Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI–MS/MS)

Fan, Haidong; Li, Shao‐Ping; Jin, Xiang; Lai, Chang-Jiang-Sheng; Yang, Feng‐Qing; Gao, Jian‐Li; Wang, Y.T.

doi:10.1016/j.aca.2006.03.032

Cited by 95 publications

(87 citation statements)

References 24 publications

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“…Actually, many works for the qualitative and quantitative determination of nucleosides and their bases in Cordyceps have been reported. Several methods, including HPLC-DAD (diode array detector) [4,5], CE [6], CEC [7], LC-MS [8], ultra performance liquid chromatography [9], have been used for nucleosides analysis in Cordyceps. Sterols, especially ergosterol, have also been determined using HPLC-DAD [5], LC-MS [10] and GC-MS [11].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Wang

Yang

Feng

et al. 2009

J of Separation Science

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A HPLC coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) method for qualitative and quantitative analysis of eight nucleosides and nucleobases, three carbohydrates and myriocin in Cordyceps was developed. A Prevail Carbohydrate ES column was employed for the separation within 50 min. Nucleosides and their bases were tested at UV 254 nm. ELSD was connected with DAD to determine myriocin and carbohydrates. The optimum drift tube temperature of ELSD was at 941C with the nitrogen flow rate of 2.0 L/min. All calibration curves showed good linearity (R 2 40.9933) during the test ranges. The precision, repeatability, accuracy, LOD and LOQ were also fully investigated. This developed method was successfully applied to quantify 12 components, eight nucleosides and nucleobases, three carbohydrates and myriocin, in natural and cultured Cordyceps, which provides another view for quality control of Cordyceps sinensis.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Wang

Yang

Feng

et al. 2009

J of Separation Science

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“…Guanine (0.2 mg/mL) was prepared in 0.1 mol/L HCl (Fan et al 2006). The mixed standard stock solution was prepared by adding the standard stock solutions of the analytes together.…”

Section: Methodsmentioning

confidence: 99%

“…The profile of nucleosides, nucleobases and uniquely modified nucleosides, instead of any single metabolite, can be feasible for the quality control of Cordyceps sensu lato products (Shiao et al 1994; Xiao and Xiong 2013). The nucleoside profile of Ophiocordyceps species and its allies has been explored, and the analytical methods of determining nucleosides and nucleobases in Cordyceps have been reported previously (Li et al 2004; Fan et al 2006; Yang et al 2007, 2009; Xiao and Xiong 2013; Zhang et al 2014; Qian and Li 2017). High performance liquid chromatography (HPLC), combined with ultraviolet (UV), evaporative light scattering detector or mass spectra, has been applied to analyse the profile of nucleosides, nucleobases in Cordyceps -related species, and proved to be a simple, convenient and reliable method.…”

Section: Introductionmentioning

confidence: 99%

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

et al. 2017

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Nucleosides and nucleobases are one of the most important indicators of quality control. A sensitive and reliable high performance liquid chromatography-ultraviolet method was applied to analyse 13 nucleosides and nucleobases simultaneously in 15 batches of nine Ophiocordyceps species and its allies in China. Principal component analysis (PCA) and cluster analysis were conducted by SPSS 22.0 software (IBM Corp., Armonk, NY, USA). The 15 samples of Cordyceps were differentiated successfully based on their nucleoside and nucleobase content. Total nucleosides content in mycelium was significantly higher than that in the natural fruiting bodies of Ophiocordyceps sinensis (NFOS). Five nucleosides or nucleobases – adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) – were the major components contributed to the total variance according to PCA. The profiles of the 13 tested nucleosides and nucleobases (including adenosine, cytidine, guanosine, inosine, thymidine, uridine, cordycepin, adenine, cytosine, guanine, thymine, uracil and hypoxanthine) can discriminate different samples and can be candidate indicators applied for the quality control of Ophiocordyceps and its allies.

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“…Descriptions of selected reaction monitoring (SRM) transitions can be found in previous studies and are summarized in Table 3. (Lee et al 2004;Su et al 2014;Fan et al 2006) The LC-MS/MS system was controlled by Xcalibur software (Thermo Fisher Scientific), which was also used to collect data.…”

Section: Lc-ms/ms Conditionsmentioning

confidence: 99%

Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

et al. 2017

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Background: The profiles of modified nucleosides could act as useful biomarkers for cancer and cellular stressinduced diseases. However, there are no reports of high throughput and simultaneous quantitative methods for using biomarker evaluation and discovery at the bedside. Methods: Modified nucleosides were separated on two CAPCELL PAK ADME S3 (100 mm × 2.1 mm i.d.; 3-μm particle size) analytical columns coupled with a CAPCELL PAK ADME cartridge (10 mm × 2 mm i.d.; 3-μm particle size) guard column. Both columns were used in tandem during multi-column LC analysis to reduce analysis time. Two mobile phases were used, including 20 mM ammonium acetate adjusted to pH 5.3 using acetic acid and 1. 0 M ammonium acetate/acetonitrile/water/acetic acid (1/95/5/0.03, v/v/v/v), with the post-column addition of methanol to enhance ionization efficiency. Tandem mass spectrometry detection was performed using a triple quadrupole mass spectrometer equipped with a heated electrospray ionization source in selected reaction monitoring mode. Results: Four major nucleosides and 11 modified nucleosides, including guanosine, adenosine, uridine (U), cytidine, inosine, 1-methyladenosine, 5-methylcytidine, 2′-O-methylcytidine, 3-methylcytidine, 7-methylguanosine (m 7 G), 5-methyluridine (m 5 U), pseudouridine, 2-thiocytidine, N 2 -methylguanosine (m

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Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI–MS/MS)

Cited by 95 publications

References 24 publications

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

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