Qualitative and quantitative analysis of steroidal saponins in crude extracts from Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis by high performance liquid chromatography coupled with mass spectrometry

Zhang, Ting; Liu, Hai; Liu, Xue-Ting; Xu, Defeng; Chen, Xiaoqing; Wang, Qiang

doi:10.1016/j.jpba.2009.08.020

Cited by 63 publications

(30 citation statements)

References 24 publications

(25 reference statements)

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“…Paris sp. is a perennial medicinal plant and is a promising candidate for the development of anti-cancer drugs [29–31], and is important forage plant as identified by the informants. Overall, wild forage plants consumed by mithuns were diverse and abundant in the study site.…”

Section: Resultsmentioning

confidence: 99%

Prioritizing fodder species based on traditional knowledge: a case study of mithun (Bos frontalis) in Dulongjiang area, Yunnan Province, Southwest China

Geng

Ranjitkar

et al. 2017

J Ethnobiology Ethnomedicine

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BackgroundLivestock rearing is one of the oldest and most important types of smallholder farming worldwide. The sustainability of livestock production depends on the efficient utilization of locally available resources. Some traditional methods of raising livestock may offer valuable lessons in this regard. This study documented and evaluated local knowledge of wild forage plants in the Dulongjiang area in Southwest China in the context of rearing mithun (Bos frontalis) in order to provide a sound evidence base for tree fodder selection and the establishment of integrated tree-crop-livestock systems.MethodsThe snowball technique was used to identify key informants with specific knowledge about the topic. Free listing and semi-structured interviews were conducted with 58 households. Participatory investigation and transit walks were used to investigate potential fodder species. Ethnobotanical information was collected, documented and organized.ResultsOverall, 142 wild forage plants from 58 families and 117 genera were identified. Species of the Poaceae, Rosaceae and Urticaceae families were most abundant, with 16, 14 and 11 species respectively identified as fodder plants. Our results indicated that tree/shrub forage plays a major role in the diet of mithun, unlike that of other ruminants. Mithun prefers to browse and move around the forest in search of food, particularly rough and even barbed leaves. Tree species like Debregeasia orientalis, Saurauia polyneura and Rubus species were identified as being important fodder sources. Farmers in this area have traditionally relied on common property resources such as community-managed forests and grasslands to feed their livestock. Farmers have strong incentive to raise mithuns rather than other livestock species due to Dulong people’s cultural preferences.ConclusionsThe wide variety of plants cited by the informants demonstrate the importance of traditional knowledge in gathering information about forage resources. This diversity also offers the prospect of identifying promising species which could be used as fodder plants. Identifying such species and tree fodder species in particular could help smallholder farmers to integrate trees, livestock and crops as part of a sustainable farming system.

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Section: Resultsmentioning

confidence: 99%

Prioritizing fodder species based on traditional knowledge: a case study of mithun (Bos frontalis) in Dulongjiang area, Yunnan Province, Southwest China

Geng

Ranjitkar

et al. 2017

J Ethnobiology Ethnomedicine

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“…Steroidal saponins can be divided into two types: furostanol glycosides, which bear an open side chain at C-22, and spirostanol glycosides, which contain a closed spiroketal ring at C-22. Based on abundant fragment ions generated by losing sugar chains, side chains and dehydration steroidal saponins were divided into types I to V according to the numbers of hydroxyl group and double bond in the A, B, C, and D rings [23, 24, 27, 30]. …”

Section: Resultsmentioning

confidence: 99%

“…All steroidal saponins produce abundant characteristic fragment ions through the loss of the branched glycoside chains and side chains or by dehydration; the ions could be rapidly identified by combining CFs with NLs at m/z 162 (C 6 H 10 O 5 ), 132 (C 5 H 8 O 4 ), 142 (C 8 H 14 O 2 ) and 18 (H 2 O). We used compounds purpureagitoside, timosaponin H1, and timosaponin AIII to discuss the fragmentation pathways in detail in the successive ESI–MS n experiments [5, 23, 24, 27, 30]. …”

Section: Resultsmentioning

confidence: 99%

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Rapid Screening of Chemical Constituents in Rhizoma Anemarrhenae by UPLC‐Q‐TOF/MS Combined with Data Postprocessing Techniques

Shan

Yuan

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Rhizoma Anemarrhenae, a famous traditional Chinese medicine (TCM), is the dried rhizome of Anemarrhena asphodeloides Bge. (Anemarrhena Bunge of Liliaceae). The medicine presents anti-inflammatory, antipyretic, sedative, and diuretic effects. The chemical constituents of Rhizoma Anemarrhenae are complex and diverse, mainly including steroidal saponins, flavonoids, phenylpropanoids, benzophenones, and alkaloids. In this study, UPLC-Q-TOF/MS was used in combination with data postprocessing techniques, including characteristic fragments filter and neutral loss filter, to rapidly classify and identify the five types of substances in Rhizoma Anemarrhenae. On the basis of numerous literature reviews and according to the corresponding characteristic fragments produced by different types of compounds in combination with neutral loss filtering, we summarized the fragmentation patterns of the main five types of compounds and successfully screened and identified 32 chemical constituents in Rhizoma Anemarrhenae. The components included 18 steroidal saponins, 6 flavonoids, 4 phenylpropanoids, 2 alkaloids, and 2 benzophenones. The method established in this study provided necessary data for the study on the pharmacological effects of Rhizoma Anemarrhenae and also provided the basis for the chemical analysis and quality control of TCMs to promote the development of a method for chemical research on TCMs.

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“…It appeared with the name ''ZaoXiu'', and many wellknown formulary Chinese Medicines contain Rhizoma Paridis, such as Yunnan Baiyao Powder and Gongxuening Capsules produced by Yunnan Baiyao Group Ltd. Co. (Yunnan, China), Loulian Capsules (Tonghua Wantong Pharmaceutical Ltd. Co., Tonghua, China), Jidesheng Sheyao Tablet (Nantong Jinghua Pharmaceutical Ltd. Co., Nantong, China). Rhizoma Paridis saponins (RPS) (Man et al, 2009a), as steroidal saponins, are the main and the active components in PPY (Liu et al, 2012;Qin et al, 2012;Zhang et al, 2010). They recently attracted particular interests because modern pharmacology research indicated these steroidal saponins appeared to exhibit a variety of biological activities, such as antitumor (Man et al, 2009b(Man et al, , 2011a, antifungal activity (Deng et al, 2008), inhibitory activities against abnormal uterine bleeding (Fu et al, 2008) and anthelmintic activity (Wang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Preparative separation and purification of steroidal saponins inParis polyphyllavar.yunnanensisby macroporous adsorption resins

Liu

Wang

Gao

et al. 2013

Pharmaceutical Biology

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Context: Saponins are active compounds in natural products. Many researchers have tried to find the method for knowing their concentration in herbs. Some methods, such as solid-liquid extraction and solvent extraction, have been developed. However, the extraction methods of the steroidal saponins from Paris polyphylla Smith var. yunnanensis (Liliaceae) are not fully researched. Objective: To establish a simple extraction method for the separation of steroidal saponins from the rhizomes of P. polyphylla Smith var. yunnanensis. Materials and methods: Macroporous adsorption resins were used for the separation of steroidal saponins. To select the most suitable resins, seven kinds of macroporous resins were selected in this study. The static adsorption and desorption tests on macroporous resins were determined. Also, we optimized the temperature and the ethanol concentration in the extraction method by the contents of five kinds of saponins. Then, we compared the extraction method with two other methods. Results: D101 resin demonstrated the best adsorption and desorption properties for steroidal saponins. Its adsorption data fits best to the Freundlich adsorption model. The contents of steroidal saponins in the product were 4.83-fold increased with recovery yields of 85.47%. Discussion and conclusion: The process achieved simple and effective enrichment and separation for steroidal saponins. The method provides a scientific basis for large-scale preparation of steroidal saponins from the Rhizoma Paridis and other plants.

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Qualitative and quantitative analysis of steroidal saponins in crude extracts from Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis by high performance liquid chromatography coupled with mass spectrometry

Cited by 63 publications

References 24 publications

Prioritizing fodder species based on traditional knowledge: a case study of mithun (Bos frontalis) in Dulongjiang area, Yunnan Province, Southwest China

Prioritizing fodder species based on traditional knowledge: a case study of mithun (Bos frontalis) in Dulongjiang area, Yunnan Province, Southwest China

Rapid Screening of Chemical Constituents in Rhizoma Anemarrhenae by UPLC‐Q‐TOF/MS Combined with Data Postprocessing Techniques

Preparative separation and purification of steroidal saponins inParis polyphyllavar.yunnanensisby macroporous adsorption resins

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