QTLs for a component of partial resistance to cucumber mosaic virus in pepper: restriction of virus installation in host-cells

Caranta, Carole; Palloix, Alain; Lefebvre, Véronique; Daubèze, A.M.

doi:10.1007/s001220050433

Cited by 97 publications

(57 citation statements)

References 28 publications

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“…We named these resistance genes 'cmr3E' and 'cmr3L' respectively. Studies for the resistance to CMV in C. annuum have been reported many research groups, most of them were incomplete dominant and quantitatively inherited (Caranta et al, 1997(Caranta et al, , 2002Grube et al, 2000), except for Cmr1 (Kang et al, 2010). Cmr1 on the LG2 is the only dominant resistance gene known in pepper, but resistance is broken down by CMV P1 strain, requiring more robust CMV resistant germplasm (Kang et al, 2010).…”

Section: Inheritance Of Resistance To CMVmentioning

confidence: 99%

“…In some cucurbits, resistance to CMV was controlled by one recessive gene (Kang et al, 2005), tomato and Arabidopsis have single dominant gene for resistance (Stamova and Chetelat, 2000;Takahashi et al, 2001Takahashi et al, , 2002. Several poly-genes for resistance were reported in Capsicum species including 'Perennial' (Caranta et al, 1997;Chaim et al, 2001;Grube et al, 2000;Lapidot et al, 1997), 'Vania' (Caranta et al, 2002), C. baccatum 'PI439381-1-3' (Suzuki et al, 2003) and C. frutescens 'BG2814-6' (Grube et al, 2000). In Arabidopsis and tomato, single dominant gene RCY and Cmr were suggested for resistance (Stamova and Chetelat, 2000;Takahashi et al, 2001Takahashi et al, , 2002.…”

Section: Introductionmentioning

confidence: 99%

“…In Arabidopsis and tomato, single dominant gene RCY and Cmr were suggested for resistance (Stamova and Chetelat, 2000;Takahashi et al, 2001Takahashi et al, , 2002. Up to date, three different steps of inhibition of the virus cycles were reported in distinct resistant Capsicum accessions: restriction of host-cell infection (Caranta et al, 1997), suppression of virus multiplication in the whole plants (Nono-Wondim et al, 1993) and restraint of longdistance movement (Dufour et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Developmental Changes of Recessive Genes-mediated Cucumber mosaic virus (CMV) Resistance in Peppers (Capsicum annuum L.)

Min¹,

Ryu²,

Ahn³

2014

HST

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Cucumber mosaic virus (CMV) is one of the most important viral diseases in pepper (Capsicum annuum L.), and several genes for resistance were reported in Capsicum spp. In Korea, a single dominant gene that is resistant to CMV Fny and CMV P0 has been used for breeding. Recently, a new strain (CMV P1) was reported that could infect cultivars resistant to both CMV Fny and CMV P0. Therefore, breeding of more robust CMV-resistant cultivars is required. In this study, we surveyed the inheritance of CMV P1 resistance and analyzed the location of the resistance loci. After CMV P1 inoculation of various germplasms and breeding lines, one accession (ICPN18-8) showed no visual symptoms at 15 dpi (days post inoculation) but was susceptible after 45 dpi, and one resistant line (I7339) showed resistance until at 45 dpi. The latter line was used for tests of resistance inheritance. A total of 189 F2 plants were examined, with 42 individuals showing resistance at 15 dpi and a phenotype segregation ratio close to 1:3 (resistant:susceptible plants). In a lateral ELISA test at 45 dpi, 11 plants showed resistance, and the segregation ratio was changed to 1:15. These results indicate that resistance in C. annuum 'I7339' is controlled by two different recessive genes; we named these resistance genes 'cmr3E' and 'cmr3L,' respectively. To locate these two resistant loci in the pepper linkage map, various RAPD, SSR, and STS markers were screened; only nine markers were grouped into one linkage group (LG). Only one RAPD primer (OPAT16) was distantly linked with cmr3E (22.3 cM) and cmr3L (20.7 cM). To develop more accurate markers for marker-assisted breeding, enriching for molecular markers spanning two loci will be required. Additional key words: linkage group, RAPD

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Section: Inheritance Of Resistance To CMVmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Developmental Changes of Recessive Genes-mediated Cucumber mosaic virus (CMV) Resistance in Peppers (Capsicum annuum L.)

Min¹,

Ryu²,

Ahn³

2014

HST

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“…많은 기주범위와 바이러스병 발생빈도가 높은 반면 이에 대한 뚜렷한 방제법 이 확립되지 못한 실정이다. CMV 저항성 고추로 인도에서 발견된 Perennial이 저항성 재료로 보고되었고, 이를 이용한 저항성 육종 (Lapidot et al, 1997), QTL mapping (Caranta et al, 1997(Caranta et al, , 2002Chaim et al, 2001), 새로운 저항성 유전자 의 검색 (Grube et al, 2000;Suzuki et al, 2003) 등의 연구 가 진행되고 있다. 국내에서는 고추에 발생하는 바이러스병 의 분류 동정 Kang et al, 1973;Kim and Choi, 2001;Kim et al, 1990aKim et al, , 1990bKim et al, , 2002Lee et al, 2002;Im et al, 1991), 방제 (Choi et al, 1995;Lim et al, 1997;Sung et al, 2002)에 관한 연구보고는 있으나 고추의 바이러스병 저항성과 관련해서는 Kang and Choi(1976) (Cai et al, 2003;Chen et al, 2003;Lee et al, 2009).…”

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CMV-P1 Resistance Evaluation Using Enzyme-linked Immunosorbent Assay of Pepper Genetic Sources (Capsicum spp.)

Shin¹,

Xu²,

Kim³

et al. 2013

Korean Journal of Horticultural Science and Technology

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Abstract.Recently, Cucumber mosaic virus (CMV)-P1 infection in pepper cultivation is very serious problem, which causes low marketability and yield. In this research, 56 domestic pepper cultivars including 20 PR (Phytophthora resistance) cultivars, 31 foreign pepper lines collected from USA and 112 genetic pepper resources form RDA Genebank were used for CMV-P1 resistance evaluation. Resistance evaluation was performed at 24 days and 51 days after artificial inoculation of peppers by enzyme-linked immunosorbent assay (ELISA). Among the 36 domestic cultivars 'Muhanjilju' were resistant to CMV-P1. All 20 PR cultivars and 29 foreign peppers except 'NuMex Twilight' and 'Chainese Giant' were susceptible. Among 112 pepper lines from RDA Genebank, nine pepper lines were resistant, and 17 pepper lines were moderately resistant, and 86 pepper lines were susceptible. Almost all domestic peppers on the market were highly susceptible to CMV-P1, whereas 17.2% of foreign pepper cultivars and genetic pepper lines from RDA Genebank were resistant or moderately resistant. Resistant pepper lines selected in this study can be used as genetic sources for breeding CMV-P1 resistant pepper.

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“…Sometimes systemic hypersensitivity occurs (Murphy and Kyle, 1995;Tatineni and French, 2014) Resistance to virus movement between cells Virus gains entry into plant virus is sequestrated to islands of cells without induction of necrosis Plant defence mechanism confines the virus to inoculated cells (Lecoq et al, 2004) Complete resistance (immunity) There's contact between plant and virus but virus does not either gain entry or is not allowed to replicate after entry Virus may lack some necessary factors for pathogenesis or the plant's defence is strong to combat virus replication (Anjanappa et al, 2016;Kohm et al, 1993;Legnani et al, 1995) Resistance due to escape of infection mature plant escaping infection at adult stage but not earlier (same level of inoculum is used) Virus does not overcome the initial passive defence barriers of the plant (rigid cell walls) (Caranta et al, 1997) Resistance to inoculation by vectors There is no contact between plant and virus Vectors are not able to transmit the virus for some reason (Jones, 1998;Lecoq et al, 1979) While there are different types of small RNAs in plants, interestingly, they are generated by a related set of multiple genes following different pathways. There are four major sets of protein families which include Dicer-like, dsRNA binding, RNA-dependent RNA polymerase (RDRPs) and…”

Section: Rna Silencing As An Antiviral Defence Mechanism By Plantsmentioning

confidence: 99%

Assessment of Banana Streak MY virus-based infectious clone vectors in Musa ssp.

Onsarigo¹

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Banana streak disease, caused by a complex of different banana streak viruses (BSVs), occurs in all banana-producing countries. BSVs are genetically very diverse and can exist both as episomal forms or integrated into the host genome and both can be infectious to banana.Several field studies have assessed the infectivity of one or more BSV species on different banana genotypes with significant differences reported in virus incidence and symptom expression between and within genotypes. These studies used a severity index, based on symptom expression, to determine the resistance/susceptibility levels of the genotypes.Because of the great genetic variability amongst BSV species, symptoms of banana streak disease may vary widely and may be influenced by the cultivar and/or environmental conditions. Also this system of screening does not reflect the level of viral DNA accumulation In the current study, symptom variability was considerable with different Musa genotypes expressing distinct symptoms. Quantification of the levels of virus DNA showed that virus accumulation differs between genotypes, but also within plants of the same accession, and that severity scores do not correlate well with the viral load as measured using qPCR.Modified vectors with either ORF1 or ORF2 deleted were not infectious and the addition of a GFP ORF into two sites in the genome did not result in expression of this heterologous sequence from the constructs used in this study. Interestingly, two constructs with small inserts, initially designed for the purpose of gene silencing, were found to retain infectivity and maintain a heterologous sequence up to 110 nt. These studies confirm that BSMYV is infective in a broad range of Musa genotypes and cultivars but that further work is required to develop suitable vectors for either gene expression or gene silencing studies in banana

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QTLs for a component of partial resistance to cucumber mosaic virus in pepper: restriction of virus installation in host-cells

Cited by 97 publications

References 28 publications

Developmental Changes of Recessive Genes-mediated Cucumber mosaic virus (CMV) Resistance in Peppers (Capsicum annuum L.)

Developmental Changes of Recessive Genes-mediated Cucumber mosaic virus (CMV) Resistance in Peppers (Capsicum annuum L.)

CMV-P1 Resistance Evaluation Using Enzyme-linked Immunosorbent Assay of Pepper Genetic Sources (Capsicum spp.)

Assessment of Banana Streak MY virus-based infectious clone vectors in Musa ssp.

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