Qualitative drug analysis of hair extracts by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry

Guthery, Bill; Bassindale, Thomas; Bassindale, Alan R.; Pillinger, C. T.; Morgan, G. H.

doi:10.1016/j.chroma.2010.04.020

Cited by 55 publications

(27 citation statements)

References 16 publications

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“…Hoh et al also used this approach to develop a method that allowed for the analysis of a wide range of environmental contaminants in fish oil [41]. Other reports of broader screening during targeted analysis were also found in the literature [38,42].…”

Section: Targeted Analysismentioning

confidence: 94%

Qualitative and quantitative approaches in comprehensive two-dimensional gas chromatography

Murray

2012

Journal of Chromatography A

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Section: Targeted Analysismentioning

confidence: 94%

Qualitative and quantitative approaches in comprehensive two-dimensional gas chromatography

Murray

2012

Journal of Chromatography A

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“…The method required no prior derivatization of the components, which launched the main advantage regarding most of the published methods [17][18][19][20][21][22]. The chromatographic conditions, such as injector temperature (190,210,230,250,270, and 290°C), initial column temperature (100, 150, 180, 200, and 220°C), final column temperature (290, 300, and 310°C), initial temperature hold time (0.5, 3, and 5.5 min), column temperature rate (20, 30, 40°C/min), and carrier gas flow rate (1.0, 1.5, и 2.0 ml/min) were all optimized.…”

Section: Resultsmentioning

confidence: 99%

“…Gas chromatography (GC) is the method of choice for bioanalysis of opium alkaloids [14][15][16][17][18][19][20][21][22], but for the analysis of the plant extracts may be less convenient due to the many less volatile substances co-extracted [9]. The major constrain of the GC methods is the commonly used prior derivatization of the analytes in order to volatilize them, avoid broadening of the peaks in order to achieve easier quantification.…”

mentioning

confidence: 99%

Chemometric approach for development, optimization, and validation of different chromatographic methods for separation of opium alkaloids

et al. 2012

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The excessive and continuously growing interest in the simultaneous determination of poppy alkaloids imposes the development and optimization of convenient high-throughput methods for the assessment of the qualitative and quantitative profile of alkaloids in poppy straw. Systematic optimization of two chromatographic methods (gas chromatography (GC)/flame ionization detector (FID)/mass spectrometry (MS) and reversed-phase (RP)-high-performance liquid chromatography (HPLC)/diode array detector (DAD)) for the separation of alkaloids from Papaver somniferum L. (Papaveraceae) was carried out. The effects of various conditions on the predefined chromatographic descriptors were investigated using chemometrics. A full factorial linear design of experiments for determining the relationship between chromatographic conditions and the retention behavior of the analytes was used. Central composite circumscribed design was utilized for the final method optimization. By conducting the optimization of the methods in very rational manner, a great deal of excessive and unproductive laboratory research work was avoided. The developed chromatographic methods were validated and compared in line with the resolving power, sensitivity, accuracy, speed, cost, ecological aspects, and compatibility with the poppy straw extraction procedure. The separation of the opium alkaloids using the GC/FID/MS method was achieved within 10 min, avoiding any derivatization step. This method has a stronger resolving power, shorter analysis time, better cost/effectiveness factor than the RP-HPLC/DAD method and is in line with the "green trend" of the analysis. The RP-HPLC/DAD method on the other hand displayed better sensitivity for all tested alkaloids. The proposed methods provide both fast screening and an accurate content assessment of the six alkaloids in the poppy samples obtained from the selection program of Papaver strains.

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“…El carácter no específico, de amplio espectro, del análisis GC × GC, se ilustra en el trabajo publicado por Guthery et al [31] , sobre análisis de drogas en muestras de cabello por técnicas tió el desarrollo de este método para el análisis simultáneo de 5 aminas relacionadas estructuralmente, cuyos tiempos de retención son cercanos.…”

Section: La Cromatografía De Gases Multidimensional Y La Perfilaciónunclassified

GC-MS: herramienta fundamental para el análisis de drogas de uso ilícito

Stashenko¹,

Martı́nez²

2012

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ResumoLa determinación cromatográfica de drogas de uso ilícito presenta retos importantes en varias etapas de la cadena analítica debido a la volatilidad relativamente baja de los analitos, a la diversidad grande de los posibles contaminantes y a las concentraciones bajas en las que se encuentran algunas sustancias de interés. La preparación de la muestra, el uso de agentes de derivación, los modos de operación del espectrómetro de masas para la detección y los diversos campos de aplicación del análisis GC-MS de sustancias controladas se discuten en este artículo junto con la presentación de algunos ejemplos recientes de estas aplicaciones. Palavras-chaveCromatografía de gases; cromatografía de gases acoplada a espectrometría de masas; sustancias controladas; drogas. GC-MS: fundamental tool for the analysis of drugs of illicit use AbstractThe chromatographic determination of illicit drugs poses important challenges at various stages along the analytical procedure, due to the low volatility of the analytes, the diversity of potential contaminants, and to the low concentrations of certain substances of interest. Sample preparation, the use of derivatization agents, different operating modes of the mass spectrometer for the detection, and the diverse application fields of the GC-MS analysis of illicit drugs are discussed in this article together with the presentation of some examples of these applications.

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Qualitative drug analysis of hair extracts by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry

Cited by 55 publications

References 16 publications

Qualitative and quantitative approaches in comprehensive two-dimensional gas chromatography

Qualitative and quantitative approaches in comprehensive two-dimensional gas chromatography

Chemometric approach for development, optimization, and validation of different chromatographic methods for separation of opium alkaloids

GC-MS: herramienta fundamental para el análisis de drogas de uso ilícito

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