Qualitative detection of enterovirus D68 from PrimeStore® molecular transport medium: implications for home- and self-collection

Nguyen-Tran, Hai; Reno, Samantha; Mwangi, Eric; Mentel, Marek; Hengartner, Randy; Dominguez, Samuel R.; Messacar, Kevin; Jung, Sarah

doi:10.1016/j.diagmicrobio.2023.115976

Cited by 1 publication

(1 citation statement)

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“…Midturbinate swab samples were stored in PrimeStore Molecular Transport Media (Longhorn Vaccines and Diagnostics, https://www.lhnvd.com ), which was previously validated by our laboratory for home collection and storage conditions for EV-D68 qualitative detection ( 21 ). Samples were stored in a refrigerator, delivered from home after the 21-day collection period, then aliquoted and frozen at −80°C until testing.…”

Section: Methodsmentioning

confidence: 99%

Duration of Enterovirus D68 RNA Shedding in the Upper Respiratory Tract and Transmission among Household Contacts, Colorado, USA

Nguyen-Tran,

Thompson,

Butler

et al. 2023

Emerg. Infect. Dis.

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Enterovirus D68 (EV-D68) causes cyclical outbreaks of respiratory disease and acute flaccid myelitis. EV-D68 is primarily transmitted through the respiratory route, but the duration of shedding in the respiratory tract is unknown. We prospectively enrolled 9 hospitalized children with EV-D68 respiratory infection and 16 household contacts to determine EV-D68 RNA shedding dynamics in the upper respiratory tract through serial midturbinate specimen collections and daily symptom diaries. Five (31.3%) household contacts, including 3 adults, were EV-D68–positive. The median duration of EV-D68 RNA shedding in the upper respiratory tract was 12 (range 7–15) days from symptom onset. The most common symptoms were nasal congestion (100%), cough (92.9%), difficulty breathing (78.6%), and wheezing (57.1%). The median illness duration was 20 (range 11–24) days. Understanding the duration of RNA shedding can inform the expected rate and timing of EV-D68 detection in associated acute flaccid myelitis cases and help guide public health measures.

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Section: Methodsmentioning

confidence: 99%