Qualitative analysis of re mineralized carious lesions subjected to fluoride supplement through confocal laser scanning microscope

Abstract

Aim: 1] Comparative evaluation of the linear depth of induced remineralized lesions after subjecting to fluoride supplements and 2] To assess the average fluorescence at both the demineralized and the remineralized zones in all the three study groups under confocal laser scanning microscope. Method: Forty five sound human premolars extracted for orthodontic reasons were decoronated 1 mm below the cemento-enamel junction and coated with nail varnish except for a 3 × 3 mm window on the buccal surface. The samples were placed in 50 ml of de mineralizing solution at pH 4.6 for 96 hours. Following demineralization, the lower half of the 3 × 3 mm window in all the samples were covered with nail varnish to serve as control. The samples were randomly divided into three groups of fifteen teeth each (n = 15) and specimens in group A[Nfd] were remineralized using non-fluoridated dentifrice [control], those in groups B [Fd5] and group C [Fd10] using 500 ppm and 1000 ppm of fluoride containing dentifrice, respectively. The specimens were subjected to a 20 day remineralization treatment regimen and were sectioned into 100 µm thick sections and two images were captured on the buccal surface from either side of the midpoint of occluso-cervical length using confocal laser scanning microscope [CLSM]. Results: were tabulated and statistically analyzed by Anova. Study concluded that 1000 ppm fluoridated dentifrice showed a greater degree of remineralization than other groups and confocal laser scanning microscopes gives promising results in the diagnosis of early enamel lesions over the conventional methods.

Evaluation and Comparison of Self-applied Remineralizing Agents Using Confocal Microscopy: An In Vitro Study

“…The images were captured from the buccal surface that is one each from either side of the midpoint measured from the occluso-cervical length of the tooth at (5×) magnification and Argon laser was used at 488 nm wavelength for excitation and emission range of 498–514 nm. Using the Leica TCS SL in-built software, 3 the captured images were calibrated for the linear depth of fluorescence and the average/total fluorescence of the lesion. These values were noted, tabulated, and were compared with the evaluated demineralized values of the same specimen and were subjected to statistical analysis ( Figs 6 to 11 ).…”