QTL mapping in Fusarium graminearum identified an allele of FgVe1 involved in reduced aggressiveness

Laurent, Benoit; Moinard, Magalie; Spataro, Cathy; Chéreau, Sylvain; Zehraoui, Enric; Blanc, Richard; Lasserre, Pauline; Ponts, Nadia; Foulongne-Oriol, M.

doi:10.1016/j.fgb.2021.103566

Cited by 3 publications

(2 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While fungal pathogens can defeat plant resistance barriers because of aggressiveness shifts due to mutation, immigration or sexual recombination (McDonald and Linde, 2002;Sakr, 2023), such variation in aggressiveness observed herein can be ascribed to mutation and/or sexual recombination. A new mutation was identified and functionally validated in the gene FgVe1, coding for a velvet protein known to be involved in pathogenicity and secondary metabolism production in several fungi (Laurent et al, 2021). The high-gene flow suggests potential to create pathogen populations that can rapidly adapt to management strategies like fungicide applications and resistant cultivars (Talas and McDonald, 2015).…”

Section: Resultsmentioning

confidence: 99%

Adaptation of Fusarium Species causing Head Blight to Quantitative Resistance in Wheat: Field Evidence for Increased Aggressiveness in a New Pathogen Population

Sakr

2023

Int. J. Phytopathol.

View full text Add to dashboard Cite

The use of quantitatively resistant wheat cultivars is an essential component of a sustainable management strategy of Fusarium head blight (FHB), caused by several Fusarium species. However, little information is available on the variation of aggressiveness of the newly emerging FHB collection compared to old one. It is therefore important to determine to what extent FHB populations can be selected for increased aggressiveness by wheat cultivars with several levels of quantitative resistance. To this end, FHB populations were sampled in 2005 (old population) and in 2015 (new population) from one of the major Syrian wheat production regions, chosen as a location where head blight occurs regularly. New and old FHB isolates were characterized for aggressiveness by single-floret inoculation under controlled conditions on eight durum and bread wheat cultivars of contrasting susceptibility to FHB, and molecularly distinguished using DNA markers. Results showed the new population caused a higher disease severity (ranging from 55% to 67%) than the old population. Thus, their aggressiveness increased between early and late samplings, suggesting that wheat plants cultivated over 10 years selected for increased aggressiveness during epidemics. Our comparative population genetic analyses with analyzed markers showed that the new population had more polymorphic loci compared with the old one. The information obtained in this study indicated that FHB populations adapt to prevailing wheat cultivars, irrespective of their resistance levels, and can therefore overcome polygenic, quantitative resistance. Adaptation to wheat resulting in increased pathogen aggressiveness that was not specific may render quantitative resistance nondurable if not properly managed

show abstract

Section: Resultsmentioning

confidence: 99%

Adaptation of Fusarium Species causing Head Blight to Quantitative Resistance in Wheat: Field Evidence for Increased Aggressiveness in a New Pathogen Population

Sakr

2023

Int. J. Phytopathol.

View full text Add to dashboard Cite

show abstract

“…In addition to classic QTLs, traits have also been reported to be impacted in yeasts by aneuploidy (Todd, et al 2017) and genomic rearrangements (Zimmer et al 2014). QTL mapping has also been successfully used in A. bisporus, for identifying the genomic regions controlling color and spore number (Foulongne-Oriol et al 2012;Imbernon et al 1996), in the causal agent of the cereal disease Fusarium head blight, Fusarium graminearum, for identifying the genetic determinants of aggressiveness (Laurent et al 2021), and in the Lachancea waltii yeast, for identifying genomic regions controlling the growth rate in the presence of various drugs (Peltier et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Generation of diversity in the blue cheese moldPenicillium roquefortiand identification of pleiotropic QTL for key cheese-making phenotypes

Caron,

Crequer,

Le Piver

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Elucidating the genomic architecture of quantitative traits is essential for our understanding of adaptation and for breeding in domesticated organisms.Penicillium roquefortiis the mold used worldwide for the blue cheese maturation, contributing to flavors through proteolytic and lipolytic activities. The two domesticated cheese populations display very little genetic diversity, but are differentiated and carry opposite mating types. We produced haploid F1 progenies from five crosses, using parents belonging to cheese and non-cheese populations. Analyses of high-quality genome assemblies of the parental strains revealed five large translocations, two having occurred via a circular intermediate. Offspring genotyping with genotype-by-sequencing (GBS) revealed several genomic regions with segregation distortion, possibly linked to degeneration in cheese lineages. We found transgressions for several traits relevant for cheese making, with offspring having more extreme trait values than parental strains. We identified quantitative trait loci (QTLs) for colony color, lipolysis, proteolysis, extrolite production, including mycotoxins, but not for growth rates. Some genomic regions appeared rich in QTLs for both lipid and protein metabolism, and other regions for the production of multiple extrolites, indicating that QTLs have pleiotropic impacts. Some QTLs corresponded to known biosynthetic gene clusters, e.g., for the production of melanin or extrolites. F1 hybrids constitute valuable strains for cheese producers, with new traits and genetic diversity, and allowed identifying target genomic regions for traits important in cheese making, paving the way for strain improvement. The findings further contribute to our understanding of the genetic mechanisms underlying rapid adaptation, revealing convergent adaptation targeting major regulators.

show abstract

Erosion of Quantitative Resistance in Wheat and Barley to fusarium Head Blight: Gene Pyramiding Achieves and Durability Study

Sakr¹

2022

TOASJ

View full text Add to dashboard Cite

Background: Fusarium head blight (FHB) caused by several Fusarium species, is one of the diseases causing the greatest worldwide damage to small grain cereals, especially wheat and barley. FHB outbreaks can substantially diminish grain yield and end-use quality due to sterile florets and withered, mycotoxin-contaminated grain kernels. Great effort has been accomplished to combat FHB in the past decades; however, solutions to prevent FHB damage are limited. The development of quantitative resistant cultivars is considered a sustainable and highly desired approach to reducing FHB damage. Objective: This review aims to combine novel data related to the potential ability of pathogens to evolve aggressiveness, erosion of quantitative head blight resistance in wheat and barley, and applying gene pyramiding which enhances host resistance to FHB infection to achieve durable head blight resistance. Results: Although FHB-resistance resources have been successfully utilized by resistant parents in wheat and barley breeding programs globally, this policy does not ensure high resistance to FHB since resistance will erode due to aggressiveness shifts of head blight population. The increasing practice of monoculture wheat and barley cultivation has perhaps raised the rate of head blight pathogen evolution and obligated the natural balance shifting in favor of the pathogen, creating more repeated and grave epidemics, even in provinces where FHB has not been earlier recorded. The emergence of more aggressive FHB populations has been observed in the field and under experimental laboratory conditions. It suggests adaptation followed by a spread of some strains in their environment, including adaptation to FHB-resistant breeds and possible erosion of wheat and barely resistance. Therefore, the pyramiding of several QTLs with high impact in one cereal cultivar may extend durability. Conclusion: If a pyramiding of multiple resistances improving QTL combined with selection against suspected susceptibility factors is achieved in novel cultivars, the evolution of FHB pathogens might be slowed owing to reduced exposure to the pathogen, disruptive selection on FHB populations and subsequently reduced fitness of Fusarium fungi. This would stabilize the pathogen population and contribute to the durability of FHB resistance.

show abstract

QTL mapping in Fusarium graminearum identified an allele of FgVe1 involved in reduced aggressiveness

Cited by 3 publications

References 80 publications

Adaptation of Fusarium Species causing Head Blight to Quantitative Resistance in Wheat: Field Evidence for Increased Aggressiveness in a New Pathogen Population

Adaptation of Fusarium Species causing Head Blight to Quantitative Resistance in Wheat: Field Evidence for Increased Aggressiveness in a New Pathogen Population

Generation of diversity in the blue cheese moldPenicillium roquefortiand identification of pleiotropic QTL for key cheese-making phenotypes

Erosion of Quantitative Resistance in Wheat and Barley to fusarium Head Blight: Gene Pyramiding Achieves and Durability Study

Contact Info

Product

Resources

About