Qualification of the B and W Mark B fuel assembly for high burnup. Third semi-annual progress report, July-December 1979

Coleman, T.A.; Coppola, E.J.; Doss, P.L.; Uotinen, V.O.; Davis, H.H.

doi:10.2172/6869098

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“…For simultaneous visualization of live and dead cells, the Zeiss 487909 filter combination (450-490 nm excitation, 510 nm dichroic and > 520 nm emission filter) was used. DAPI staining of cells was performed according to Coleman (1980) and the stained cells were examined using the Zeiss 487901 filter combination (365 nm exitation, 395 nm dichroic and >397 nm emission filter), which allowed the simultaneous detection of the blue fluorescence from DAPI-stained chlorotic (non-autofluorescent) cells and the red autofluorescence of fully pigmented cells. into proteins was quantified by determining the radioactivity from a 10 pl aliquot after precipitating proteins with 10% TCA.…”

Section: Methodsmentioning

confidence: 99%

Nitrogen-starvation-induced chlorosis in Synechococcus PCC 7942: adaptation to long-term survival

Görl¹,

Sauer²,

Baier³

et al. 1998

Microbiology

137

110

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When deprived of essential nutrients, the non-diazotrophic cyanobacterium Synechococcus sp. strain PCC 7942 undergoes a proteolytic degradation of the phycobiliproteins, its major light-harvesting pigments. This process is known as chlorosis. This paper presents evidence that the degradation of phycobiliproteins is part of an acclimation process in which growing cells differentiate into non-pigmented cells able to endure long periods of starvation. The time course of degradation processes differs for various photosynthetic pigments, for photosystem I and photosystem II activities and is strongly influenced by the illumination and by the experimental conditions of nutrient deprivation. Under standard experimental conditions of combined nitrogen deprivation, three phases of the differentiation process can be defined. The first phase corresponds to the well-known phycobiliprotein degradation, in phase 2 the cells lose chlorophyll a prior to entering phase 3, the fully differentiated state, in which the cells are still able to regenerate pigmentation after the addition of nitrate to the culture. An analysis of the protein synthesis patterns by two-dimensional gel electrophoresis during nitrogen starvation indicates extensive differential gene expression, suggesting the operation of tight regulatory mechanisms.

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Section: Methodsmentioning

confidence: 99%

Nitrogen-starvation-induced chlorosis in Synechococcus PCC 7942: adaptation to long-term survival

Görl¹,

Sauer²,

Baier³

et al. 1998

Microbiology

137

110

View full text Add to dashboard Cite

show abstract

Qualification of the B and W Mark B fuel assembly for high burnup. Third semi-annual progress report, July-December 1979

Abstract: Baboock & Wilcox CONTENTS. . Moderator aqd Doppler Reaf.ti.vity Coefficients for Equilibrium Cycles .

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Nitrogen-starvation-induced chlorosis in Synechococcus PCC 7942: adaptation to long-term survival

Nitrogen-starvation-induced chlorosis in Synechococcus PCC 7942: adaptation to long-term survival

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