QTL mapping of root traits in wheat under different phosphorus levels using hydroponic culture

Yang, Mengjiao; Wang, Cairong; Hassan, Muhammad Adeel; Li, Faji; Xia, Xianchun; Shi, Suhua; Xiao, Yonggui; He, Zhonghu

doi:10.1186/s12864-021-07425-4

Cited by 26 publications

(21 citation statements)

References 47 publications

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“…MRL is an indication of root development potential for wheat yield improvement ( Li et al, 2021a ). Similar to previous studies ( Li et al, 2019 ; Yang et al, 2021 ), MRL was strongly correlated with other RSA-related traits except RD and RN in this study ( Table 2 ). By increasing the RA, RT, RV, and TRL, the contact area between roots and soil can be increased leading to the improvement of the efficiency of nutrient absorption for plants ( Ryser, 2006 ).…”

Section: Discussionsupporting

confidence: 91%

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Identification and validation of a locus for wheat maximum root length independent of parental reproductive environment

Chen

Zhao²,

Yang³

et al. 2022

Front. Plant Sci.

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Maximum root length (MRL) plays an important role in the uptake of nutrients and resisting abiotic stresses. Understanding the genetic mechanism of root development is of great significance for genetic improvement of wheat. Previous studies have confirmed that parental reproductive environment (PRE) has a significant impact on growth and development of the next generation in the whole life cycle of a given plant. In this study, a recombinant inbred line population genotyped using the Wheat55K SNP array, was used to map quantitative trait loci (QTL) for wheat seedling MRL based on the harvested seeds from five different PREs. A total of 5 QTL located on chromosomes 3D and 7A were identified. Among them, QMrl.sicau-2SY-3D.2 located in a 4.0 cM interval on chromosome 3D was likely independent of PREs. QMrl.sicau-2SY-7A.2 was detected in two tests and probably influenced by PREs. The effect of QMrl.sicau-2SY-3D.2 was further validated using the tightly linked kompetitive allele specific PCR (KASP) marker, KASP-AX-111589572, in populations with different genetic backgrounds. Lines with a combination of positive alleles from QMrl.sicau-2SY-3D.2 and QMrl.sicau-2SY-7A.2 have significantly longer MRL. Furthermore, four genes (TraesCS3D03G0612000, TraesCS3D03G0608400, TraesCS3D03G0613600, and TraesCS3D03G0602400) mainly expressed in wheat root were predicted to be associated with root growth. Taken together, this study reports on a major QTL independent of PREs and lays a foundation for understanding the regulation mechanism of wheat MRL at the seedling stage.

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Section: Discussionsupporting

confidence: 91%

“…Besides, QRL.caas-7AL was mapped between AX-109966788 and AX-94819074 (731.90–742.24 Mb) on chromosome arm 7AL ( Yang et al, 2021 ), suggested that it might be allelic to QMrl.sicau-2SY-7A.2 (652.28–669.74 Mbp).…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of a locus for wheat maximum root length independent of parental reproductive environment

Chen

Zhao²,

Yang³

et al. 2022

Front. Plant Sci.

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“…Similarly, previous research has been reported ( Su et al., 2009 ; Vijayalakshmi et al., 2010 ; Yang et al., 2016b ). The majority of chlorophyll content QTLs detected under CK, MP, and LP conditions are different, indicating that the chlorophyll regulation mechanism under low phosphorus conditions may differ from that under normal phosphorus conditions, and similar phenomena have been observed in previous research ( Yang et al., 2018 ; Yuan et al., 2020 ; Yang et al., 2021 ). Under multiple phosphorus conditions, six major QTLs Qchl.saw-2B.1 , Qchl.saw-3B.1 , Qchl.saw-4D.1 , Qchl.saw-4D.2 , Qchl.saw-5A.9 and Qchl.saw-6A.4 were detected ( Table 4 ).…”

Section: Discussionsupporting

confidence: 79%

Identification and validation of quantitative trait loci for chlorophyll content of flag leaf in wheat under different phosphorus treatments

Yang

Chen²,

Dang³

et al. 2022

Front. Plant Sci.

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In wheat, the leaf chlorophyll content in flag leaves is closely related to the degree of phosphorus stress. Identifying major genes/loci associated with chlorophyll content in flag leaves under different phosphorus conditions is critical for breeding wheat varieties resistant to low phosphorus (P). Under normal, medium, and low phosphorus conditions, the chlorophyll content of flag leaves was investigated by a double haploid (DH) population derived from a cross between two popular wheat varieties Jinmai 47 and Jinmai 84, at different grain filling stages. Chlorophyll content of the DH population and parents decreased gradually during the S1 to the S3 stages and rapidly at the S4 stage. At the S4 stage, the chlorophyll content of the DH population under low phosphorus conditions was significantly lower than under normal phosphate conditions. Using a wheat 15K single-nucleotide polymorphism (SNP) panel, a total of 157 QTLs were found to be associated with chlorophyll content in flag leaf and were identified under three phosphorus conditions. The phenotypic variation explained (PVE) ranged from 3.07 to 31.66%. Under three different phosphorus conditions, 36, 30, and 48 QTLs for chlorophyll content were identified, respectively. Six major QTLs Qchl.saw-2B.1, Qchl.saw-3B.1, Qchl.saw-4D.1, Qchl.saw-4D.2, Qchl.saw-5A.9 and Qchl.saw-6A.4 could be detected under multiple phosphorus conditions in which Qchl.saw-4D.1, Qchl.saw-4D.2, and Qchl.saw-6A.4 were revealed to be novel major QTLs. Moreover, the closely linked SNP markers of Qchl.saw-4D.1 and Qchl.saw-4D.2 were validated as KASP markers in a DH population sharing the common parent Jinmai 84, showed extreme significance (P <0.01) in more than three environments under different phosphorus conditions, which has the potential to be utilized in molecular marker-assisted breeding for low phosphorus tolerance in wheat.

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“…To identify the localization of T. aestivum PSTOL1 orthologs with QTLs for morpho-physiological and biochemical traits under phosphorus starvation, QTLs and linked molecular markers were retrieved from previous publications [ 38 , 39 , 40 , 41 , 42 , 43 ], and co-localization was shown as described previously in [ 44 ]. To obtain the physical position, each marker sequence or marker name was BLAST against the grain gene database [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

Role of Wheat Phosphorus Starvation Tolerance 1 Genes in Phosphorus Acquisition and Root Architecture

Abbas¹,

Naeem²,

Rubab

et al. 2022

Genes

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The wheat plant requires elevated phosphorus levels for its normal growth and yield, but continuously depleting non-renewable phosphorus reserves in the soil is one of the biggest challenges in agricultural production worldwide. The Phosphorus Starvation Tolerance 1 (PSTOL1) gene has been reported to play a key role in efficient P uptake, deeper rooting, and high yield in rice. However, the function of the PSTOL1 gene in wheat is still unclear. In this study, a total of 22 PSTOL1 orthologs were identified in the wheat genome, and found that wheat PSTOL1 orthologs are unevenly distributed on chromosomes, and these genes were under strong purifying selection. Under different phosphorus regimes, wheat PSTOL1 genes showed differential expression patterns in different tissues. These results strengthen the classification of Pakistan-13 as a P-efficient cultivar and Shafaq-06 as a P-inefficient cultivar. Phenotypic characterization demonstrated that Pakistan-13 wheat cultivar has significantly increased P uptake, root length, root volume, and root surface area compared to Shafaq-06. Some wheat PSTOL1 orthologs are co-localized with phosphorus starvation’s related quantitative trait loci (QTLs), suggesting their potential role in phosphorus use efficiency. Altogether, these results highlight the role of the wheat PSTOL1 genes in wheat P uptake, root architecture, and efficient plant growth. This comprehensive study will be helpful for devising sustainable strategies for wheat crop production and adaptation to phosphorus insufficiency.

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QTL mapping of root traits in wheat under different phosphorus levels using hydroponic culture

Cited by 26 publications

References 47 publications

Identification and validation of a locus for wheat maximum root length independent of parental reproductive environment

Identification and validation of a locus for wheat maximum root length independent of parental reproductive environment

Identification and validation of quantitative trait loci for chlorophyll content of flag leaf in wheat under different phosphorus treatments

Role of Wheat Phosphorus Starvation Tolerance 1 Genes in Phosphorus Acquisition and Root Architecture

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