4: SH then EDTA with laser-activation, G5: SH then MTAD with laseractivation, G6: SH without laser-activation. G7: distilled water (control). In the two-irrigants groups G4 and G5), samples were irrigated first with SH then with MTAD or EDTA irrigants, which were activated by the laser. The difference between the before-and after-irrigation micro-hardness was calculated to obtain the microhardness difference. Data were analyzed using the Paired Sample-t and Two-ways ANOVA tests at P=0.05. Results: Overall, the mean dentine's micro-hardness after-irrigation (103.1) was lower than before-irrigation (116.1) (P<0.001); except for the distilled-water group, (116.6 and 112.9, respectively) (P=0.075). The microhardness reduction of SH without laser-activation group (32.5) was the greatest (P<0.001). The single-irrigant or laser-activation irrigation protocols caused significantly less micro-hardness reduction compared to the two-irrigants or no laser-activation protocols. The mean micro-hardness reduction of SH and MTAD groups (both with laser-activation) (5.8 and 9.3, respectively) were significantly lower than other groups, but not from that of the control group (3.7). Conclusion:Using irrigants significantly reduced the root-dentine's micro-hardness. Although irrigants agitation by an Er: Yag laser significantly minimized micro-hardness reduction, it did not suppress the adverse effects on dentine micro-hardness when two-irrigants were used.

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Section: Resultsmentioning

confidence: 99%

A comparative ex-vivo study of effects of different irrigation protocols with/without laser activation on the root dentine’s micro-hardness

et al. 2019

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“…Similarly, deproteinization of human bone samples led to a decrease in carbonate content, as a consequence of exposure to NaClO. Additionally, Nielsen‐Marsh and Hedges () and Kolosowski, Sodhi, Kishen, and Basrani () showed that both procedures (acetic acid and NaClO wash) lead to different bioapatite compositions. Therefore, we chose not to pre‐treat our samples, even though they were subjected to an undisclosed but short‐term burial.…”

Section: Discussionmentioning

confidence: 95%

Crystal clear: Vibrational spectroscopy reveals intrabone, intraskeleton, and interskeleton variation in human bones

Gonçalves

Vassalo

Mamede

et al. 2018

American J Phys Anthropol

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The relatively large heterogeneity, especially at the intrabone level, is possibly the consequence of microstructural bone differences. The dissimilarities observed between our investigation and other published studies are probably due to the fact that the samples used here came from human rather than faunal bones. Also, our samples were buried previously to the experimental burning so this may also partly explain our contrasting results, since previous research was mostly performed on fresh bone. Future inferences based on vibrational spectroscopy analyses should take into account the possible effect of all these sources.

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“…Regarding lipids removal, chloroform/methanol and petroleum ether solutions are the most commonly used(43,(96)(97)(98), although it takes several hours, no negative effects on the mineral lattice having been reported. Concerning to deproteinization, the numerous suggested protocols -using ethylenediamine(99,100), sodium hypochlorite(100,101) or hydrazine(102), among others -were found to affect the inorganic matrix of bone(100,103,104), which requires the development of improved methods.• Bleaching -either photobleaching -photochemical destruction of the fluorophore(s) by prolonged exposure of the sample to the laser beam (80) -or chemical bleaching -removal or significant damage of the fluorophore by treatment with organic solvents. Although chemical bleaching effectively reduces fluorescence, it may also damage the sample (93, 105).…”

mentioning

confidence: 99%

Burned bones tell their own stories: A review of methodological approaches to assess heat-induced diagenesis

Mamede

Gonçalves

Marques

et al. 2017

Applied Spectroscopy Reviews

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One of the biggest struggles of biological anthropology is to estimate the biological profile from burned human skeletal remains. Bioanthropological methods are seriously compromised due to bone heat-induced alterations in shape and size. Therefore, it is urgent to improve our ability to estimate sex, age at death, stature and ancestrality, to recognize peri mortem traumas and differentiate them from fractures due to fire, and to determine what was the intensity of burning, namely maximum temperature and heat exposure length. This review focuses on different methodologies to assess heat prompted changes in bone submicrostructure. Some of these are extensively used in burned bones research, namely infrared and Raman spectroscopy and X-ray diffraction, while others such as neutron spectroscopy and diffraction are rarely applied to bone samples although their contribution may be crucial for establishing new bioanthropological methods for a reliable examination of burned victims.

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Qualitative Time-of-Flight Secondary Ion Mass Spectrometry Analysis of Root Dentin Irrigated with Sodium Hypochlorite, EDTA, or Chlorhexidine

Cited by 9 publications

References 33 publications

A comparative ex-vivo study of effects of different irrigation protocols with/without laser activation on the root dentine’s micro-hardness

A comparative ex-vivo study of effects of different irrigation protocols with/without laser activation on the root dentine’s micro-hardness

Crystal clear: Vibrational spectroscopy reveals intrabone, intraskeleton, and interskeleton variation in human bones

Burned bones tell their own stories: A review of methodological approaches to assess heat-induced diagenesis

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