Qualitative and quantitative determination by Raman spectroscopy of different sulphur oxoacid anions, existing in equilibrium in oxidized leach liquors, produced by desulphurization of flue gases

Milićev, S.; Stergaršek, Andrej

doi:10.1016/0584-8539(89)80128-x

Cited by 8 publications

(7 citation statements)

References 12 publications

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“…Main vibrational modes and related wavenumbers are labeled in inset from literature references. [82][83][84][85] In agreement with theoretical studies, [85] the experimental Raman spectrum of hypotaurine contains peculiar signals common to sulfur oxoacids and related to S═O/SÀO and C-S bonds. [82] Despite sharing S-related signals, hypotaurine and taurine present bold spectral differences according to the details of their bond structure.…”

Section: Antioxidant Molecules: Hypotaurine Taurine and Glutathionesupporting

confidence: 78%

“…[82][83][84][85] In agreement with theoretical studies, [85] the experimental Raman spectrum of hypotaurine contains peculiar signals common to sulfur oxoacids and related to S═O/SÀO and C-S bonds. [82] Despite sharing S-related signals, hypotaurine and taurine present bold spectral differences according to the details of their bond structure. [83] The Raman response recorded for sulfinic acid hypotaurine includes sharp signals at 973 and 985 cm À1 , which can be assigned to S═O stretching (cf.…”

Section: Antioxidant Molecules: Hypotaurine Taurine and Glutathionesupporting

confidence: 78%

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Raman spectroscopy in cell biology and microbiology

Pezzotti

2021

J Raman Spectroscopy

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The Raman spectrum of living cells and microorganisms contains highly specific fingerprint‐like signatures, useful in unequivocally identifying different species and interpreting physiological and metabolic responses to environmental stressors. In situ Raman imaging with dedicated highly sensitive instruments can translate selected spectroscopic fingerprints into vivid snapshots of molecular species or specific physiological reactions. Time‐lapse experiments, crucial in characterizing growth‐dependent phenomena and metabolic response to drugs or substrates, are possible because Raman imaging is life compatible. This review covers miscellaneous examples of Raman analyses and imaging of eukaryotic cells, bacteria, and viruses. Fundamental microbiological analyses covered here include (i) identification of different species of cells, bacteria, and viruses; (ii) characterization of the metabolic responses of cells and bacteria to different substrates; (iii) time‐lapse analyses of cell metabolic reactions upon viral inoculation; (iv) chemical imaging of axon sprouting in neuronal cells; and (v) visualization of the myelinating activity of living Schwann cells in coculture with neuronal cells. The spectroscopic findings displayed here, which are based on a machine learning approach applied to Raman analysis and imaging, demonstrate the invaluable potential for Raman spectroscopy in biophysics research.

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Section: Antioxidant Molecules: Hypotaurine Taurine and Glutathionesupporting

confidence: 78%

Section: Antioxidant Molecules: Hypotaurine Taurine and Glutathionesupporting

confidence: 78%

Raman spectroscopy in cell biology and microbiology

Pezzotti

2021

J Raman Spectroscopy

111

100

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“…Hypotaurine and its oxidation product taurine are both oxoacids of sulfur that play an important role as antioxidants and in regulating glucose-induced changes. Figure a,b show ball-and-stick models of the hypotaurine and taurine structures, respectively, with their principal vibrational modes and related frequencies labeled from specific literature (refs − ). The Raman spectrum of hypotaurine is shown in Figure a, as collected in the study on the pure compound.…”

Section: Resultsmentioning

confidence: 99%

“…In (a–c), ball-and-stick models of hypotaurine, taurine, and glutathione structures, respectively, with the principal vibrational modes and related frequencies from published literature. − …”

Section: Resultsmentioning

confidence: 99%

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Raman Probes forIn SituMolecular Analyses of Peripheral Nerve Myelination

Pezzotti

Adachi

Miyamoto

et al. 2020

ACS Chem. Neurosci.

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The myelinating activity of living Schwann cells in coculture with neuronal cells was examined in situ in a Raman microprobe spectroscope. The Raman label-free approach revealed vibrational fingerprints directly related to the activity of Schwann cells' metabolites and identified molecular species peculiar to myelinating cells. The identified chemical species included antioxidants, such as hypotaurine and glutathione, and compartmentalized water, in addition to sphingolipids, phospholipids, and nucleoside triphosphates also present in neuronal and nonmyelinating Schwann cells. Raman maps at specific frequencies could be collected, which clearly visualized the myelinating action of Schwann cells and located the demyelinated ones. An important finding was the spectroscopic visualization of confined water in the myelin structure, which exhibited a quite pronounced Raman signal at ∼3470 cm −1 . This peculiar signal, whose spatial location precisely corresponded to a low-frequency fingerprint of hypotaurine, was absent in unmyelinating cells and in bulk water. Raman enhancement was attributed to frustration in the hydrogen-bond network as induced by interactions with lipids in the myelin sheaths. According to a generally accepted morphological model of myelin, an explanation was offered of the peculiar Raman scattering of water confined in intraperiod lines, according to an ordered hydrogen bonding structure. The possibility of concurrently mapping antioxidant molecules and compartmentalized water structure with high spectral accuracy and microscopic spatial resolution enables probing myelinating activity and might play a key-role in future studies of neuronal pathologies. Compatible with life, Raman microprobe spectroscopy with the newly discovered probes could be suitable for developing advanced strategies in the reconstruction of injured nerves and nerve terminals at neuromuscular junctions.

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