Qualitative and quantitative determination of ten alkaloids in traditional Chinese medicine Corydalis yanhusuo W.T. Wang by LC–MS/MS and LC–DAD

Ding, Bo; Zhou, Tingting; Fan, Guorong; Hong, Zhanying; Wu, Yutian

doi:10.1016/j.jpba.2007.06.009

Cited by 101 publications

(71 citation statements)

References 9 publications

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“…The HPLC detection for yanhusuo was achieved by an Agilent SB C18 column (250 mm x 4.6 mm, id=5 μm) along with a pre-column filled with the same stationary phase. A linear gradient elution of A (0.2% acetic acid solution, adjusted with triethylamine to pH 5.0) and B (acetonitrile) was used according to the following profile: 0-8 min, 20% B; 8-25 min linear increase to 80% B; 25-30 min linear decrease to 20% B; 30-37 min 20% B (15). The extracts were solved with 50% acetonitrile in 10 mg/ml and filtered through a 0.45 μm membrane filter before delivered into the system.…”

Section: Methodsmentioning

confidence: 99%

A traditional Chinese medicine formulation consisting of Rhizoma Corydalis and Rhizoma Curcumae exerts synergistic anti-tumor activity

Wang¹

2009

Oncol Rep

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Abstract. Synergy analysis of anticancer agents is an important approach to determining the ratio and/or dose of drugs for clinical combination therapy. However, this method is rarely used to evaluate the composition of traditional Chinese medicine formulation. 'Yanhusuo San' (YHSS), which consists of yanhusuo (Rhizoma Corydalis) and Ezhu (Rhizoma Curcumae), has been an archaic Chinese medicine prescription since the Song dynasty (960-1279 AD). We previously demonstrated that either yanhusuo or ezhu has strong anticancer effect. Herein, we sought to determine the possible synergic effect between these two Chinese herbs. We measured the IC 50 of each herb extract and both extracts at different ratios of doses by MTT assay. Isobologram and combination index (CI) analyses were used to evaluate the synergistic effect of yanhusuo and ezhu in different fixed ratios. Our results indicated that a combination of two herbal extracts exhibits the strongest anticancer cell proliferation effect at the ratio of 3:2 (ezhu to yanhusuo; referred to as E3Y2). Using Boyden Chamber assay, flow cytometry, and fluorescence microscopy analysis, we found that E3Y2 could markedly reduce the cell invasion ability and induce cytochrome c release rather than single use, but E3Y2 could not influence the cell cycle distribution. When the levels of ERK1/2, p-ERK1/2 and p-Rb were determined by Western blot analysis, we found that the E3Y2 significantly suppresses the level of p-ERK. Thus, our studies provide a plausible molecular basis of the synergistic anti-tumor effect of ezhu and yanhusuo.

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Section: Methodsmentioning

confidence: 99%

A traditional Chinese medicine formulation consisting of Rhizoma Corydalis and Rhizoma Curcumae exerts synergistic anti-tumor activity

Wang¹

2009

Oncol Rep

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“…Several solvent systems, based on the mixtures of acetonitrile or methanol with water at different ratios, were tested to achieve optimal separation in a relatively short time. Better separation efficiency and sensitivity were achieved with acetonitrile than methanol [21]. Using 0.2% acetic acid and triethylamine in the solvent system can reduce the ionization of alkaloids and give more symmetrical peaks and a better separation [31,32].…”

Section: Analysis Of the Samplesmentioning

confidence: 99%

“…Several analytical methods have been developed and used for the detection of analogous alkaloids in Corydalis yanhusuo, a plant from the same genus as that of C. decumbens, such as high-performance liquid chromatography (HPLC) [17][18][19][20], liquid chromatography-mass spectrometry (LC-MS) [21][22][23], capillary electrophoresis (CE) [24,25], and high-speed countercurrent chromatography (HSCCC) [26]. However, only a few quantificational studies of C. decumbens alkaloids have been published.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of eleven alkaloids in Corydalis decumbens by HPLC with diode-array detection

Gu¹,

Yang²,

Wang³

et al. 2017

Acta Chromatographica

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Summary.A high-performance liquid chromatography-diode-array detection method was developed and validated to determine simultaneously eleven major alkaloids in Corydalis decumbens (Thunb.) Pers. The alkaloids detected were corlumidine, protopine, coptisine, tetrahydrojatrorrhizine, palmatine, berberine, sanguinarine, papaverine hydrochloride, tetrahydropalmatine, bicuculline, and corydaline. Chromatographic separation was achieved using a C-18 column with a mobile phase composed of A (0.2% acetic acid solution, adjusted with triethylamine to pH 5.0) and B (acetonitrile), with stepwise gradient elution. Ultraviolet diode-array detection was used; chromatograms were examined at the wavelength of 280 nm. The regression equations showed a good linear relationship between the peak area of each marker and concentration (r = 0.9994-0.9999). The recovery values ranged between 93.66% and 100.54%. The method was fully validated with respect to detection and quantification limits, precision, reproducibility, and accuracy. The described high-performance liquid chromatography (HPLC) method was successfully used for the differentiation and quantification of the eleven major alkaloids in C. decumbens (Thunb.) Pers. and can be considered an effective procedure for the analyses of this important class of natural compounds.

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“…Chromatographic separation, modified from Ding et al [16], was carried out on a Phenomenex (250 × 4.6 mm, Gemini 5 μm, C18, 110A, SN: 289246-5). The mobile phase consisted of A (0.2% aqueous acetic acid, adjusted with triethylamine to pH 5.0) and B (acetonitrile), using a gradient elution of 20% B holding for 8 min, 20%-36% B within 8-23 min, 36%-80% B within 23-40 min, 80%-95% B within 40-41 min, and 95%-95% B within 41-45 min.…”

Section: Quantitative Analysis Of the Alkaloid Content Of Corydalis Rmentioning

confidence: 99%

“…Several high performance liquid chromatography (HPLC) methods have been reported for the analysis of alkaloids in C. yanhusuo. Quantification is typically performed using ultraviolet (UV) detection while diode array detector (DAD) and mass spectrometry are often employed for the identification [15,16].…”

Section: Introductionmentioning

confidence: 99%

Influence of Vinegar and Wine Processing on the Alkaloid Content and Composition of the Traditional Chinese Medicine Corydalis Rhizoma (Yanhusuo)

Waldbauer

Tang

et al. 2014

Molecules

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Corydalis Rhizoma is the dried tuber of Corydalis yanhusuo W.T. Wang which is used in traditional Chinese medicine for pain relief and blood activation. Before being used in the clinics, C. yanhusuo is traditionally processed through dry-frying or frying with vinegar, wine or salt. In this study, eleven alkaloids from Corydalis Rhizoma, namely protopine (1), α-allocryptopine (2), tetrahydrocolumbamine (3), coptisine (4), palmatine (5), berberine (6), dehydrocorydaline (7), D,L-tetrahydropalmatine (8), tetrahydroberberine (9), corydaline (10) and tetrahydrocoptisine (11) were simultaneously quantified using a newly developed high performance liquid chromatography-diode array detector (HPLC-DAD) method. The influence of vinegar and wine processing on the content of the main alkaloids of Corydalis Rhizoma was investigated. For this purpose, two common formulations with clinical application, namely the water decoction of Corydalis Rhizoma and its formula Jin Ling Zi San (combination of Corydalis Rhizoma and Toosendan Fructus) were studied. In the two water decoctions, wine and vinegar processing increased the amount of tertiary alkaloids. The differences were more pronounced for Jin Ling Zi San, in which case the content of all tertiary alkaloids (compounds 1, 2, 3, 8, 9, 10, 11) was increased by wine processing.

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Qualitative and quantitative determination of ten alkaloids in traditional Chinese medicine Corydalis yanhusuo W.T. Wang by LC–MS/MS and LC–DAD

Cited by 101 publications

References 9 publications

A traditional Chinese medicine formulation consisting of Rhizoma Corydalis and Rhizoma Curcumae exerts synergistic anti-tumor activity

A traditional Chinese medicine formulation consisting of Rhizoma Corydalis and Rhizoma Curcumae exerts synergistic anti-tumor activity

Simultaneous determination of eleven alkaloids in Corydalis decumbens by HPLC with diode-array detection

Influence of Vinegar and Wine Processing on the Alkaloid Content and Composition of the Traditional Chinese Medicine Corydalis Rhizoma (Yanhusuo)

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