QTLs Related to Rice Callus Regeneration Ability: Localization and Effect Verification of qPRR3

Abstract

Mature and efficient tissue culture systems are already available for most japonica rice varieties (Oryza sativa ssp. geng). However, it remains challenging to regenerate the majority of indica rice varieties (Oryza sativa ssp. xian). In this study, quantitative trait loci (QTLs) associated with rice callus regeneration ability were identified based on the plant regeneration rate (PRR) and total green plant rate (TGPR) of the 93-11 × Nip recombinant inbred line population. Significant positive correlations were found between PRR and TGPR. A total of three QTLs (one for PRR and two for TGPR) were identified. qPRR3 (located on chromosome 3) was detected for both traits, which could explain 13.40% and 17.07% of the phenotypic variations of PRR and TGPR, respectively. Subsequently, the effect of qPRR3 on callus regeneration ability was validated by cryptographically tagged near-isogenic lines (NILs), and the QTL was narrowed to an interval of approximately 160 kb. The anatomical structure observation of the regenerated callus of the NILs revealed that qPRR3 can improve the callus regeneration ability by promoting the regeneration of shoots.

Unveiling the Genetic Basis Underlying Rice Anther Culturability via Segregation Distortion Analysis in Doubled Haploid Population

“…Previous studies have consistently shown that cultivated rice displays a greater capacity for anther culture when compared to wild rice [11]. Furthermore, within cultivated rice varieties, japonica rice has been found to exhibit significantly higher efficiency in anther culture compared to indica rice [12][13][14][15].…”

CRISPR-Cas system: revolutionizing plant breeding and gene therapy

“…The transformation frequency has a great relationship with the plant genotypes. For example, the comparison of six rice varieties (Oryza sativa L.), one of the species with the highest transformation rate, showed that different genotypes have different transformation efficiency to Agrobacterium tumefaciens [23]. Moreover, even in plants with relatively high transformation frequency (such as tobacco, potato, Arabidopsis, etc.…”

Organelle Ca2+/CAM1‐SELTP confers somatic cell embryogenic competence acquisition and transformation in plant regeneration

“…In recent years, a series of genes that play roles in somatic embryogenesis (SE) have been cloned and studied. Previous studies reported that the overexpression (OE) of several classic genes, including WUSCHEL ( WUS ; Lopes et al ., 2021; Wang et al ., 2022; Lu et al ., 2023; Ogura et al ., 2023), BABY BOOM ( BBM ; Li et al ., 2022; N. Wang et al ., 2023), WRKY (Zhang et al ., 2024) and LEAFY COTYLEDON1 ( LEC1 ; Wang et al ., 2009), is able to cause vegetative‐to‐embryonic transition or plant regeneration (J. Wu et al ., 2022; Yu et al ., 2023). In addition, recent advances have updated SE‐related genes, including GhRCD1 (Yuan et al ., 2023), GROWTH‐REGULATING FACTORs (GRFs; Feng et al ., 2021; Luo & Palmgren, 2021), SCARECROW‐LIKE genes CsSCL2 and CsSCL3 ( CsSCL2/3 ; Feng et al ., 2023).…”