Qualitative and quantitative evaluation of ginkgo terpene lactone raw material by HPLC/Q-TOF MS combined with HPLC-DAD-ELSD

Abstract

Characterization and identification of chemical components in GTL by HPLC/Q-TOF MS/MS combined with online valve switching.

Simultaneous determination of ginkgolides A, B and K in human plasma by UPLC-MS/MS and its application to the pharmacokinetic study of Ginkgo Diterpene Lactone Meglumine Injection in humans

“…The developed method was applied to a pharmacokinetic study in health subjects following a single intravenous infusion of Ginkgo Diterpene Lactone Meglumine Injection (containing 25 mg ginkgolides of 8.5 mg ginkgolide A, 14.5 mg ginkgolide B, 1.0 mg ginkgolide K and 1.0 mg other ginkgolides 11 ). The study was conducted at a single study center (First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China).…”

“…In this injection, a ginkgo terpene lactone raw material (mainly containing ginkgolides A, B and K) is dissolved in 0.5% meglumine in saline (m/v), and the ginkgolides are present as hydrolyzed forms with one or two lactone groups hydrolyzed. 11,12 A previous study evaluated in vitro inhibition and induction of cytochrome P450 activities by hydrolyzed ginkgolides, while, in this study the drug concentrations in the in vitro test were expressed as the total concentrations of the hydrolyzed ginkgolides (the sum of equivalent concentrations of ginkgolides A, B and K). Since both hydrolyzed ginkgolides and lactones existed in human plasma upon the administration of hydrolyzed ginkgolides or lactones, it is necessary to quantify the hydrolyzed ginkgolides and lactones separately in plasma in the pharmacokinetic studies.…”

Qualitative and quantitative analysis of major constituents from Dazhu Hongjingtian capsule by UPLC/Q‐TOF‐MS/MS combined with UPLC/QQQ‐MS/MS

“…Because of the complexity, similarity and diversity of the chemical structures, the analysis of DHC is a great challenge. Recently, ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UPLC/Q‐TOF MS/MS) has become a powerful tool for identifying chemical components in TCMs (Wu et al, ; Zhao et al, ). With the application of Q‐TOF, accurate mass measurements could be performed, the molecular formula could be calculated and molecule fragments could be differentiated (Gampe, Darcsi, Lohner, Béni, & Kursinszki, ).…”