QTL underlying plant and first branch height in cassava (Manihot esculenta Crantz)

Boonchanawiwat, A.; Sraphet, Supajit; Boonseng, Opas; Lightfoot, David A.; Triwitayakorn, Kanokporn

doi:10.1016/j.fcr.2010.12.022

Cited by 21 publications

(20 citation statements)

References 26 publications

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“…), plant height and first branch height (Boonchanawiwat et al . ). However, QTL controlling starch pasting properties in cassava have not yet been identified, partly due to concerns about the low variation observed in starch quality.…”

Section: Introductionmentioning

confidence: 97%

Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (Manihot esculenta Crantz)

et al. 2013

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Starch pasting viscosity is an important quality trait in cassava (Manihot esculenta Crantz) cultivars. The aim here was to identify loci and candidate genes associated with the starch pasting viscosity. Quantitative trait loci (QTL) mapping for seven pasting viscosity parameters was carried out using 100 lines of an F1 mapping population from a cross between two cassava cultivars Huay Bong 60 and Hanatee. Starch samples were obtained from roots of cassava grown in 2008 and 2009 at Rayong, and in 2009 at Lop Buri province, Thailand. The traits showed continuous distribution among the F1 progeny with transgressive variation. Fifteen QTL were identified from mean trait data, with Logarithm of Odds (LOD) values from 2.77-13.01 and phenotype variations explained (PVE) from10.0-48.4%. In addition, 48 QTL were identified in separate environments. The LOD values ranged from 2.55-8.68 and explained 6.6-43.7% of phenotype variation. The loci were located on 19 linkage groups. The most important QTL for pasting temperature (PT) (qPT.1LG1) from mean trait values showed largest effect with highest LOD value (13.01) and PVE (48.4%). The QTL co-localised with PT and pasting time (PTi) loci that were identified in separate environments. Candidate genes were identified within the QTL peak regions. However, the major genes of interest, encoding the family of glycosyl or glucosyl transferases and hydrolases, were located at the periphery of QTL peaks. The loci identified could be effectively applied in breeding programmes to improve cassava starch quality. Alleles of candidate genes should be further studied in order to better understand their effects on starch quality traits.

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Section: Introductionmentioning

confidence: 97%

Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (Manihot esculenta Crantz)

et al. 2013

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“…Newly developed SSR primers were designed based on the sequences of the 200 kilobase pairs (kbp) covering regions of the PR genes from the cassava genome database using the primer design tool WebSat (http://wsmartins.net/websat/). Genomic DNA was amplified by PCR and products analysed by electrophoresis on 50 g/l polyacrylamide gels as described previously (Boonchanawiwat et al 2011). Amplification products were visualized by the silver staining method as described by Benbouza et al (2006).…”

Section: Genetic Linkage Map Constructionmentioning

confidence: 99%

“…Several genetic maps of cassava have been published based on different types of molecular markers such as restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), isoenzymes, amplified fragment length polymorphism (AFLP), simple sequence repeat (SSR) and expressed sequence tag-SSR (EST-SSR) markers (Mba et al 2001;Okogbenin et al 2006;Chen et al 2010;Kunkeaw et al 2010bKunkeaw et al , 2011Sraphet et al 2011). The maps have been applied to the identification of quantitative trait loci (QTL) including those controlling early root growth (Okogbenin & Fregene 2002), root productivity and plant architecture (Okogbenin & Fregene 2003), root dry matter and cyanogen content (Balyejusa et al 2007), components of early root yield (Okogbenin et al 2008), plant and first branch height (Boonchanawiwat et al 2011), fresh weight root yield, root dry matter content, root starch content (Chen et al 2012), starch pasting viscosity (Thanyasiriwat et al 2014) and cyanogen content (Whankaew et al 2011). However, there have been no studies identifying QTL linked to CAD, although molecular markers (derived by the RAPD technique) linked to CAD resistance in cassava have been discovered (Akinbo et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Mapping of quantitative trait loci underlying resistance to cassava anthracnose disease

et al. 2015

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SUMMARYCassava (Manihot esculenta Crantz) is an economically important root crop in Thailand, which is ranked the world's top cassava exporting country. Production of cassava can be hampered by several pathogens and pests. Cassava anthracnose disease (CAD) is an important disease caused by the fungus Colletotrichum gloeosporioides f. sp. manihotis. The pathogen causes severe stem damage resulting in yield reductions and lack of stem cuttings available for planting. Molecular studies of cassava response to CAD will provide useful information for cassava breeders to develop new varieties with resistance to the disease. The current study aimed to identify quantitative trait loci (QTL) and DNA markers associated with resistance to CAD. A total of 200 lines of two F1 mapping populations were generated by reciprocal crosses between the varieties Huabong60 and Hanatee. The F1 samples were genotyped based on simple sequence repeat (SSR) and expressed sequence tag-SSR markers and a genetic linkage map was constructed using the JoinMap®/version3·0 program. The results showed that the map consisted of 512 marker loci distributed on 24 linkage groups with a map length of 1771·9 centimorgan (cM) and a mean interval between markers of 5·7 cM. The genetic linkage map was integrated with phenotypic data for the response to CAD infection generated by a detached leaf assay test. A total of three QTL underlying the trait were identified on three linkage groups using the MapQTL®/version4·0 program. Those DNA markers linked to the QTL that showed high statistically significant values with the CAD resistance trait were identified for gene annotation analysis and 23 candidate resistance genes to CAD infection were identified.

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“…However, some bottlenecks such as a low plant material multiplication rate still remain as a challenge for cassava growers and producers (Ceballos et al 2015). The conventional method of cassava planting via asexual propagation, coupled with the long cycle of the crop, has hampered the development and implementation of crop breeding programs (Bredeson et al 2016); this is because it takes about five to six years between the parents' hybridization and the initial cycles of evaluation and selection to complete one breeding cycle (Boonchanawiwat et al 2011, Okogbenin et al 2012. For growers, the biggest challenge is to produce enough propagating material to replace obsolete varieties with new improved varieties.…”

Section: Introductionmentioning

confidence: 99%

Productive potential of cassava plants (Manihot esculenta Crantz) propagated by leaf buds

Neves

Diniz

Oliveira

2018

An. Acad. Bras. Ciênc.

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New techniques of rapid multiplication of cassava (Manihot esculenta Crantz) have been developed, requiring technical support for large-scale use. This work main to evaluate the agronomic performance of plantlets obtained by leaf buds technique against stem cuttings in the field conditions. The work was conducted using the randomized block design in a factorial scheme with 3 varieties (BRS Kiriris, 98150-06, 9624-09) × 4 origins of the plantlets (conventional - stem cuttings of 20 cm length, leaf buds of the upper, middle and inferior stem part) × 2 agrochemicals (control and treated). There was a remarkable decrease in some agronomic traits that ranged from 23% (number of branches) to 62% (shoot weight) when using leaf buds plantlets. The treatment of plantlets with agrochemicals promoted significant increases in all traits, ranging from 26% (number of roots per plant) to 46% (shoot weight). The plantlets originating from leaf buds of the upper and middle parts were able to generate stem-like plants similar to stem-derived ones. Despite its lower agronomic performance under field conditions, multiplication by leaf buds may generate five times the number of propagules in comparison with the conventional multiplication, and therefore it could be a viable alternative for rapid cassava multiplication.

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QTL underlying plant and first branch height in cassava (Manihot esculenta Crantz)

Cited by 21 publications

References 26 publications

Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (Manihot esculenta Crantz)

Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (Manihot esculenta Crantz)

Mapping of quantitative trait loci underlying resistance to cassava anthracnose disease

Productive potential of cassava plants (Manihot esculenta Crantz) propagated by leaf buds

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