“…Although neglected by analytical chemists, during the same period supercritical fluid extraction gained acceptance and found many applications as an industrial scale process technique, used for example, to decaffeinate coffee and to extract nicotine from tobacco [98,246]. In contemporary practice, however, the main use has been in combined techniques in which supercritical fluid extraction using a micro-extractor is coupled on-line to gas, liquid, thin-layer or supercritical fluid chromatography [223,[248][249][250][251][252][253][254][255][256][257]. In contemporary practice, however, the main use has been in combined techniques in which supercritical fluid extraction using a micro-extractor is coupled on-line to gas, liquid, thin-layer or supercritical fluid chromatography [223,[248][249][250][251][252][253][254][255][256][257].…”