Qualitative and Quantitative Determination of Secondary metabolites and Antioxidant Potential of Eruca sativa

Abstract

Objective: To Determine the phytochemical composition of E. sativa (stem, leaves, flowers and seeds), and evaluate their antioxidant activity. Method: Preliminary phytochemical screening for all parts of E. sativa (stem, leaves, flowers and seeds) was carried out according to standard methods. Total phenolic contents of all methanolic extracts of E. sativa, have been quantified spectrophotometrically. Hydrogen Peroxidase and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical assays have been used to analyze antioxidant characteristics of all extracts of E. sativa (leaves, stem, seed, flowers and seeds). Further separation and identification of number of phenolic compounds has been carried out by Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). Results: Experimental evaluation indicated that E. sativa is a rich source of secondary phytoconstituents (Alkaloids, flavonoids, Diterpenes, Coumarins, polyphenols, tannins, cardiac glycosides etc). Quantification of total phenolic contents from all aerial parts revealed that they contain significant amount of phenolics particularly seeds and leaves (27.1 ± 0.2 mg, 23.07 ± 0.11GAE/g) respectively. Searation and identification of phenolics from E. sativa stem, leaves, flowers and seeds extracts through RP-HPLC showed presence of variety of important phenolics namely; Vanillin (RT=3.853), Ellagic acid (RT=4.04), Salicylic acid (RT=19.09), Resorcinol (RT=3.30), Catechol (RT=3.53), Quercetin (RT=18.91), Benzoic acid (RT=10.4), Tannic acid (RT=5.06), Kaempferol (RT=8.70) and Rutin (RT=9.2). Conclusion: Results revealed that E. sativa is a rich source of secondary phytoconstituents which impart significant antioxidant potential. This work also contributes significantly to support the claim about the use of this herb in folk medicines. Further investigation regarding isolation and purification of a number of phytoconstituents from leaves, stem, flowers and seeds of E. sativa may yield optimal combinations of therapeutic alternates.

Eruca sativa Meal against Diabetic Neuropathic Pain: An H2S-Mediated Effect of Glucoerucin

“…oleifera Mill seeds [24,25,26]. Despite the limited studies on erucin, beneficial properties similar to SFN have recently been reported [27,28,29], as its ability to release H 2 S in vitro and to mediate vasodilatation [30,31]. This also led to assume that the effects showed by SFN in vivo could actually be due to its rapid interconversion to erucin [32].…”

Phytochemical analysis and assessment of antioxidant, antimicrobial, anti-inflammatory and cytotoxic properties of Tetraclinis articulata (Vahl) Masters leaves

“…The diseases related with the oxidative stress, such as cancer and cardiovascular problems (Andrade et al, 2005;Azmi et al, 2006) has become a serious problem in recent years. Accordingly, natural antioxidant products obtained from medicinal herbs, such as phenolic compounds, vitamins and terpenoids have been widely applied in food and medicinal fields (Cai et al, 2004;Leandro et al, 2012;Sadiq, 2014). In particular, the antioxidant activity of polyphenols, mainly flavonoids, is related with free radical-scavenging capacity, strong linkage to free ferrous ions and antimutagenic effect through high inhibition of the oxidative DNA damage (Lodovici et al, 2001;Wu et al, 2007).…”

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“…Several biochemical constituents were evaluated qualitatively and quantitatively using standard protocols (Geetha and Geetha, 2014;Sadiq, 2014). …”