Quadruplex amplification of polymorphic STR loci in a Korean population

Lee, Dong Hoon; Han, Jae Seok; Lee, Woo Ghil; Lee, Seong Whan; Rho, Hyune Mo

doi:10.1007/s004140050179

Cited by 17 publications

(10 citation statements)

References 11 publications

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“…Sequence data D18S535 (Lareu et al 1998 a) shows a conserved 4 bp and D10S2325 (Lee et al 1998) a conserved 5 bp array (Table 1). Two point mutations were found in the 3′ flanking region of D10S2325.…”

Section: Resultsmentioning

confidence: 99%

“…PCR primers and conditions: D18S535 primer 1: 5′ -TCA TGT GAC AAA AGC CAC AC, primer 2: 5′ -AGA CAG AAA TAT AGA TGA GAA TGC CA (Lareu et al 1998 a); D1S1656 primer 1: 5′ -GTG TTG CTC AAG GGT CAA CT (Lareu et al 1998 b); primer 2: 5′ -GAG AAA TAG AAT CAC TAG GGA ACC; D10S2325 primer 1: 5′ -CTC ACG AAA GAA GCC TTC TG; primer 2: 5′ -GAG CTG AGA GAT CAC GCA CT (Lee et al 1998); amplification protocol: 94°C -1 min, 61°C -1 min, 72°C -1 min; 30 cycles.…”

Section: Methodsunclassified

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D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics

Wiegand

Lareu²,

Schürenkamp

et al. 1999

Int J Leg Med

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Three short tandem repeat (STR) polymorphisms characterized by PCR product length < 175 bp were investigated. D18S535 and D1S1656 contained a 4 bp unit as basic repeat motif, D10S2325 a 5 bp unit. The heterozygosity rates were 0.76 (D18S535), 0.88 (D10S2325) and 0. 90 (D1S1656), leading to a combined discrimination power of 0.9999. In contrast to D10S2325 and D18S535, which showed a homogeneous repeat array without any variation in the repeat motifs, repeat length and sequence variation was found for D1S1656. Robust typing results could be observed for all three STRs using highly degraded DNA.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsunclassified

D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics

Wiegand

Lareu²,

Schürenkamp

et al. 1999

Int J Leg Med

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“…Analysis of mixed samples is possible 4. Specificity and sensitivity satisfy forensic requirements (Hochmeister et al 1991;Edwards et al 1992;Fregeau and Fourney 1993;Kimpton et al 1993;Urquhart et al 1995;Rolf et al 1997;Klintschar and Neuhuber 1998;Lee et al 1998;Wallin et al 1998). The co-amplification of several STR loci in one reaction saves time, material and cost with the additional benefits of conserving sample and reducing the risk of contamination (Wallin et al 1998).…”

Section: Introductionmentioning

confidence: 93%

A new pentaplex PCR system for forensic casework analysis

Lederer

Seidl²,

Graham³

et al. 2000

International Journal of Legal Medicine

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In 1998 the Federal Criminal Police Office of Germany (BKA) established a central genetic database of offenders and suspects to facilitate comparisons with biological samples from future criminal offences. The five obligatory short tandem repeat (STR) loci in this database (TH01, SE33, vWA, FGA and D21S11) were co-amplified in a new PCR pentaplex analysing system together with the sex-specific locus amelogenin. Due to overlapping fragment sizes, amplification products were fluorescent dye-labelled with different colours, separated by electrophoresis and detected directly using the ABI PRISM 310 Genetic Analyzer. Reproducible and reliable results were obtained from as low as 125 pg template DNA, indicating high specificity and sensitivity of the assay. Environmental studies and enzymatic digest with DNase I revealed an excellent stability of the pentaplex system with typeable results even in cases of partially degraded DNA. Complete and reproducible DNA typing was possible in blood-stain mixtures with the minor component as low as 10%. Mean stutter peak intensities were analysed for all loci and ranged from 2.7 +/- 0.8% (TH01) to 10.6 +/- 1.6% (vWA) of the main signal intensity. Allele frequencies were determined in a North Bavarian population sample (n = 121). The combination of five systems resulted in a mean exclusion chance of 99.86% and a power of discrimination of 99.999996%. No deviation from Hardy-Weinberg equilibrium could be found.

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“…After careful consideration, the following 15 markers were taken from the literature: D1S1656 [11][12][13], D7S1517 [14], D8S306 [15,16], D8S639 [17,18], D9S304 [19], D10S2325 [12,20], D11S488 [18,21], D12S391 [22][23][24][25], D14S608 [2,26], D16S3253 [26], D17S976 [27], D18S1270 [26], D19S253 [28], D20S161 [29], and D21S1437 [26]. The chromosomal locations of these 15 loci, as well as those of STR markers used in commercially available PCR multiplexes (e.g., Powerplex 16 system), were determined by searching the May 2004 human genome assembly using BLAT [30] (http://www.genome.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of an extended set of 15 candidate STR loci for paternity and kinship analysis in an Austrian population sample

et al. 2006

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We investigated 15 polymorphic short tandem repeat (STR) loci (D1S1656, D7S1517, D8S306, D8S639, D9S304, D10S2325, D11S488, D12S391, D14S608, D16S3253, D17S976, D18S1270, D19S253, D20S161, and D21S1437) which are not included in the standard sets of forensic loci. The markers were selected according to the complexity of the polymorphic region: Of the 15 investigated loci, 7 loci showed a simple repeat structure (D9S304, D10S2325, D14S608, D16S3253, D18S1270, D19S253, and D21S1437), 3 loci (D7S1517, D12S391, and D20S161) consisted of compound repeat units, and 5 loci (D1S1656, D8S306, D8S639, D11S488, and D17S976) showed a more complex polymorphic region partly including different repeat blocks and incomplete repeat units, which resulted in a relatively high proportion of intermediate alleles. A population study on a sample of 270 unrelated persons from Austria was carried out. We did not observe significant deviations from Hardy-Weinberg expectations. The combined probability of exclusion for the 15 loci was 0.99999998. In combination with the conventional set of STR markers included in commercially available kits (no linkage was observed between these 15 loci and the Powerplex 16 System loci), these markers are approved as highly discriminating forensic tools, also suitable for the analysis of difficult paternity and kinship constellations.

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Quadruplex amplification of polymorphic STR loci in a Korean population

Cited by 17 publications

References 11 publications

D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics

D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics

A new pentaplex PCR system for forensic casework analysis

Evaluation of an extended set of 15 candidate STR loci for paternity and kinship analysis in an Austrian population sample

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