Quadriwave lateral shearing interferometry as a quantification tool for microscopy. Application to dry mass determination of living cells, temperature mapping, and vibrational phase imaging

Monneret, Serge; Bon, Pierre; Baffou, Guillaume; Berto, Pascal; Savatier, Julien; Aknoun, Shérazade; Rigneault, Hervé

doi:10.1117/12.2021331

Cited by 3 publications

(2 citation statements)

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“…In cell microscopy, quantitative phase imaging (QPI) [1,2] holds a special position. QPI allows monitoring of adherent cell cultures continuously over several days [3][4][5][6]. QPI images of adherent cells are highly contrasted and can be further processed to quantify cellular features, related to morphology, density, and texture [7,8].…”

Section: Introductionmentioning

confidence: 99%

Quantitative phase imaging of adherent mammalian cells: a comparative study

Allier¹,

Hervé²,

Mandula³

et al. 2019

Biomed. Opt. Express

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The quantitative phase imaging methods have several advantages when it comes to monitoring cultures of adherent mammalian cells. Because of low photo-toxicity and no need for staining, we can follow cells in a minimally invasive way over a long period of time. The ability to measure the optical path difference in a quantitative manner allows the measurement of the cell dry mass, an important metric for studying the growth kinetics of mammalian cells. Here we present and compare cell measurements obtained with three different techniques: digital holographic microscopy, lens-free microscopy and quadriwave lateral sheering interferometry. We report a linear relationship between optical volume density values measured with these different techniques and estimate the precisions of this measurement for the different individual instruments.

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Section: Introductionmentioning

confidence: 99%

Quantitative phase imaging of adherent mammalian cells: a comparative study

Allier¹,

Hervé²,

Mandula³

et al. 2019

Biomed. Opt. Express

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“…A key advantage of these modifications is that they often lead to modular compatibility with commercial microscopes [7,8,[12][13][14][15][16][17][18], the results of which have been transformative for the biomedical application of QPI [2][3][4][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. Quantitative phase microscopy (QPM or QPI via microscopy) has been enabling for reasons such as the ability to perform multimodal investigations correlating QPI data with images from other microscopy modalities: for example, fluorescence [37].…”

Section: Introductionmentioning

confidence: 99%

Quantitative phase microscopy via optimized inversion of the phase optical transfer function

Jenkins

Gaylord

2015

Appl. Opt.

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Although the field of quantitative phase imaging (QPI) has wide-ranging biomedical applicability, many QPI methods are not well-suited for such applications due to their reliance on coherent illumination and specialized hardware. By contrast, methods utilizing partially coherent illumination have the potential to promote the widespread adoption of QPI due to their compatibility with microscopy, which is ubiquitous in the biomedical community. Described herein is a new defocus-based reconstruction method that utilizes a small number of efficiently sampled micrographs to optimally invert the partially coherent phase optical transfer function under assumptions of weak absorption and slowly varying phase. Simulation results are provided that compare the performance of this method with similar algorithms and demonstrate compatibility with large phase objects. The accuracy of the method is validated experimentally using a microlens array as a test phase object. Lastly, time-lapse images of live adherent cells are obtained with an off-the-shelf microscope, thus demonstrating the new method's potential for extending QPI capability widely in the biomedical community.

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