Qualitative and Molecular Screening of Potential Ligninolytic Microbes from Termite (Coptotermes curvignathus) Gut

Simol, Carlina Freddie; Chubo, John Keen; King, Patricia Jie Hung; Ong, Kian Huat; Chew, Cindy; Nawi, Khalid

doi:10.33736/bjrst.2879.2021

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…But Hameed et al [53] suggested that combining conventional and molecular techniques can help achieve the accuracy, rapidness, and reliability of microbiome results. Hence, the technique also been proven in a few groups of wildlife studies throughout Southeast Asia [23,29,33,51,54,55].…”

Section: Methodological Variability In Gut Microbiome Studies In Sout...mentioning

confidence: 99%

“…Minard et al [20], Surat et al [21], and Rosso et al [22]) and honeybees (eg. Lombogia et al [23], Duong et al [24], Gruneck et al [25], and Lanh et al [26]), there are also a study on fleas by Rombot et al [27] Badrulisham et al [28], termites by Simol et al [29], cockroach by Ni'matuzahroh et al [30] and weevil by Farah-Nadia et al [31]. Following closely, mammals are a prominent focus with 19 studies.…”

Section: Summary Of Microbiome Studies In Southeast Asiamentioning

confidence: 99%

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Assessing Methodological Variability in Gut Microbiome Studies: Lessons from Southeast Asian for Effective Conservation Strategies

Haris,

Sariyati,

Ramli

et al. 2024

BIO Web Conf.

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Gut microbiome studies have gained significant attention in recent years due to their potential in unveiling the role of microbial communities in animals’ health and ecological processes. However, the lack of standardized protocols in sample handling and processing across studies introduces variability, impeding the comparability of findings. This study addresses this issue by examining methodological variations in gut microbiome research on wildlife and domesticated animals in Southeast Asia. A comprehensive search of 91 relevant studies on the SCOPUS database yielded 54 suitable publications for review, encompassing diverse taxa such as invertebrates (20), fishes (7), reptiles (3), birds (5), and mammals (19). Notably, various methodological approaches were employed to characterize microbial communities, including the source of isolation, various culture-based approaches, sequencing methods, and the targeted markers. Based on the information provided in this study, future studies should strive to develop guidelines and best practices specific to gut microbiome studies. This would enhance comparability and facilitate the integration of findings. Such efforts will also advance our understanding of the microbial diversity associated with wildlife, and its potential implications for their health and conservation.

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Section: Methodological Variability In Gut Microbiome Studies In Sout...mentioning

confidence: 99%

Section: Summary Of Microbiome Studies In Southeast Asiamentioning

confidence: 99%

Assessing Methodological Variability in Gut Microbiome Studies: Lessons from Southeast Asian for Effective Conservation Strategies

Haris,

Sariyati,

Ramli

et al. 2024

BIO Web Conf.

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“…21 Microbes found in the insect gut, including Pseudomonas, Bacillus, Enterobacter, and Paenibacillus, are known to produce lignocellulosic enzymes. 22 Complex interactions exist between gut microbes and their insect hosts to the benefit of both parties. 8 Cellulolytic bacterial isolates were previously reported in the gut of the grasshoppers Oxya chinensis and Oxya velox.…”

Section: Introductionmentioning

confidence: 99%

Grasshopper (Oxya chinensis) Gut Bacteria and their Cellulolytic Activity

Munir,

Sitinjak,

Nasution

2024

J. Pure Appl. Microbiol.

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Lignocellulose is a core component of plant biomass and the most abundant carbohydrate polymer in nature. It is cheap and renewable and has several potential applications; however, it remains underutilized because of its recalcitrance to degradation. Cellulolytic microbes have been found in the gut of herbivorous insects, such as grasshoppers. This study aimed to isolate lignocellulolytic bacteria from the gut of grasshoppers (Oxya chinensis) and determine their diversity and potential biomass-degrading activity. A total of 27 culturable isolates were obtained from the grasshopper foregut, midgut, and hindgut. The bacteria hydrolyzed cellulose and lignin, as indicated by a cellulolytic index of 0.12–1.23 and ligninolytic index of 0.1–1.47. Five potential cellulolytic bacterial isolates were selected. Based on 16S rRNA sequencing, the isolates were identified as Bacillus wiedmannii (foregut), Bacillus marcorestinctum, Bacillus halotolerans (midgut), Paenibacillus zanthoxyli, and Bacillus hominis (hindgut). The highest specific cellulolytic activity (0.0068 U/mg) was detected in B. wiedmannii (OCF2), which could be exploited as a potential source of cellulases.

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“…Our previous screening of 27 termite (Coptotermes curvignathus) gut isolates obtained from the collection of the Microbiology Laboratory, Faculty of Agricultural Science and Forestry, Universiti Putra Malaysia Bintulu Sarawak Campus (UPMKB), Malaysia detected only 3 isolates with ligninolytic activity, based on 4 different ligninolytic indicator dyes [16]. The 16S rRNA gene sequencing of these isolates identified them as Bacillus toyonensis, Bacillus cereus, and Bacillus thuringiensis as indicated in Table S1.…”

Section: Introductionmentioning

confidence: 99%

Enhancing plant nutrient availability in composted paddy husk using Bacillus spp. isolated from termite (Coptotermes curvignathus) gut

Simol¹,

Chubo²,

King³

et al. 2022

ScienceAsia

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Paddy husk (PH) is a waste item generated from rice production that can be used as an organic fertilizer through composting. High lignin content is an issue with PH composting as it impedes the production of high-quality organic fertilizer. Improvements to the composting process can enhance the agronomic properties of compost produced from PH. The objectives of this study were to: (i) determine the ability of Bacillus spp. in enhancing the decomposition of PH and (ii) determine the ability of Bacillus spp. in increasing the macronutrient content of composted PH. Different ligninolytic active Bacillus spp. from termite gut (either singly or a cocktail) were added to 7 of 9 compost boxes containing PH compost mixtures and were allowed to decompose for a period of 60 days. Each treatment was represented by 3 samples, and the compost boxes were arranged in a completely randomized design (CRD) with 3 replications. Results showed that the addition of Bacillus spp. promoted the production of matured compost within 60 days with significantly higher amounts of phosphorus, potassium, calcium, and magnesium. Germination index (GI) of all composted PH added with Bacillus spp. ranged from 82.51 to 95.83%, suggesting that composted PH has lower phytotoxicity than compost without Bacillus spp. isolate. In general, addition of Bacillus spp. to PH waste promoted the production of PH composts with improved macronutrient availability and lower phytotoxicity levels.

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Qualitative and Molecular Screening of Potential Ligninolytic Microbes from Termite (Coptotermes curvignathus) Gut

Cited by 6 publications

References 27 publications

Assessing Methodological Variability in Gut Microbiome Studies: Lessons from Southeast Asian for Effective Conservation Strategies

Assessing Methodological Variability in Gut Microbiome Studies: Lessons from Southeast Asian for Effective Conservation Strategies

Grasshopper (Oxya chinensis) Gut Bacteria and their Cellulolytic Activity

Enhancing plant nutrient availability in composted paddy husk using Bacillus spp. isolated from termite (Coptotermes curvignathus) gut

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