Qualitative and Quantitative Studies of Eosinophils in Parasitic Infections

Abstract

Th2 responses such as peripheral and tissue eosinophilia are characteristic features in the host animals infected with Strongyloides venezuelensis and Trichinella spiralis. Th2 responses are characterized by a specific profile of cytokines and chemokines induced during the course of infection. In this chapter, we describe the methodology that is utilized in our laboratories to study the production of cytokine, chemokine, and antibodies related to the eosinophilia seen in mice infected with the parasites. Furthermore, protocols are described for the different methods used to study eosinophil functions in the blood and tissues of these experimental models of parasitic infections.