Qualitative evidence of anti-Yo-specific intrathecal antibody synthesis in patients with paraneoplastic cerebellar degeneration

Qualitative evidence of Ri specific IgG-synthesis in the cerebrospinal fluid from patients with paraneoplastic neurological syndromes

“…This argues in favour of a higher sensitivity of anti-Ri specific affinity blotting compared to conventional affinity blotting with anti-human IgG. Our findings are in accordance with a previous study suggesting a higher sensitivity of antigen-specific blotting in patients with anti-Yo syndrome [12]. These results could be of interest in detection of intrathecal specific IgG synthesis in other antibody-associated diseases of the CNS, including those of infectious origin.…”

“…We can therefore not fully exclude that CNS symptoms in patients with PNS may rarely occur independent of intrathecal IgG production against the respective antigen. This hypothesis would be in accordance with reports favouring a T cell mediated rather than an antibody-mediated mechanism of damage in PNS [1] and differs from our previous assumptions on a stringent association between the presence of specific intrathecal antibody synthesis in PNS patients with CNS involvement [11,12]. More likely, however, the more restricted site of inflammation in limbic encephalitis as well as the fact that brain IgG contributes relatively little to lumbar CSF may account for that finding.…”

“…In previous studies, we provided qualitative evidence of HuD-and Yo-specific intrathecal antibody synthesis by demonstration of specific oligoclonal bands in CSF from patients with known anti-Hu or anti-Yo syndrome [11,12]. Using this approach, we now investigated five serum/CSF pairs from patients with anti-Ri syndrome for the presence of Ri-specific oligoclonal bands.…”

“…However, the oligoclonal IgG bands have not been not demonstrated to be specific for Ri antigen so far. In two previous studies [11][12][13], anti-HuDspecific and anti-Yo-specific oligoclonal bands in CSF, revealing qualitative evidence of anti-HuD-specific and antiYo-specific intrathecal IgG synthesis were reported. To confirm and extend our finding from a single case study suggesting the presence of specific IgG bands also in a patient with anti-Ri syndrome [13], we performed a combination of isoelectric focusing and a highly sensitive affinity immunoblot in five patients with anti-Ri syndrome.…”

CSF proteome: a protein repository for potential biomarker identification

“…Identification of autoantibodies, anti-Yo and anti-Hu, in the CSF are diagnostic for certain breast, lung and ovarian tumors [51,52]. Detection of these antibodies in the CSF, in the absence of overt malignant disease, indicates a high likelihood of an occult tumor.…”