Qualitative and quantitative determination of biologically active low-molecular-mass thiols in human blood by reversed-phase high-performance liquid chromatography with photometry and fluorescence detection

Ivanov, A.R; Nazimov, Igor V.; Baratova, L. A.

doi:10.1016/s0021-9673(99)00947-4

Cited by 136 publications

(60 citation statements)

References 23 publications

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“…中同型半胱氨酸的增加将导致维生素 B12 的缺乏，甚至引起阿尔茨海默症 [6]。因此，检测硫醇具有重要意义。在过去的几十年中，检测硫醇的方法有很多，包括高效液相色谱法(HPLC) [7]、电化学方法 [8] [9]、毛细管电泳法(CE) [10]、质谱法(MS) [11]和荧光法 [12] …”

Section: 氧化应激和代谢功能起着至关重要的作用。谷胱甘肽水平也与许多其他的疾病直接相关[5]。此外，血液unclassified

A Highly Sensitive Squaraine-Based NIR Probe for Biothiols with Colorimetric and Ratiometric Changes

李¹

2018

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In order to detect high sensitivity and selective biothiol, we designed and synthesized a NIR probe based on a symmetrical squaraine derivative (Sq-TPE), with absorption and emission bands beyond 700 nm. Sq-TPE exhibits high sensitivity and selectivity towards biothiols over the other amino acids and bioanalytes. The addition of three biothiols results in significant color changes of Sq-TPE from cyan to yellow, and meanwhile ratiometric emission changes from red to orange. More excitingly, it shows detection limits as low as 9.8 nM for Cys, 12.4 nM for GSH and 9.7 nM for HCy, respectively.

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Section: 氧化应激和代谢功能起着至关重要的作用。谷胱甘肽水平也与许多其他的疾病直接相关[5]。此外，血液unclassified

A Highly Sensitive Squaraine-Based NIR Probe for Biothiols with Colorimetric and Ratiometric Changes

李¹

2018

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“…In recent years, a great amount of interesting studies have focused on the determination and sensing of biothiols. Various analytical methods including high-performance liquid chromatography [10,11], capillary electrophoresis [12,13], mass spectrometry [14], chemiluminescence [15,16], electrochemistry assay [17,18], fluorimetry [19][20][21], and nanoparticles-based colorimetric sensing strategy [22][23][24][25][26][27] for biothiols have been developed. Among these methods, fluorescent probes and gold-nanoparticle-based colorimetric sensing gradually stand out in the rapid, sensitive, and selective determination of biothiols.…”

Section: Introductionmentioning

confidence: 99%

FITC functionalized magnetic core–shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols

Chen

Wang

et al. 2014

Sensors and Actuators B: Chemical

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a b s t r a c tA sensing strategy for chromogenic detection of molecular biothiols has been proposed based on fluorescein isothiocyanate (FITC) functionalized magnetic core-shell Fe 3 O 4 /Ag hybrid nanoparticles. Ag coated magnetic Fe 3 O 4 nanoparticles were initially synthetized. FITC was subsequently conjugated on the surface of core-shell nanoparticles by Ag-SCN linkage and then the fluorescence of FITC was quenched. Upon addition of molecular biothiols, since the Ag S bond is stronger than Ag-SCN, a place-displacement between thiols and FITC would occur and thereby the fluorescence of FITC would recover. Thus, a fluorescence "off-on" probe was attained by virtue of biothiols, so after magnetic separation, the fluorescence signal change of FITC in clear solution could be employed for quantitative determination of typical molecular biothiols such as glutathione (GSH) and cysteine (Cys). High sensitivity was obtained with the detection limits of 10 nM and 20 nM for GSH and Cys, respectively. As well as, the assay strategy presented excellent selectivity toward molecular biothiols against other amino acids. Furthermore, confocal imaging was achieved in living cells, indicating that this fluorescent probe is potentially applicable for imaging molecular thiols in biological systems.

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“…mass spectrometry, 11 nuclear magnetic resonance, 12 gas chromatography, 13 liquid chromatography, [14][15][16][17][18][19][20] and radio-immuno-assay procedures. 21 Metal ions, especially soft metals, such as copper, silver, mercury and palladium, have a high affinity for -SH groups in organic ligands.…”

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confidence: 99%

Spectrophotometric Determination of Biologically Active Thiols with Eosin, Silver(I) and Adenine

2002

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Compounds having a mercapto group (thiol) widely exist in animals, plants and microorganisms, and are well known to play important roles in biological and pharmaceutical process, e.g., acting as a detoxication agent, a protection agent and so on. Hence, various drugs contain naturally occurring thiols for the above-described purpose. Methods for analyzing these thiols include titrimetry, 1 spectrophotometry, 2-8 spectrofluorometry, 9 electroanalysis, 10 mass spectrometry, 11 nuclear magnetic resonance, 12 gas chromatography, 13 liquid chromatography, [14][15][16][17][18][19][20] and radio-immuno-assay procedures. 21 Metal ions, especially soft metals, such as copper, silver, mercury and palladium, have a high affinity for -SH groups in organic ligands. We have already reported on fluorophotometric determinations of sulfide ion 22 and penicillins 23 with fluorescein-mercury compounds, and on spectrophotometric determinations of thiourea 24 and β-lactam compounds 25 with palladium(II) and a dye. However, the former method, based on mercury, has a limit for its possible toxicity, and the latter method, based on palladium, has a problem concerning the influences of foreign substances. On the other hand, we have reported on simple and highly spectrophotometric determinations of adenine 26 and silver(I) 27 based on the ternary complex formation of eosin-silver(I)-adenine. In this reaction system, we noticed that the analytical values of adenine or silver(I) were severely interfered with by small amounts of thiols, and were only little interfered with by almost other coexisting substances. We speculated that a method which would utilize the fading of a eosin-silver(I)-adenine ternary complex in the presence of a thiol would serve as a simple and highly sensitive determination of thiols. Here, we report on a spectrophotometric method for the determination of biologically active thiols based on the fading of a eosinsilver(I)-adenine complex in the presence of thiols. Experimental Reagents and solutions6-Mercaptopurine (MP) and other thiols, analytical-grade reagents, were purchased from Wako Pure Chemical Co., Ltd., Sigma Chemical Co., Nacalai Tesque Inc., and Kishida Chemical Co., Ltd., and used without further purification. The stock and working solutions of other thiols were freshly prepared every week. A stock solution of silver(I) (1.0 × 10 -2 M, 1 M = 1 mol dm -3 ) was prepared by dissolving 108 mg of metallic silver (99.99%, Mitsuwa Chemicals) in 2 ml of 60% nitric acid by heating; this solution was diluted to 100 ml with water. The working solution (2.0 × 10 -4 M) was made by suitable dilution of this stock solution, as required. An adenine solution (2.0 × 10 -3 M) was prepared by dissolving a proper quantity of adenine (Sigma Chemical Co.) in 2 ml of 10% sulfuric acid and diluting the mixture to 100 ml with water. A solution (1.0 × 10 -3 M) of eosin was prepared by dissolving eosin (Wako Pure Chemical Co., Ltd.) in water. A solution of poly(N-vinylpyrrolidone) (PVP, K-90, Kishida Chemical Co., Ltd.) was prepared...

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Qualitative and quantitative determination of biologically active low-molecular-mass thiols in human blood by reversed-phase high-performance liquid chromatography with photometry and fluorescence detection

Cited by 136 publications

References 23 publications

A Highly Sensitive Squaraine-Based NIR Probe for Biothiols with Colorimetric and Ratiometric Changes

A Highly Sensitive Squaraine-Based NIR Probe for Biothiols with Colorimetric and Ratiometric Changes

FITC functionalized magnetic core–shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols

Spectrophotometric Determination of Biologically Active Thiols with Eosin, Silver(I) and Adenine

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