Qualitative and Quantitative Histoenzymology of Long Surviving Human Renal Homotransplants

Abstract

The enzymology of four human kidneys grafted for 7, 8, 15 and 21 months and removed because of progressive, irreversible decrease in glomerular filtration has been studied by microscopic histoenzymology and by quantitative histoenzymology, enzymatic determinations being made on glomeruli and proximal convoluted tubules separately. The main feature of histoenzymology is the disappearance of succinic dehydrogenase in all the proximal convoluted tubules of sharply delineated zones, which seem to correspond to the severest endarterial obstructive lesions. Quantitative estimations were made for four enzymes: LDH, ICDH, G-6-PDH and acid P’ ase. In glomeruli, there is a decrease of ICDH and acid P’ ase, LDH is practically unchanged and G-6-PDH is moderately increased. In the tubules, there is a moderate decrease in LDH, a striking decrease in ICDH, an increase of G-6-PDH more important than in the glomeruli and practically no change in acid P’ ase. The significance of these findings are discussed as being related to anoxia and, possibly, to antigen-antibody reaction as well.

Catalytic Activities of Alkaline Phosphatase and N-Acetyl-ß-D-Glucosaminidase in Human Cortical Nephron Segments: Heterogeneous Changes in Acute Renal Failure and Acute Rejection Following Kidney Allotransplantation

“…The changes were found to be very heterogeneous according to the histological appearance of the tissue. Figure l demonstrates this heterogeneity with respect to round-cell Infiltration and succinate dehydrogenase activity, äs also described by Gregoire & Gepts (13). Since microdissection was done in sections adjacent to the stained sections, isolated specimens could be correlated to normal or reduced succinate dehydrogenase activity (19).…”

“…Enzymuria in-structures in proximal and distal tubules (11). In creases, when renal function is impaired (l, 2, 3), addition, Gregoire & Gepts (13) observed reduced indicating renal damage. However, the renal pro-activity of microvillar alkaline phosphatase and uncesses involved in enzymuria are not yet understood.…”

Studies of Human Kidney and Urine Acid Phosphatase

“…Pathological changes in the glomerulus have frequently been described as a manifestation of chronic rejection [13]. In a recent study of histoenzymologic changes in long-surviving human renal homografts a decrease in glomerular acid phosphatase activity was noted [12]. It was proposed that a sustained antigen-antibody reaction on the surface of glomerular endothelial cells might solubilize acid phosphatase and favor its leakage out of these cells.…”