Qualitative and quantitative enzymatic peptide synthesis, using Pseudomonas aeruginosa elastase

Kinetic study of the pseudolysin-catalyzed synthesis of Z-Ala-Phe-NH2

“…Pseudomonas aeruginosa elastase (pseudolysin), a thermolysin-like metalloproteinase has been shown to synthesize N-protected dipeptide amides with good yields and purities [3,4]. While both enzymes show some structural similarities [5] elastase but not thermolysin can incorporate tyrosine and tryptophane in P' 1 position with high synthesis rates (S. Rival unpublished).…”