QTL mapping of physiological traits associated with salt tolerance in Medicago truncatula Recombinant Inbred Lines

Arraouadi, Soumaya; Badri, Mounawer; Abdelly, Chédly; Huguet, Thierry; Aouani, Mohamed Elarbi

doi:10.1016/j.ygeno.2011.11.005

Cited by 46 publications

(36 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A significant effect of genotype was observed for all traits. It is usually observed that shoot biomass traits have larger broad-sense heritability that root biomass traits (Arraouadi et al , 2012; Bouteillé et al , 2012), and the results obtained with the pouch-and-wick system confirm these observations (Table 3). Broad-sense heritability of root length typically ranges from 0.15 to 0.80 (Gahoonia and Nielsen, 2004; Bouteillé et al , 2012).…”

Section: Discussionsupporting

confidence: 61%

A scanner system for high-resolution quantification of variation in root growth dynamics of Brassica rapa genotypes

Adu

Chatot

Wiesel

et al. 2014

Journal of Experimental Botany

106

View full text Add to dashboard Cite

The potential exists to breed for root system architectures that optimize resource acquisition. However, this requires the ability to screen root system development quantitatively, with high resolution, in as natural an environment as possible, with high throughput. This paper describes the construction of a low-cost, high-resolution root phenotyping platform, requiring no sophisticated equipment and adaptable to most laboratory and glasshouse environments, and its application to quantify environmental and temporal variation in root traits between genotypes of Brassica rapa L. Plants were supplied with a complete nutrient solution through the wick of a germination paper. Images of root systems were acquired without manual intervention, over extended periods, using multiple scanners controlled by customized software. Mixed-effects models were used to describe the sources of variation in root traits contributing to root system architecture estimated from digital images. It was calculated that between one and 43 replicates would be required to detect a significant difference (95% CI 50% difference between traits). Broad-sense heritability was highest for shoot biomass traits (>0.60), intermediate (0.25–0.60) for the length and diameter of primary roots and lateral root branching density on the primary root, and lower (<0.25) for other root traits. Models demonstrate that root traits show temporal variations of various types. The phenotyping platform described here can be used to quantify environmental and temporal variation in traits contributing to root system architecture in B. rapa and can be extended to screen the large populations required for breeding for efficient resource acquisition.

show abstract

Section: Discussionsupporting

confidence: 61%

A scanner system for high-resolution quantification of variation in root growth dynamics of Brassica rapa genotypes

Adu

Chatot

Wiesel

et al. 2014

Journal of Experimental Botany

106

View full text Add to dashboard Cite

show abstract

“…Among them, the B3-DPB gene was consistently identified for all the traits analyzed in present analysis and germination under salt stress in the previous study. Comparable result was also obtained in M. truncatula (Arraouadi et al, 2012) where most of loci associated with salt related traits were located on chromosome 1. However, markers identified on chromosomes 5 and 6 in M. sativa by the present study were not reported in M. truncatula (Arraouadi et al, 2011, 2012).…”

Section: Discussionsupporting

confidence: 61%

“…Numerous reports have been made on plant responses to salt stress and genes involved in salt tolerance have been identified and characterized in this species (See Rose, 2008 for review). QTLs for salt tolerance have been identified on chromosomes 1 and 8 in M. truncatula using recombinant inbred lines derived from a cross between the tolerant and susceptible parents (Arraouadi et al, 2011, 2012). However, limit report has been made on the genetics of the cultivated alfalfa.…”

Section: Discussionmentioning

confidence: 99%

“…QTLs associated with biomass (Robins et al, 2007b), morphological traits (Robins et al, 2007a), winter hardiness, persistence (Brouwer et al, 2000; Alarcón-Zúñiga et al, 2004), self-fertility (Robins and Brummer, 2010), Stagonospora root and crown rot resistance (Musial et al, 2007), anthracnose resistance (Mackie et al, 2007) and aluminum tolerance (Khu et al, 2010) have been identified in alfalfa. QTLs associated with salt tolerance have been identified in the model legume, M. truncatula and mapped to all chromosomes except chromosomes 5 and 6 (Arraouadi et al, 2011, 2012). In addition, a number of salt-responsive genes have recently been identified and characterized in alfalfa (Jin et al, 2010; Soto et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Association Mapping of Loci Associated with Plant Growth and Forage Production under Salt Stress in Alfalfa (Medicago sativa L.)

Liu

2017

Front. Plant Sci.

View full text Add to dashboard Cite

Salinity tolerance is highly desirable to sustain alfalfa production in marginal lands that have been rendered saline. In this study, we used a diverse panel of 198 alfalfa accessions for mapping loci associated with plant growth and forage production under salt stress using genome-wide association studies (GWAS). The plants were genotyped using genotyping-by-sequencing (GBS). A greenhouse procedure was used for phenotyping four agronomic and physiological traits affected by salt stress, including dry weight (DW), plant height (PH), leaf chlorophyll content (LCC), and stomatal conductance (SC). For each trait, a stress susceptibility index (SSI) was used to evaluate plant performance under stressed and non-stressed conditions. Marker-trait association identified a total of 42 markers significantly associated with salt tolerance. They were located on all chromosomes except chromosome 2 based on the alignment of their flanking sequences to the reference genome (Medicago truncatula). Of those identified, 13 were associated with multiple traits. Several loci identified in the present study were also identified in previous reports. BLAST search revealed that 19 putative candidate genes linked to 24 significant markers. Among them, B3 DNA-binding protein, Thiaminepyrophosphokinase and IQ calmodulin-binding motif protein were identified among multiple traits in the present and previous studies. With further investigation, these markers and candidates would be useful for developing markers for marker-assisted selection in breeding programs to improve alfalfa cultivars with enhanced tolerance to salt stress.

show abstract

“…By now, salinity has become a main abiotic stress limiting crop production worldwide, affecting nearly 40 percent of agricultural lands located in arid and semi-arid areas (Mittler 2006; Roy et al 2014). Although many signaling components and molecular mechanisms have been identified which largely extend our understanding of plant tolerance against salt stress, the utilization of the knowledge in crop improvement leaves much to be desired since salt stress induces the genetic and physiological changes at all levels of organization in plants and the salinity tolerance, in common with other environmental stresses, relies on the interactions among multiple genes (Arraouadi et al 2012; Cortina and Culiáñez-Macià 2005; Flowers and Colmer 2015). Simply expressing one or several genes usually has no obvious improvement on the salt tolerance of transgenic crops.…”

Section: Introductionmentioning

confidence: 99%

Two conserved oligosaccharyltransferase catalytic subunits required for N-glycosylation exist in Spartina alterniflora

et al. 2015

View full text Add to dashboard Cite

BackgroundAsparagine (N)-linked glycosylation is one of the most crucial post-translational modifications, which is catalyzed in the lumen of the endoplasmic reticulum (ER) by the oligosaccharyltransferase (OST) in eukaryotic cells. Biochemical and genetic assay leads to the identification of the nine subunits (Ost 1–6, Stt3, Swp1 and Wbp1) of the yeast OST and in which Stt3p is proposed playing a central and conserved role in N-glycosylation. Two STT3 isoform genes, STT3A and STT3B, exist in the plant and mammal genomes. OST with different catalytic STT3 isoforms has different enzymatic properties in mammals. The mutation of STT3A in Arabidopsis thaliana causes a salt hypersensitive phenotype the inhibited root growth and swollen root tips suggesting protein N-glycosylation is indispensable for plant growth and development. Spartina alterniflora is widely used for shoreline protection and tidal marsh restoration due to the strong salt tolerance although the exact molecular mechanism is little known. To explore the possible biological roles of N-glycosylation in plant adaptive resistance to salinity stress, we cloned the STT3 genes from S. alterniflora and heterogenously expressed them in Arabidopsis mutant to observe the functional conservation.Results SaSTT3A and SaSTT3B genes were cloned from Spartina alterniflora. SaSTT3A genomic sequences spanned over 23 exons and 22 introns, while SaSTT3B had 6 exons and 5 introns. The gene structures of both genes were conserved among the analyzed plant species. Subcellular localization and transmembrane structure prediction revealed that these two genes had 13 and 11 transmembrane helices respectively. The functional complementation in which the cDNA of SaSTT3A and SaSTT3B driven by CaMV 35S promoter completely or partially rescued Arabidopsis stt3a-2 mutant salt-sensitive phenotype, indicating STT3A functions conservatively between glycophyte and halophyte and N-glycosylation might be involved in plant resistance to salinity.ConclusionsTwo STT3 isoform genes, SaSTT3A and SaSTT3B, were cloned from S. alterniflora and they were evolutionally conserved at gene structure and coding sequences compared with their counterparts. Moreover, SaSTT3 genes could successfully rescue Arabidopsis stt3a-2 salt-sensitive phenotype, suggesting there exists a similar N-glycosylation process in S. alterniflora. Here we provided a first piece of evidence that the N-glycosylation might be involved in salt tolerance of halophyte.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-015-0111-9) contains supplementary material, which is available to authorized users.

show abstract

QTL mapping of physiological traits associated with salt tolerance in Medicago truncatula Recombinant Inbred Lines

Cited by 46 publications

References 42 publications

A scanner system for high-resolution quantification of variation in root growth dynamics of Brassica rapa genotypes

A scanner system for high-resolution quantification of variation in root growth dynamics of Brassica rapa genotypes

Genome-Wide Association Mapping of Loci Associated with Plant Growth and Forage Production under Salt Stress in Alfalfa (Medicago sativa L.)

Two conserved oligosaccharyltransferase catalytic subunits required for N-glycosylation exist in Spartina alterniflora

Contact Info

Product

Resources

About