Qualitation and Quantitation on Microplasma Jet for Bacteria Inactivation

Du, Changming; Ya, Liu; Huang, Yani; Li, ZiMing; Men, Rui; Men, Yue; Tang, Jun

doi:10.1038/srep18838

Cited by 14 publications

(17 citation statements)

References 37 publications

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“…Furthermore, our data indicate that the direct contribution of plasma-generated UV to the bactericidal effect and DNA fragmentation is negligible. This is in agreement with results derived from a different LTP source 42 . This does not exclude a possible contribution from UV radiation generated by the plasma in the formation of reactive oxygen species in the gas phase 43 .…”

Section: Discussionsupporting

confidence: 92%

Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level

Privat-Maldonado

O’Connell

Welch

et al. 2016

Sci Rep

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Low temperature plasmas (LTPs) generate a cocktail of reactive nitrogen and oxygen species (RNOS) with bactericidal activity. The RNOS however are spatially unevenly distributed in the plasma. Here we test the hypothesis that this distribution will affect the mechanisms underpinning plasma bactericidal activity focussing on the level of DNA damage in situ. For the first time, a quantitative, single cell approach was applied to assess the level of DNA damage in bacteria as a function of the radial distance from the centre of the plasma jet. Salmonella enterica on a solid, dry surface was treated with two types of LTP: an atmospheric-pressure dielectric barrier discharge plasma jet (charged and neutral species) and a radio-frequency atmospheric-pressure plasma jet (neutral species). In both cases, there was an inverse correlation between the degree of DNA damage and the radial distance from the centre of the plasma, with the highest DNA damage occurring directly under the plasma. This trend was also observed with Staphylococcus aureus. LTP-generated UV radiation was eliminated as a contributing factor. Thus valuable mechanistic information can be obtained from assays on biological material, which can inform the development of LTP as a complementary or alternative therapy for (topical) bacterial infections.

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Section: Discussionsupporting

confidence: 92%

Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level

Privat-Maldonado

O’Connell

Welch

et al. 2016

Sci Rep

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“…The measurements [30] show changes in the concentration of the dye and viable cells by analyzing the ultraviolet spectral spectrum by means of the spectral analysis device for different exposure times. The dye degradation and the microbes elimination due to the dramatic inhibitory effect of the survival microbes ratio can be explained by the fact that ultraviolet radiation, which is emitted from the plasma jet generated by the torch because of very high plasma temperatures and thus high intensity of ultraviolet radiation, and is also due to the excitation and ionization of water molecules by active electrons as shown in the following equations [31]: Figure 9 represents the sharp decrease in dye concentration as the exposure time increases. The decrease is stronger for direct exposure than for indirect exposure.…”

Section: Plasma Torchmentioning

confidence: 99%

Fast inactivation of microbes and degradation of organic compounds dissolved in water by thermal plasma

Galaly¹,

Oost²

2018

Plasma Sci. Technol.

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The multifunctionality and the advantages of thermal plasma for the fast inactivation of viable cells and degradation of organic compounds dissolved in waste water are presented. A complete bacterial inactivation process was observed and studied using a thermal plasma treatment source with very short application times, in particular for Staphylococcus aureus bundle spore survival. The survival curves and analyses of the experimental data of the initial and final densities of S. aureus bacteria show a dramatic inhibitory effect of the plasma discharge on the residual bacteria survival ratio. As the exposure time increased, the inactivation process rate increased for direct exposure more than it did for indirect exposure. The evaluation of direct and indirect exposure was based on the analysis of the ultraviolet spectrum from the absorbance spectra of the organic compound dye called benzene sulfonate (C 16 H 11 N 2 NaO 4 S) and of viable cells called S. aureus. Organic compounds were degraded and viable cells were killed in a short time by thermal plasma. Moreover, analyses of total carbon, total organic carbon, and total inorganic carbon showed a fast decrease in organically bound carbon, however, this was not as fast as the absorbance spectra revealed by the exposure time increasing more for direct exposure than indirect exposure. After 100 s of exposure to the organic compound dye the removal had a maximun of 40% for samples with indirect exposure to the plasma and a maximum of 90% for samples with the direct exposure. For both samples, where some organic contaminants still remained in treated water, four electrolytes (KCl, NaCl, Na 2 SO 4 , and CH 3 COONa) were added to be effective for complete sterilization, reaching a purity of 100%. A proposal is made for an optimized thermal plasma water purification system (TPWPS) to improve fast inactivation of microbes and the degradation of organic compounds dissolved in water (especially for direct exposure rather than indirect exposure) using a hybrid plasma torch with an electrical power of 125 kW (500 V-250 A) producing a high-temperature (10 000 K-19 000 K) plasma jet with a maximum gas consumption of 28 mg s −1 .

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“…Nonthermal plasma is an alternative to conventional sterilization methods [5] and is considered as a new sterilization technique in the conservation of materials from bacteria [4]. This method has several advantages such as the possibility of inactivating the bacteria at low temperatures (near room temperature), being economical, absence of residual toxicity, and appropriateness for applications when product conservation is required [6,7]. They have become very attractive for biomedical applications such as bacteria inactivation [8,9], wound healing [10], decontamination of medical equipment [11], and blood coagulation [12].…”

Section: Introductionmentioning

confidence: 99%

Studying the non-thermal plasma jet characteristics and application on bacterial decontamination

Al-rawaf

Fuliful

Khalaf³

et al. 2018

J Theor Appl Phys

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Non-thermal atmospheric-pressure plasma jet represents an excellent approach for the decontamination of bacteria. In this paper, we want to improve and characterize a non-thermal plasma jet to employ it in processes of sterilization. The electrical characteristics was studied to describe the discharge of the plasma jet and the development of plasma plume has been characterized as a function of helium flow rate. Optical emission spectroscopy was employed to detect the active species inside the plasma plume. The inactivation efficiency of non-thermal plasma jet was evaluated against Staphylococcus aureus bacteria by measuring the diameter of inhibition zone and the number of surviving cells. The results presented that the plasma plume temperature was lower than 34 C at a flow rate of 4 slm, which will not cause damage to living tissues. The diameter of inhibition zone is directly extended with increased exposure time. We confirmed that the inactivation mechanism was unaffected by UV irradiation. In addition, we concluded that the major reasons for the inactivation process of bacteria is because of the action of the reactive oxygen and nitrogen species which formed from ambient air, while the charged particles played a minor role in the inactivation process.

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Qualitation and Quantitation on Microplasma Jet for Bacteria Inactivation

Cited by 14 publications

References 37 publications

Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level

Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level

Fast inactivation of microbes and degradation of organic compounds dissolved in water by thermal plasma

Studying the non-thermal plasma jet characteristics and application on bacterial decontamination

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