Qualitative and quantitative strategies of thermal adaptation of grass carp (Ctenopharyngodon idella) cytoplasmic malate dehydrogenases

Lin, Jen-Jen; MacLeod, Shirley; Kuo, Chun-Yi

doi:10.1007/bf01874840

Cited by 3 publications

(1 citation statement)

References 31 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to behavior, however, ectotherms may exhibit physiological mechanisms to address thermal constraints of their environment. Solutions for surviving inhospitable temperatures may include manipulating biochemical reaction rates by varying enzyme concentrations or receptor densities, production of chaperone proteins to increase the range of temperatures over which target enzymes remain functional [3], changing the composition of cell membranes to affect permeability and (rarely) producing temperature-specific isozymes [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Interspecific Variation in Temperature Effects on Embryonic Metabolism and Development in Turtles

Ligon

Lovern

2012

ISRN Zoology

View full text Add to dashboard Cite

We measured temperature-induced differences in metabolic rates and growth by embryos of three turtle species,Macrochelys temminckii, Trachemys scripta, andApalone spinifera, at different, constant, temperatures. Oxygen consumption rate (VO2) was measured during development and used to characterize changes in metabolism and calculate total O2consumption. Results from eggs incubated at different temperatures were used to calculate Q10s at different stages of development and to look for evidence of metabolic compensation. Total O2consumption over the course of incubation was lowest at high incubation temperatures, and late-term metabolic rate Q10s were <2 in all three species. Both results were consistent with positive metabolic compensation. However, incubation temperature effects on egg mass-corrected hatchling size varied among species.Apalone spiniferahatchling mass was unaffected by temperature, whereasT. scriptamass was greatest at high temperatures andM. temminckiimass was lowest at high temperatures. Hatchling mass : length relationships tended to correlate negatively with temperature in all three species. Although we cannot reject positive metabolic compensation as a contributor to the observed VO2patterns, there is precedence for drawing the more parsimonious conclusion that differences in yolk-free size alone produced the observed incubation temperature differences without energetic canalization by temperature acclimation during incubation.

show abstract

Section: Introductionmentioning

confidence: 99%

Interspecific Variation in Temperature Effects on Embryonic Metabolism and Development in Turtles

Ligon

Lovern

2012

ISRN Zoology

View full text Add to dashboard Cite

show abstract

Phylogenetic Relationships and Biochemical Properties of the Duplicated Cytosolic and Mitochondrial Isoforms of Malate Dehydrogenase from a Teleost Fish, Sphyraena idiastes

et al. 2002

View full text Add to dashboard Cite

Unlike birds and mammals, teleost fish express two paralogous isoforms (paralogues) of cytosolic malate dehydrogenase (cMDH; EC 1.1.1.37; NAD+: malate oxidoreductase) whose evolutionary relationships to the single cMDH of tetrapods are unknown. We sequenced complementary DNAs for both cMDHs and the mitochondrial isoform (mMDH) of the fish Sphyraena idiastes (south temperate barracuda) and compared the sequences, kinetic properties, and thermal stabilities of the three isoforms with those of mammalian orthologues. Both fish cMDHs comprise 333 residues and have subunit masses of approximately 36 kDa. One cytosolic isoform, cMDH-S, was significantly more heat-stable than either the other cMDH (cMDH-L) or mMDH. In contradiction to the generally accepted model of vertebrate cMDH evolution, our phylogenetic analysis indicates that the duplication of the fish cytosolic paralogues occurred after the divergence of the lineages leading to teleosts and tetrapods. cMDH-L and cMDH-S differed in optimal concentrations of substrates and cofactors and apparent Michaelis-Menten constants, suggesting that the two paralogues may play distinct physiological roles. Differences in intrinsic thermal stability among MDH paralogues may reflect different degrees of stabilization in vivo by extrinsic stabilizers, notably protein concentration in the case of mMDH. Thermal stabilities of porcine mMDH and cMDH-L, but not cMDH-S, were significantly increased when denaturation was measured at a high protein (bovine serum albumin; BSA) concentration, but the BSA-induced stabilization reduced the catalytic activity.

show abstract

Cloning and primary structure of putative cytosolic and mitochondrial malate dehydrogenase from the mollusc Nucella lapillus (L.)

Kirby

2000

Gene

View full text Add to dashboard Cite

Qualitative and quantitative strategies of thermal adaptation of grass carp (Ctenopharyngodon idella) cytoplasmic malate dehydrogenases

Cited by 3 publications

References 31 publications

Interspecific Variation in Temperature Effects on Embryonic Metabolism and Development in Turtles

Interspecific Variation in Temperature Effects on Embryonic Metabolism and Development in Turtles

Phylogenetic Relationships and Biochemical Properties of the Duplicated Cytosolic and Mitochondrial Isoforms of Malate Dehydrogenase from a Teleost Fish, Sphyraena idiastes

Cloning and primary structure of putative cytosolic and mitochondrial malate dehydrogenase from the mollusc Nucella lapillus (L.)

Contact Info

Product

Resources

About