QTL Mapping of Resistance to Thrips palmi Karny in Common Bean

Abstract

Host plant resistance in common bean (Phaseolus vulgaris L.) is a promising component in an integrated cropping system for managing thrips (Thrips palmi Karny) infestation. This study was conducted to identify quantitative trait loci (QTLs) for resistance to thrips in common bean, using F5:7 recombinant inbred lines (RILs) as a mapping population. The RILs, derived by single seed descent (SSD) of the cross of two Mesoamerican bean lines, BAT 881 and G 21212, were found to show transgressive segregation for thrips resistance in the field. Correlations between damage and reproductive adaptation (RA) scores were significant within and between seasons. The QTLs for both traits were located based on single interval mapping (IM) and joint interval mapping (JIM) analysis using a genetic map constructed with microsatellite and random amplified polymorphic DNA (RAPD) markers. Eight of eleven resulting linkage groups (LGs) were shown to be homologous to chromosomes of the integrated linkage map of common bean. A major QTL for thrips resistance located on LG b06 explained up to 26.8% of variance for resistance in a single season and was named Tpr6.1. The JIM across several seasons revealed various QTLs on LGs b02, b03, b06, and b08, some of which were located at regions of genes encoding for disease resistance. The identification and mapping of thrips‐resistance genes is expected to facilitate the development of resistant bean cultivars by using molecular marker‐assisted selection.

Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding

“…Symbols in subscript represent the source population of the QTL. AG: A55/G 122 , BA: Belneb-RR-1/A55 Fourie et al, 2004;Jung et al, 2003), BE: Berna/EMP 419 (Murray et al, 2004a,b), BG: BAT 881/G 21212 (Frei et al, 2005), BJ: BAT 93/Jalo EEP558 (Freyre et al, 1998;Gepts, 1999;Geffroy et al, 2000), BH: BAC 6/HT 7719 (Jung et al, 1996), BN: Bunsi/Newport (Kolkman & Kelly, 2003), BR: Bunsi/Raven (Ender & Kelly, 2005), B60: Benton/NY6020-4 (Miklas et al, 2003b), DG: DOR 364/G 19833 (López et al, 2003), DX: DOR 364/XAN 176 (Miklas et al, 2000c), H95: HR67/OAC 95 (Yu et al, 2004), MF: Montcalm/FR266 (Schneider et al, 2001), PX: PC50/XAN 159 (Jung et al, 1997(Jung et al, , 1998Park et al, 2001), RN: Red Hawk/Negro San Luis (Román-Avilés & , S95: Seaforth/OAC 95 (Tar'an et al, 2001), and XC: XR-235-1-1/Calima (Yu et al, 1998). Gene and QTL locations are approximate because most were not directly mapped in the BAT 93/Jalo EEP558 population.…”

RAPD Markers Linked to Late Blight Resistance in Tomato

“…Spaner et al (1998) mapped the loci affecting resistance to powdery mildew, leaf rust, stem rust, scald and net blotch in barley using field-scored data of disease severity under natural infestation. Natural infestation was also used by Frei et al (2005) to identify QTLs resistance to thrips in common bean.…”

The control of eriophyoid mites: state of the art and future challenges

“…The utilisation of these native insect and mite resistance genes can be an important factor in their management (Frei et al 2005). This can be achieved by classical breeding as well as by genetic engineering (Zhu et al 2006).…”