Phytochemicals - Source of Antioxidants and Role in Disease Prevention 2018
DOI: 10.5772/intechopen.77365
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Qualitative Analysis of Phytochemicals from Sea Buckthorn and Gooseberry

Abstract: This chapter describes in detail recent research results obtained from the qualitative screening of different phytochemicals found in aqueous extracts of sea buckthorn and gooseberry, fruits with important pharmacological effects due to their high content in vitamin C. Phytochemical investigations reveal the presence of active principles (e.g., saponins, flavonoids, alkaloids, carbohydrates, terpenoids, etc.) in sea buckthorn and gooseberry and are accomplished by using well-established standard methods. All t… Show more

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Cited by 12 publications
(10 citation statements)
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“…Quercetin, gallic, salycilic, and ferulic acid solutions (4 mkg in every spot) were used as a reference. Plates were developed in 2% solutions of aluminum chloride, iron chloride, and phosphotungstic acid in ethanol (Sorescu et al, 2018;Shaikh and Patil, 2020). For a quantitative analysis of chemical constituents 120 mg of bark were poured into 1.5 mL of ethanol and extracted at 55 • C in an ultrasound chamber (Grad 80-35, Proton LLC, Moscow, Russia) for 35 min.…”
Section: Analytical Proceduresmentioning
confidence: 99%
“…Quercetin, gallic, salycilic, and ferulic acid solutions (4 mkg in every spot) were used as a reference. Plates were developed in 2% solutions of aluminum chloride, iron chloride, and phosphotungstic acid in ethanol (Sorescu et al, 2018;Shaikh and Patil, 2020). For a quantitative analysis of chemical constituents 120 mg of bark were poured into 1.5 mL of ethanol and extracted at 55 • C in an ultrasound chamber (Grad 80-35, Proton LLC, Moscow, Russia) for 35 min.…”
Section: Analytical Proceduresmentioning
confidence: 99%
“…Qualitative and quantitative phytochemical screening of the extract was carried out using the Standard methods described by [12,13]. The procedure for the determination of each phytochemical is elaborated below.…”
Section: Phytochemical Analysismentioning
confidence: 99%
“…For spot plate I, the result is positive if the white color is formed. Meanwhile, the brown color indicates a positive test result for spot plate II [23].…”
Section: A Alkaloidsmentioning
confidence: 99%
“…Meanwhile, the test tube was heated for 10 min for the tannin test, and 5 mL of FeCl 3 solution was added. If the sample contains tannin, the solution will turn to dark blue color [23].…”
Section: B Saponin and Tanninmentioning
confidence: 99%
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