Qualitative and Quantitative Analysis of 24 Components in Jinlianhua Decoction by UPLC–MS/MS

Fang, Ming-Yue; Liu, Shuangyue; Wang, Qingqing; Gu, Xuan; Ding, Pengmin; Ding, Yi; Liu, Junxiu; Wang, Rufeng

doi:10.1007/s10337-019-03806-w

Cited by 14 publications

(10 citation statements)

References 22 publications

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“…## P < 0.01, ### P < 0.001: significantly differently from the Normal group; *P < 0.05, **P < 0.01, ***P < 0.001: significantly differently from the Infected group. (Fang et al, 2019). Rosmarinic acid has been reported to exert inhibitory activity against NA (Mahalapbutr et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Exploring the active ingredients and pharmacological mechanisms of the oral intake formula Huoxiang Suling Shuanghua Decoction on influenza virus type A based on network pharmacology and experimental exploration

Tang

Wang²,

Zhang³

et al. 2022

Front. Microbiol.

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ObjectiveTo investigate the active ingredients, underlying anti-influenza virus effects, and mechanisms of Huoxiang Suling Shuanghua Decoction (HSSD).Materials and methodsThe therapeutic effect of HSSD were confirmed through the survival rate experiment of H1N1-infected mice. Then, the HSSD solution and the ingredients absorbed into the blood after treatment with HSSD in rats were identified by UPLC/Q-TOF MS, while the main contents of ingredients were detected by high performance liquid chromatography (HPLC). Next, a systems pharmacology approach incorporating target prediction, gene ontology (GO) enrichment, kyoto encyclopedia of genes and genomes (KEGG) pathway analysis, and molecular docking were performed to screen out the active compounds and critical pathways of HSSD in treating influenza. According to prediction results, real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry assay were used to detect the mRNA and protein expression levels of critical targets in H1N1-infected mice lungs.ResultsHuoxiang Suling Shuanghua Decoction improved the survival rate of H1N1-infected mice and prolonged the mice’s lifespan. Besides, HSSD exerts an antivirus effect by decreasing the levels of hemagglutinin (HA) and nucleoprotein (NP) to inhibit the replication and proliferation of H1N1, reducing the lung pathological state, inhibiting the cell apoptosis in the lung, and regulating the abnormal responses of peripheral blood, including GRA, LYM, white blood cell (WBC), PLT, and hemoglobin (HGB). Then, 87 compounds in the HSSD solution and 20 ingredients absorbed into the blood after treatment with HSSD were identified. Based on this, combined with the network analysis and previous research on antivirus, 16 compounds were screened out as the active components. Moreover, 16 potential targets were predicted by network pharmacology analysis. Next, molecular docking results showed stable binding modes between compounds and targets. Furthermore, experimental validation results indicated that HSSD regulates the contents of Immunoglobulin A (IgA), Immunoglobulin M (IgM), and Immunoglobulin G (IgG) in serum, modulating the levels of IFN-γ, IL-6, IL-10, MCP-1, MIP-1α, and IP-10 in the lung tissue, and significantly decreasing the mRNA and protein expressions of TLR4, CD14, MyD88, NF-κB p65, HIF1 α, VEGF, IL17A, and IL6 in the lung tissue.ConclusionHuoxiang Suling Shuanghua Decoction exerts an anti-influenza effect by affecting the expressions of mRNA and protein including TLR4, CD14, MyD88, NF-kB p65, HIF-1α, VEGF, IL17A, IL6, and inhibiting the accumulation of inflammation. Our study provided experimental pieces of evidence about the practical application of HSSD in treating influenza.

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Section: Discussionmentioning

confidence: 99%

Exploring the active ingredients and pharmacological mechanisms of the oral intake formula Huoxiang Suling Shuanghua Decoction on influenza virus type A based on network pharmacology and experimental exploration

Tang

Wang²,

Zhang³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In order to study the difference of metabolomics between the two cultivars at different developmental stages, untargeted metabolomics was carried out using a previously described method [20]. The freeze-dried samples were crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz.…”

Section: Lc-ms and Lc-ms/ms Analysismentioning

confidence: 99%

Title: Transcriptome and metabolome analyses reveal pathways associated with fruit color in plum (Prunus salicina Lindl.)

Chen

Wang

Cui

et al. 2021

Preprint

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Background In order to reveal the mechanism of fruit color changes in plum, two common plum cultivars Changli84 (Ch84, red fruit) and Dahuangganhe (D, yellow fruit) in Northeast China were selected as plant materials. Transcriptome sequencing and metabonomic analyzing were performed at three different developmental stages: young fruit stage, colour-change stage, and maturation stage. Results “Flavonoid biosynthesis” was significantly enriched in the KEGG analysis. Some DEGs in “Flavonoid biosynthesis” pathway had an opposite trend between the two cultivars, such as CHS, DFR and FLS. In the current study, procyanidin B1 and B2 had the highest level at young fruit stage in Ch84 and the content of procyanidin B2 decreased sharply at the color change stage. Conversely, the content of cyanidin increased with the growth of fruit and reached the peak at the maturation stage. Conclusion The content of procyanidin B1 and B2 in plums at young fruit stage might be the leading factors of the matured fruit color. At the maturation stage, the cyanidin produced by procyanidins keeps the color of the fruit red. Correspondingly, genes in “flavonoid biosynthesis” pathway play critical roles in regulating the accumulation of anthocyanin in plum.

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“…In order to study the difference of metabolomics between the two cultivars at different developmental stages, untargeted metabolomics was carried out using a previously described method [24]. The freeze-dried samples were crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz.…”

Section: Lc-ms and Lc-ms/ms Analysismentioning

confidence: 99%

Transcriptome and metabolome analyses reveal pathways associated with fruit color in plum (Prunus salicina Lindl.)

Chen

Wang

Cui

et al. 2021

Preprint

View full text Add to dashboard Cite

Background In order to reveal the mechanism of fruit color changes in plum, two common plum cultivars Changli84 (Ch84, red fruit) and Dahuangganhe (D, yellow fruit) in Northeast China were selected as plant materials. Transcriptome sequencing and metabonomic analyzing were performed at three different developmental stages: young fruit stage, colour-change stage, and maturation stage. Results “Flavonoid biosynthesis” was significantly enriched in the KEGG analysis. Some DEGs in “Flavonoid biosynthesis” pathway had an opposite trend between the two cultivars, such as CHS , DFR and FLS . Also, transcriptional control of MBW (MYB–bHLH–WD) protein complexes showed a close relationship with plum fruit color, especially the expression of MYBs and bHLHs . In the current study, procyanidin B1 and B2 had the highest level at young fruit stage in Ch84 and the content of procyanidin B2 decreased sharply at the color change stage. Conversely, the content of cyanidin increased with the growth of fruit and reached the peak at the maturation stage. Conclusion The content of procyanidin B1 and B2 in plums at young fruit stage might be the leading factors of the matured fruit color. At the maturation stage, the cyanidin produced by procyanidins keeps the color of the fruit red. Correspondingly, genes in “flavonoid biosynthesis” pathway play critical roles in regulating the accumulation of anthocyanin in plum.

show abstract

Qualitative and Quantitative Analysis of 24 Components in Jinlianhua Decoction by UPLC–MS/MS

Cited by 14 publications

References 22 publications

Exploring the active ingredients and pharmacological mechanisms of the oral intake formula Huoxiang Suling Shuanghua Decoction on influenza virus type A based on network pharmacology and experimental exploration

Exploring the active ingredients and pharmacological mechanisms of the oral intake formula Huoxiang Suling Shuanghua Decoction on influenza virus type A based on network pharmacology and experimental exploration

Title: Transcriptome and metabolome analyses reveal pathways associated with fruit color in plum (Prunus salicina Lindl.)

Transcriptome and metabolome analyses reveal pathways associated with fruit color in plum (Prunus salicina Lindl.)

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