QTL mapping of seed protein and oil traits in two recombinant inbred line soybean populations

Gillenwater, Jay H.; McNeece, Brant T.; Taliercio, Earl; Mian, M. A. Rouf

doi:10.1080/15427528.2021.1985028

Cited by 1 publication

(1 citation statement)

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“…Previous studies have identified minor effect seed protein and oil QTLs in other regions of the chromosome 2 as in the report by Gillenwater et al [55] who identified four minor effect QTL: one for protein, one for oil and two for both seed protein and oil content each explaining less than 10% of the phenotypic variation. Kabelka et al [56] identified a QTL on LG D1b (chromosome 2) that explained 14% of the phenotypic variation for protein content.…”

Section: Discussionmentioning

confidence: 92%

Identification of quantitative trait loci controlling soybean seed protein and oil content

et al. 2023

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Soybean is a major source of seed protein and oil globally with an average composition of 40% protein and 20% oil in the seed. The goal of this study was to identify quantitative trait loci (QTL) conferring seed protein and oil content utilizing a population constructed by crossing an above average protein content line, PI 399084 to another line that had a low protein content value, PI 507429, both from the USDA soybean germplasm collection. The recombinant inbred line (RIL) population, PI 507429 x PI 399084, was evaluated in two replications over four years (2018–2021); the seeds were analyzed for seed protein and oil content using near-infrared reflectance spectroscopy. The recombinant inbred lines and the two parents were re-sequenced using genotyping by sequencing. A total of 12,761 molecular markers, which came from genotyping by sequencing, the SoySNP6k BeadChip and selected simple sequence repeat (SSR) markers from known protein QTL chromosomal regions were used for mapping. One QTL was identified on chromosome 2 explaining up to 56.8% of the variation for seed protein content and up to 43% for seed oil content. Another QTL identified on chromosome 15 explained up to 27.2% of the variation for seed protein and up to 41% of the variation for seed oil content. The protein and oil QTLs of this study and their associated molecular markers will be useful in breeding to improve nutritional quality in soybean.

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Section: Discussionmentioning

confidence: 92%