Qualitative and quantitative analysis of uric acid, creatine and creatinine together with carbohydrates in biological material by HPTLC

Klaus, R.; Fischer, W.; Hauck, H. E.

doi:10.1007/bf02321427

Cited by 29 publications

(6 citation statements)

References 15 publications

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“…Due to the importance of creatinine in clinical research, a variety of analytical methods have been developed for detecting creatinine in urine, including Jaffe reaction spectrophotometric method [4], enzymatic method [5], capillary zone electrophoresis [6], high performance liquid chromatography (HPLC) [7], high performance thin-layer chromatography (HPTLC) [8], liquid chromatography tandem mass spectrometry (LC-MS) [9], gas chromatography mass spectrometry (GC-MS) [10], isotope dilution extractive electrospray ionization tandem mass spectrometry (ESI-MS) [11], Raman spectroscopy [12] and surface-enhanced Raman scattering (SERS) [13]. Compared to traditional analytical methods, Raman and SERS methods offer several advantages.…”

Section: Introductionmentioning

confidence: 99%

Reagent- and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)

Li¹,

Du²,

Zhao³

et al. 2015

Biomed. Opt. Express

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We report a novel reagent- and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 µg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, cost-effective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.

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Section: Introductionmentioning

confidence: 99%

Reagent- and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)

Li¹,

Du²,

Zhao³

et al. 2015

Biomed. Opt. Express

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“…It is one of the most widely used markers of renal function. Although in the clinical laboratory creatinine levels were commonly measured by Jaffé-based spectrophotometric method [2], the search for rapid analytical tools to measuring renal markers has prompted the development of various assays such as HPLC-UV [3][4][5][6][7] and HPLC-MS detection [8][9][10], HPTLC [11,12], CE [13][14][15][16][17], , and enzymatic methods [22,23]. HPLC and CE methods, without precolumn treatments, appear to be the simplest options for analysis of creatinine; in particular the use of CE for the biological fluids analysis in the clinical laboratory is an attractive alternative to many current analytical methods.…”

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confidence: 99%

Determination of creatinine in human serum by short‐end injection capillary zone electrophoresis

Zinellu

Carru

Usai³

et al. 2004

Electrophoresis

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A new ultra-rapid free-solution capillary zone electrophoresis method to measure serum creatinine is presented. Procedural parameters such as injection mode, concentration and pH of phosphate running buffer and acidic deproteinization of serum samples were investigated. Short-end injection permits a decrease of the analysis time by injecting samples at the outlet end of a silica capillary closest to the detection window, so reducing the migration distance. Thus, when a capillary with an effective length of 10.2 cm and a 40 mmol/L sodium phosphate buffer pH 2.35 was used, the obtained migration time of the creatinine peak was the shortest never described before, about 1.1 min. These conditions give a good reproducibility of the migration times (coefficient of variation, CV% < 0.5) and the peak areas (CV% < 2.8). Intra- and interassay CV were 3.06 and 6.26%, respectively, and analytical recovery was 99.4%. We compared our proposed method to Jaffé colorimetric assay, by measuring serum creatinine in 128 normal subjects. The obtained data were analyzed by the Passing and Bablok regression and Bland-Altman test. Creatinine concentration in healthy subjects was also used to investigate on its relationships with plasma thiols levels.

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“…Chromatographic properties of amino-silica gel layers are similar to those of nonmodified silica gel, and some identical solvent systems can be used with both sorbents ( Table 6). The main advantage of amino-bonded silica is that it affords simple detection of separated sugars by a thermal in situ reaction (56)(57)(58)(59). Sugars are readily converted, leading to stable, intensely fluorescing derivatives.…”

Section: Aminopropyl-bonded Silicamentioning

confidence: 99%

Carbohydrates

Maloney¹

2003

Handbook of Thin-Layer Chromatography

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Qualitative and quantitative analysis of uric acid, creatine and creatinine together with carbohydrates in biological material by HPTLC

Cited by 29 publications

References 15 publications

Reagent- and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)

Reagent- and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)

Determination of creatinine in human serum by short‐end injection capillary zone electrophoresis

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