Qualitative expression of hypoxia-inducible factor-1α in malignant transformation of oral submucous fibrosis: An immunohistochemical study

Pereira, Treville; Surve, Ridima; Shetty, Subraj; Gotmare, Swati

doi:10.4103/jomfp.jomfp_234_19

Cited by 11 publications

(6 citation statements)

References 27 publications

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“…However the comparison of our study with study by Hande and coworkers had certain limitations like the difference in sample used (saliva vs. tissue) and analytical techniques employed in the studies (ELISA vs. immunohistochemistry). Pereira, Dalmia, Chaudhry and Tilakaratne also evaluated HIF-1α expression in OSMF and controls and showed statistically significant results [17][18][19][20] . Again, these researches involved tissue biopsy, immunohistochemistry and polymerase chain reaction (PCR) for biomarker identification.…”

Section: Discussionmentioning

confidence: 99%

Salivary Expression of HIF-1 α in Oral Submucous Fibrosis

2021

PJMD

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Background: Oral submucous fibrosis (OSMF), a chronic debilitating condition distinguished by juxtaepithelial fibrosis or extensive fibrosis of submucosa and reduced vascularity, results in compromised blood supply causing tissue hypoxia. This brings about the transcription of a set of genes associated with angiogenesis, breakdown of iron/glucose, cell division and cell stability. Hypoxia Inducible Factor-1α (HIF-1α) is the main biomarker intervening in this reaction. The aim of research was to measure the salivary levels of HIF-1α in OSMF patients and healthy controls.Methods: This study included 60 participants (30 Oral submucous fibrosis cases and 30 healthy controls). The consecutive sampling technique was used for the recruitment of the study participants. The enzyme-linked immunosorbent assay (ELISA) was used to evaluate HIF-1α levels in saliva. In order to determine data normality, the Kolmogorov-Smirnov test was used. Independent t test was applied to compare salivary levels of HIF-1α between cases and controls and p-value of less than 0.05 was considered statistically significant. Results:The mean salivary HIF-1α value for the oral submucous fibrosis group was 20.18±7.78, compared to healthy controls 13.62±8.86. A statistically significant difference was seen between levels of HIF-1α in the OSMF and control group (p=0.003). The mean mouth opening for cases was 22.94±9.51 mm and for controls 42.00±5.19 mm. There was no correlation among salivary levels of HIF-1α in both the case and control groups (-0.059, -0.030) respectively. Conclusion:Higher levels of HIF-1α were seen in the OSMF group in comparison to healthy individuals suggesting that HIF-1α may play a role in malignant transformation of OSMF.

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Section: Discussionmentioning

confidence: 99%

Salivary Expression of HIF-1 α in Oral Submucous Fibrosis

2021

PJMD

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“…HIF-1α degradation is inhibited due to hypoxic conditions in addition to some other conditions including normoxic conditions [34]. HIF-1α activates the downstream target genes transcription which regulate many biological significant processes, including angiogenesis, glucose metabolism, cell proliferation, pH regulation, and migration [35,36]. Thus, oral premalignant disorders like oral submucous fibrosis (OSMF) were also found to have a significant HIF-1α expression in the basal and suprabasal layers of the epithelium, according to Pereira TV et al (2020) which demonstrates the role of hypoxia in OSMF malignant transformation and that the HIF-1α expression is an early event in oral carcinogenesis [36].…”

Section: Immunohistochemical Findingsmentioning

confidence: 99%

Untitled

2024

J. Med. Chem. Sci.

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This study aimed to evaluate the expression of hypoxia inducible factor-1 alpha (HIF-1α) and CD44 markers to detect their potential role in the progression of oral epithelial dysplasia (OED) to oral squamous cell carcinoma (OSCC). The study involved 50 patients with different grades of OED and OSCC taken from different anatomical sites by the head and neck surgeons. The pathologists used ordinary stained slides for histological evaluation and immunostaining of HIF-1α and CD44 antibodies using conventional and remote examination tools. The results showed that the mean area percentage of HIF-1α and CD44 immunostaining was significantly higher in severe dysplasia, followed by moderate dysplasia and mild dysplasia. Similar results were observed in OSCC, with poorly differentiated OSCC showing the highest mean expression and the mean area percentages in all cases were higher in OSCC (44.24%) and (47.09%) than in OED (14.50%) and (20.29%). The study concluded that HIF-1α and CD44 markers could be helpful in accurate classification of oral epithelial lesions with potential and therapeutic value, however further clinical studies are recommended. K E Y W O R D S HIF-1α CD44Oral squamous cell carcinoma Oral epithelial dysplasia Immunohistochemistry Telepathology

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“…In solid tumors like OSCC, the malignant cells proliferate exponentially which causes an imbalance in the oxygen supply due to poor vascular architecture of the neo-angiogenic vessels. This insufficient oxygen concentration (pO 2 < 10 mmHg) in neoplastic cells results in hypoxia as it exceeds the required angiogenic demand [ 4 , 5 ]. Hypoxia is the most common characteristic of solid tumors enabling them to adapt and survive in TME with reduced oxygen concentration as severe hypoxia causes cell death in normal conditions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Study of Expression of Hypoxia-Inducible Factor-1 Alpha (HIF-1 Alpha) and Hypoxia-Inducible Factor-2 Alpha (HIF-2 Alpha) in Oral Squamous Cell Carcinoma: An Immunohistochemical Study

Mahapatra,

Panda,

Dash

et al. 2023

Cureus

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Context: Oral cancer is the major cause of mortality and morbidity worldwide. There are many factors that influence the tumor microenvironment that promotes tumorigenesis. Hypoxia is one of the factors that affects the process of angiogenesis by inducing proangiogenic factors to maintain the blood supply which in turn enhances the aggressiveness of the tumor and prognosis of solid tumors such as oral squamous cell carcinoma. Aim and objective: The aim of the study was to compare the expression of hypoxia-inducible factor 1α (HIF-1α) and hypoxia-inducible factor 2α (HIF-2α) in various histological grades of oral squamous cell carcinoma immunohistochemically. Methodology: Immunohistochemical evaluation of HIF-1α and HIF-2α was done in 90 samples of oral squamous cell carcinoma which were graded histologically into 30 samples each of well, moderately and poorly differentiated squamous cell carcinoma. Statistical evaluation: Statistical analysis was done to study the prognostic significance of the biomarkers. Results: All the cases showed positivity for expression of HIF-1α and HIF-2α. The number of positive staining in both markers reduced as the tumor severity increased from well to poorly differentiated. The expression of MIL of HIF-2α was higher than HIF 1α and HIF 2α expression was mostly seen in cytoplasmic in well-differentiated and nuclear in both moderately and poorly differentiated OSCC suggestive that HIF-2α is a more specific marker to hypoxia. Conclusion: Hypoxia is an essential factor that triggers other angiogenic switch and inflammatory factors which facilitates the process of tumorigenesis. This is also important for predicting the treatment outcome and prognosis of the patients. HIF-2α is a more sensitive marker that appears to be correlated and could perhaps serve as a good surrogate marker of hypoxia.

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Qualitative expression of hypoxia-inducible factor-1α in malignant transformation of oral submucous fibrosis: An immunohistochemical study

Cited by 11 publications

References 27 publications

Salivary Expression of HIF-1 α in Oral Submucous Fibrosis

Salivary Expression of HIF-1 α in Oral Submucous Fibrosis

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The Study of Expression of Hypoxia-Inducible Factor-1 Alpha (HIF-1 Alpha) and Hypoxia-Inducible Factor-2 Alpha (HIF-2 Alpha) in Oral Squamous Cell Carcinoma: An Immunohistochemical Study

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