Qualitative and quantitative peptidomic and proteomic approaches to phenotyping chicken semen

Labas, Valérie; Grasseau, Isabelle; Cahier, Karine; Gargaros, Audrey; Harichaux, Grégoire; Teixeira-Gomes, Ana-Paula; Alves, Sabine; Bourin, M.; Gérard, Nadine; Blesbois, Élisabeth

doi:10.1016/j.jprot.2014.07.024

Cited by 85 publications

(120 citation statements)

References 80 publications

Supporting

Mentioning

111

Contrasting

Order By: Relevance

“…The major proteins identified in the SF of all breeds were serum albumin and ovotransferrin, thus confirming previous studies (Marzoni et al, 2013;Labas et al, 2015). Expectedly too, spermadhesins were not present owing to the lack of sexual accessory producing glands in chicken, contrasting with the boars (and other mammals) where these proteins of seminal vesicle main origin dominate (Rodriguez-Martinez et al, 2011).…”

Section: Discussionsupporting

confidence: 84%

“…Comparative examinations of the proteome of chicken are scarce and mostly identified major proteins in common commercial breeds (Marzoni et al, 2013;Labas et al, 2015). Moreover, eventual relationships of the SF proteome with fertility (egg-laying capacity) are yet to be studied in detail.…”

Section: Discussionmentioning

confidence: 99%

“…The SF/SP signaling proteins and peptides -i.e. avian β-defensins (Marzoni et al, 2013;Labas et al, 2015); or porcine spermadhesins (Rodríguez-Martínez et al, 2010;Perez-Patiño et al, 2016); cytokines/chemokines (pig: O´Leary et al, 2011;Jiwakanon and Dalin, 2012), and or sex hormones (progesterone, testosterone, dihydrotestosterone and estrogen) (chicken: Anderson and Navara, 2011;Oliveira et al, 2011;pig: Claus et al, 1983;Zdunczyk et al, 2011), are all considered to contribute to the orchestration of such local immune defence responses in the female genitalia.…”

Section: Semen Is Immunologically Foreign To the Femalementioning

confidence: 99%

“…Avian β-defensins (AvβD-9 and AvβD-10) have been identified in the SF as well as on the sperm-surface (AvβD-3) and are classified as immunity/defence proteins (Marzoni et al, 2013;Labas et al, 2015). AvβDs 1-5 and 7-12 are important factors in the innate and adaptive immunity (Sugiarto and Yu, 2004;Das et al, 2008;, expressed by the oviduct of hens (Abdel-Mageed et al, 2008;Shimizu et al, 2008).…”

Section: Semen Is Immunologically Foreign To the Femalementioning

confidence: 99%

“…Among these, the transforming growth factor-β (TGF-β1-2), interferon-γ (IFN-γ) and interleukins (IL)-6 and IL-10, show quantitative variation between ejaculate fractions (O' Leary et al, 2011;Jiwakanon and Dalin, 2012). In chicken, proteomic/peptidomic studies of seminal fluid are scarce (Marzoni et al, 2013;Labas et al, 2015). Comparative cytokine/chemokine studies are also restricted to cytokine expression in the testis (Ocón-Grove et al, 2010;Michailidis et al, 2014) and the female genitalia.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Atikuzzaman¹

View full text Add to dashboard Cite

Internal fertilization occurs in birds and eutherian mammals. Foetal development, however, is either extra-respectively intra-corpore (egg vs uterus). In these animal classes, the female genital tract stores ejaculated spermatozoa into a restricted oviductal segment; the functional pre-ovulatory sperm reservoir, where they survive until ovulation/s occur. Paradoxically, this immunologically foreign sperm suspension in seminal fluid/plasma, often microbiologically contaminated, ought to be promptly eliminated by the female local immune defence which, instead, tolerates its presence. The female immune tolerance is presumably signalled via a biochemical interplay of spermatozoa, as well as the peptides and proteins of the extracellular seminal fluid, with female epithelial and immune cells. Such interplay can result in gene expression shifts in the sperm reservoir in relation to variations in fertility. To further aid our understanding of the underlying mechanisms, this thesis studied the proteome of the seminal fluid (using 2D SDS-PAGE and mass spectrometry) including cytokine content (using Luminex and/or ELISA) of healthy, sexually mature and fertile boars and cocks. As well, gene expression changes (using cDNA microarray) in the oviductal sperm reservoirs of sexuallymature females, mated or artificially infused with homologous sperm-free seminal fluid/plasma were studied. Pigs were of commercial, fertility-selected modern breeds (Landrace), while chicken belonged to the ancestor Red Junglefowl (RJF, low egg laying-capacity), a selected egg-layer White Leghorn (WL) and of their Advanced Intercross Line (AIL). Ejaculates were manually collected as single sample in cocks or as the sperm-rich fraction [SRF] and the post-SRF fraction in boars to harvest seminal fluid/plasma for proteome/cytokine and infusionstudies. Oviducts were retrieved for gene-expression analyses via microarray immediately post-mortem (chicken) or at surgery (pig), 24 h after mating or genital infusion. In pigs, the protein-rich seminal plasma showed the highest amounts of cytokines [interferon-γ, interferon gamma-induced protein 10 (IP-10/CXCL10), macrophage derived chemokine (MDC/CCL22), growth-regulated oncogene (GRO/CXCL1), granulocyte-macrophage colony-stimulating factor (GM-CSF), monocyte chemo-attractant protein-1 (MCP-1/ CCL2), interleukin (IL)-6, IL-8/CXCL8, IL-10, IL-15, IL-17 and transforming growth factor (TGF)-β1-3) in the larger, protein-rich and sperm-poor post-SRF, indicating its main immune signalling influence. Chicken showed also a plethora of seminal fluid proteins with serum albumin and ovotransferrin being conserved through selection/evolution. However, they showed fewer cytokines than pigs, as the anti-inflammatory/immune-modulatory TGF-β2 or the proinflammatory CXCL10. The RJF contained fewer immune system process proteins and lacked TGF-β2 compared to WL and AIL, suggesting selection for increased fertility could be associated with higher expression of immune-regulating peptides/proteins. The oviductal sperm re...

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Semen Is Immunologically Foreign To the Femalementioning

confidence: 99%

Section: Semen Is Immunologically Foreign To the Femalementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Atikuzzaman¹

View full text Add to dashboard Cite

show abstract

Three‐dimensional structures of avian beta‐microseminoproteins: insight from the chicken egg‐specific beta‐microseminoprotein 3 paralog

et al. 2021

Self Cite

View full text Add to dashboard Cite

Beta-microseminoproteins (MSMBs) are small disulfide-rich proteins that are conserved among vertebrates. These proteins exhibit diverse biological activities and were mainly reported to play a role in male fertility, immunity, and embryogenesis. In this work, we focused on the chicken MSMB3 protein that was previously depicted as an egg antibacterial protein. We report that MSMB3 protein is exclusively expressed in the reproductive tissues of laying hens (in contrast to chicken MSMB1 and MSMB2 paralogs), to be incorporated in the egg white during the process of egg formation. We also showed that chicken MSMB3 possesses highly conserved orthologs in bird species, including Neognathae and Palaeognathae. Chicken MSMB3 was purified from egg white using heparin affinity chromatography and was analyzed by top-down and bottom-up proteomics. Several proteoforms could be characterized, and a homodimer was further evidenced by NMR spectroscopy. The X-ray structure of chicken MSMB3 was solved for the first time, revealing that this protein adopts a novel dimeric arrangement. The highly cationic MSMB3 protein exhibits a distinct electrostatic distribution compared with chicken MSMB1 and MSMB2 structural models, and with published mammalian MSMB structures. The specific incorporation of MSMB3 paralog in the egg, and its phylogenetic conservation in birds together with its peculiar homodimer arrangement and physicochemical properties, suggests that the MSMB3 protein has evolved to play a critical role during the embryonic development of avian species. These new data are likely to stimulate research to elucidate the structure/function relationships of MSMB paralogs and orthologs in the animal kingdom.Beta-microseminoproteins (MSMBs) are nonglycosylated disulfide-rich miniproteins (molecular mass about 10 kDa) that have been identified in many animal species [1,2]. MSMB genes have been reported to exhibit strong conservation among vertebrates, although they share a relatively low level of sequence identity Abbreviations MALDI-TOF, matrix-assisted laser desorption ionization-time of flight; MSMB, microseminoprotein; nanoLC-MS/MS, nanoflow liquid chromatography-tandem mass spectrometry; PDB, protein data bank; PSP94, prostate secretory protein 94; SSP-2, small serum protein 2; TOCSY, total correlation spectroscopy.

show abstract

The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken

Thélie

Réhault-Godbert

Poirier

et al. 2019

Molecular Reproduction Devel

Self Cite

View full text Add to dashboard Cite

The seminal plasma is a very complex fluid, which surrounds sperm in semen. It contains numerous proteins including proteases and protease inhibitors that regulate proteolytic processes associated with protein activation and degradation. We previously identified a seminal protein, chicken liver trypsin inhibitor 1 (ClTI‐1) over expressed in semen of roosters with high fertility, suggesting a role in male fertility. In the present study, we showed that ClTI‐1 gene is actually SPINK2. Using normal healthy adult roosters, we showed that SPINK2 amount in seminal plasma was positively correlated with male fertility in chicken lines with highly contrasted genetic backgrounds (broiler and layer lines). Using affinity chromatography combined to mass spectrometry analysis and kinetic assays, we demonstrated for the first time that two chicken acrosin isoforms (acrosin and acrosin‐like proteins) are the physiological serine protease targets of SPINK2 inhibitor. SPINK2 transcript was overexpressed all along the male tract, and the protein was present in the lumen as expected for secreted proteins. Altogether, these data emphasize the role of seminal SPINK2 Kazal‐type inhibitor as an important actor of fertility in birds through its inhibitory action on acrosin isoforms proteins.

show abstract

Qualitative and quantitative peptidomic and proteomic approaches to phenotyping chicken semen

Cited by 85 publications

References 80 publications

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Three‐dimensional structures of avian beta‐microseminoproteins: insight from the chicken egg‐specific beta‐microseminoprotein 3 paralog

The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken

Contact Info

Product

Resources

About