Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

Wei, Shihu; Zhang, Pei‐Cheng; Feng, Xizhi; Kodama, Hiroyuki; Chen, Yu; Chen, Guang

doi:10.1007/s11418-011-0560-8

Cited by 14 publications

(14 citation statements)

References 12 publications

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“…Phytochemical investigations have characterized the plant by the presence of a wide range of compounds, such as iridoids, secoiridoids, flavonoids, triterpenoids, alkaloids, and other types of secondary metabolites [6][7][8]. Some published methods have focused on the qualitative and quantitative determination of iridoids and secoiridoids in the plant by liquid chromatography or liquid chromatography-mass spectrometry [9,10]. Nevertheless, few of these methods have been aimed at determining the geographical origin of G. straminea by characteristic marker components.…”

Section: Introductionmentioning

confidence: 99%

Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses

et al. 2019

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The root of Gentiana straminea Maxim. (Gentianaceae), is officially listed as “Qin-Jiao” in the Chinese Pharmacopoeia for the treatment of rheumatic arthritis, icteric hepatitis, constipation, pain, and hypertension. To establish the geographical origin traceability in G. straminea, its chemical profiles were determined by a UPLC-Q exactive mass spectrometer, from which 43 compounds were identified by comparing retention times and mass spectrometry. Meanwhile, a pair of isomers (loganin and secologanol) was identified by mass spectrometry based on their fragmentation pathway. A total of 42 samples from difference habitats were determined by an UPLC-Q exactive mass spectrometer and the data were assayed with multivariate statistical analysis. Eight characteristic compounds were identified to determine the geographical origin of the herb. To estimate the key characteristic markers associated with pharmacological function, the inhibiting activities of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced macrophages were examined. This finding is crucial in realizing the determination of botanical origin and evaluating the quality of G. straminea.

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Section: Introductionmentioning

confidence: 99%

Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses

et al. 2019

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Section: Introductionmentioning

confidence: 99%

“…Gentiopicroside, loganic acid, swertiamarin and sweroside are normally used as the chemical references for quality criteria of Gentianae Macrophyllae Radix (Mustafa et al, 2015;Pan et al, 2015). Nevertheless, these iridoids are coexisting components in all of the Gentiana species (Jiang et al, 2005), and could not achieve the specific authentication of the genuine from the substitutes (Wei et al, 2012). In this paper, we performed the chemical profiling of iridoids and secoiridoids by liquid chromatography (LC)-electrospray ionization (ESI)-ion-trap mass spectrometry (MS n ) to discriminate the genuine from the substitute species of Gentianae Macrophyllae Radix, which was aided by quadrupole time-of-flight (QTOF)-MS analysis.…”

Section: Introductionmentioning

confidence: 99%

Authentication of Gentiana straminea Maxim. and its substitutes based on chemical profiling of iridoids using liquid chromatography with mass spectrometry

Zhao

et al. 2016

Biomedical Chromatography

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A liquid chromatography-electrospray ionization-ion-trap mass spectrometry (MS ) method was established and applied for authentication of Gentiana straminea from the four substitutes, G. tibetica, G. lhassica, G. waltonii and G. robusta, based on chemical profiling of the principal iridoid glucosides aided by a quadrupole time-of-flight mass spectrometry. The fragmentation pathways of the three representative iridoid glucosides, loganic acid, gentiopicroside and sweroside, were investigated by MS analysis in negative ion mode, which assisted the characterization of analogs detected in the chromatographic profiling of the tested Gentiana species. In total, 25 iridoids were identified or tentatively characterized from G. straminea and four substitutes, in which 7-O-(4''-O-glucosyl)coumaroyl-loganic acid and 7-O-coumaroyl-loganic acid are diagnostic in G. straminea and can serve as the proposed chemical markers to discriminate it from morphologically similar substitutes.

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“…To date, several analytical methods based on NIR, HPLC-UV, LC-MS and LC-NMR have been published for quality control of Gentiana species (Cao and Wang, 2010;Chuang et al 2014;Pan et al 2014;Wei et al 2012;Wolfender et al 1997). However, to the best of our knowledge no report is available on simultaneous determination of the six inconstituents (1, loganic acid; 2, swertiamarin; 3, mangiferin; 4, gentiopicroside; 5, sweroside; and 6, ferulic acid) in the four species (Fig.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method

Pan

Shen

Zhang

et al. 2014

Biomedical Chromatography

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Many species from genus Gentiana (Gentianaceae) have a long history of applications as folk medicines in the world. A simple rapid UPLC-UV-MS/MS method has been developed and validated for the simultaneous determination of six index constituents (gentiopicroside, swertiamarin, loganic acid, sweroside, mangiferin and ferulic acid) from the four ethnomedicines (G. rigescens Franch. ex Hemsl., G. rhodantha Franch. ex Hemsl., G. scabra Bunge and G. farreri Balf. f.). The UPLC analysis was performed on Shim-Pack XR-ODS III (150 × 2.0 mm, 2.2 µm). The mobile phase was consisted of acetonitrile-0.1% formic acid water using gradient elution. The wavelength 242 nm was chose for the four iridoids as well as mangiferin and 320 nm was set for ferulic acid. Mass spectrometry was applied for identification and quantification for analytes with low concentration. All the regression equations revealed a good linear relationship (R(2) > 0.9993). Accuracy and precision were all within the required limits. The chromatogram fingerprints analysis combined with principal component analysis showed the similarity values of the four species were <0.788 while the similarity values of G. scabra Bunge and G. rigescens Franch. ex Hemsl. were >0.993, which provided reasonable foundation for utilization and clinical application of the four ethnomedicines. This developed method appears to be a useful tool for quality control of the four ethnomedicines.

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Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

Cited by 14 publications

References 12 publications

Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses

Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses

Authentication of Gentiana straminea Maxim. and its substitutes based on chemical profiling of iridoids using liquid chromatography with mass spectrometry

Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method

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