QUALIDADE FISIOLÓGICA DE SEMENTES DE &lt;i&gt;Tabernaemontana fuchsiaefolia&lt;/i&gt; A. DC EM FUNÇÃO DO TESTE DE ENVELHECIMENTO ACELERADO¹

Moraes, Carlos Eduardo; Lopes, José Carlos; Farias, Carla Caroline Magalhães; Maciel, Khétrin Silva

doi:10.5902/1980509821114

Cited by 16 publications

(22 citation statements)

References 5 publications

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“…When the deterioration of the seeds increased, the speed of the germination reduced. These results were observed for Melanoxylon brauna and Tabernae montana (MORAES et al, 2016).…”

Section: Discussionsupporting

confidence: 68%

PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS

Flores

Ataíde

Borges³

et al. 2018

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In order to ensure the ex-situ conservation of a species, knowing the behavior of the seed during storage is necessary, as it is influenced by several factors inherent to the seed and by characteristics of the environment, such as temperature and relative humidity. This study aimed to evaluate the physiological and biochemical alterations on Cedrela fissilis (pink cedar) seeds during storage under different environmental conditions. Seeds were stored at 20ºC in desiccators containing salts that established the relative humidity of 34 %, 55 %, 75 %, and 93 %. Analysis were performed on fresh seeds (without storage -time zero), which were used as control, and every two months through a period of eight months of storage. At each assessment, the following aspects were quantified: water content; lipid peroxidation; and the activities of catalase and glucose-6-phosphate dehydrogenase. Germination and electrical conductivity tests were analysed. Seed vigor decreased on humidity at 20 °C. It correlated mainly with decrease of glucose-6-phosphate dehydrogenase activity and increase of lipid peroxidation. Keywords: Pink cedar, germination, vigor, enzymes, lipids. ResumoAlterações fisiológicas e bioquímicas durante o armazenamento de sementes de Cedrela fissilis Vellozo. Para garantir a conservação ex-situ de uma espécie, é necessário conhecer o comportamento das sementes durante o armazenamento, visto que este é influenciado por vários fatores inerentes à semente e às características do ambiente, tais como temperatura e umidade relativa. Este estudo teve como objetivo avaliar as alterações fisiológicas e bioquímicas em sementes de Cedrela fissilis (cedro rosa) durante o armazenamento sob diferentes condições ambientais. As sementes foram armazenadas a 20ºC em dessecadores contendo sais que estabeleceram as umidades relativas do ar de 34 %, 55 %, 75 % e 93 %. As análises foram realizadas em sementes frescas (sem armazenamento -tempo zero), usadas como controle, e a cada dois meses durante um período de oito meses de armazenamento. Em cada avaliação, foram quantificados: teor de água; peroxidação de lípidos; e as atividades das enzimas catalase e glicose-6-fosfato desidrogenase. Também, foi analisada a germinação e condutividade elétrica das sementes. O vigor das sementes diminuiu em todas as umidades a 20 °C, correlacionando-se, principalmente, com a diminuição na atividade de glicose-6-fosfato desidrogenase e com o aumento na peroxidação lipídica. Palavras-chave: Cedro rosa, germinação, vigor, enzimas, lipídios.

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“…When the deterioration of the seeds increased, the speed of the germination reduced. These results were observed for Melanoxylon brauna and Tabernae montana (MORAES et al, 2016).…”

Section: Discussionsupporting

confidence: 68%

PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS

Flores

Ataíde

Borges³

et al. 2018

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“…Embora alguns trabalhos de pesquisa tenham obtido sucesso com o uso da temperatura de 45 ºC para o envelhecimento acelerado de espécies florestais (SANTOS & PAULA, 2009;MORAES et al, 2016), sabe-se que as temperaturas utilizadas neste teste podem ser prejudiciais, dependendo do período de duração do mesmo. Segundo WATERWORTH et al (2016) as altas temperaturas e umidades são os principais fatores envolvidos no processo de envelhecimento natural das sementes, que podem causar danos oxidativos, resultando na deterioração de proteínas, do DNA e de estruturas celulares (WATERWORTH et al, 2015), o que pode levar a perda total da viabilidade da semente.…”

Section: Resultsunclassified

“…Vários trabalhos indicam o uso do teste de envelhecimento acelerado para avaliação do potencial fisiológico de sementes de espécies florestais como mogno (CARVALHO et al, 2016), Bauhinia forficata (GUARESCHI et al, 2015), leiteira (Tabernaemontana fuchsiaefolia) (MORAES et al, 2016), ipê roxo (LAMARCA & BARBEDO 2017), ipê (Handroanthus albus) (SHIBATA et al, 2012), aroeira (Myracrodruon urundeuva) (CALDEIRA & PEREZ, 2010), entre outras. Sendo assim, o objetivo desta pesquisa foi identificar procedimentos adequados para a condução do teste de envelhecimento acelerado para avaliação do vigor de sementes de Parapiptadenia rigida (Benth.)…”

Section: Introductionunclassified

ENVELHECIMENTO ACELERADO PARA AVALIACAO DO VIGOR DE SEMENTES DE Parapiptadenia rigida

Dotto¹,

Silva²

2017

Agrarian

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RESUMOA utilização de procedimentos eficientes para análise de sementes é fundamental para programas de controle de qualidade, auxiliando na escolha dos materiais de maior potencial para a produção de mudas, assim como para o monitoramento durante o armazenamento de sementes. O objetivo desta pesquisa foi identificar procedimentos adequados para o teste de envelhecimento acelerado para avaliação do vigor de sementes de Parapiptadenia rigida. Três lotes de sementes foram avaliados quanto ao teor de água e a germinação e vigor, por meio dos testes de condutividade elétrica, comprimento, massa fresca e seca de plântulas e com o teste de envelhecimento acelerado realizado com diferentes metodologias. Os resultados obtidos permitiram concluir que os testes de envelhecimento acelerado à 38°C por 24, 48 ou 72 horas e 41°C por 24 horas são eficientes na avaliação do vigor de sementes de Parapiptadenia rigida. PALAVRAS-CHAVE: angico, qualidade de sementes, tecnologia de sementes. ACCELERATED AGING TEST TO EVALUATE Parapiptadenia rígida SEED VIGORABSTRACT The use of efficient procedures for seed analysis is essential for quality control programs, helping to select the materials with the greatest potential for seedling production, as well as for monitoring during seed storage. The objective of this research was to identify appropriate procedures of accelerated aging test to evaluate Parapiptadenia rigida (Benth.) Brenan seed vigor. Parapiptadenia rigida seeds represented by 3 lots. Seed moisture content was measured and germination capacity were evaluated; seed vigor was evaluated by electrical conductivity, seedling length, fresh and dry seedling weight obtained after seedling length test and with different accelerated aging tests. Results showed that Parapiptadenia rigida seed vigor can be evaluated efficiently by accelerated aging tests, using combinations of 38 ° C for 24, 48 or 72 hours and 41 ° C for 24 hours.

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“…On the other hand, the AA test evaluates seed performance exposed to adverse conditions such as high temperatures and moisture which induce oxidative reactions in seed cell constituents, thus simulating what occurs in natural seed aging, but faster (MORAES et al, 2016). Knowing the characteristics of physiological and biochemical changes under AA conditions are useful to better understand the seed deterioration process.…”

Section: Introductionmentioning

confidence: 99%

Suitability of Electrical Conductivity Testing and Accelerated Aging for Evaluating the Vigor Ofcitharexylum Myrianthum Cham. Diaspores

Bazzanella

Mathias²,

Mittelstaedt³

et al. 2019

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The Atlantic Forest is one of the most threatened Brazilian biomes by anthropic action. Thus, the interest inrecovering degraded areas through the use of native tree species has increased, causing a high demand in the use of vigorous seeds. This research aimed at evaluating the suitabilityof the electrical conductivity and accelerated aging tests to evaluate the vigor of C. myrianthum diaspores. For the adequacy of the electrical conductivity test, a completely randomized experimental design (CRD) was used in a 2 x 3 x9 (number of diaspores x volume of water x imbibition time)at a constant temperature of 25 °C. The procedure for the accelerated aging test was conducted at 40 ºC for 0 (no aging), 24, 48 and 72 hours. ACRDwas also used for the accelerated aging test, totaling 4 treatments. The results were submitted to analysis of variance (ANOVA) and the means were compared by the Tukey test at 5% probability. It is concluded that the electrical conductivity test for C. myrianthum diaspores can be conducted with 25 diaspores soaked with 50 mL of deionized water for 96 hours, thus replacing the germination test with the advantage of being carried out in a shorter period of time. Moreover,the 24 and 48 hour accelerated aging period is adequate to discriminate the vigor of C. myrianthum diaspores, with GSI and MGT being the most sensitive variables.

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QUALIDADE FISIOLÓGICA DE SEMENTES DE <i>Tabernaemontana fuchsiaefolia</i> A. DC EM FUNÇÃO DO TESTE DE ENVELHECIMENTO ACELERADO¹

Cited by 16 publications

References 5 publications

PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS

PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS

ENVELHECIMENTO ACELERADO PARA AVALIACAO DO VIGOR DE SEMENTES DE Parapiptadenia rigida

Suitability of Electrical Conductivity Testing and Accelerated Aging for Evaluating the Vigor Ofcitharexylum Myrianthum Cham. Diaspores

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