QTL Mapping for Gray Leaf Spot Resistance in a Tropical Maize Population

Liu, L.; Zhang, Y. D.; Li, H. Y.; Bi, Yaqi; Yu, Lijuan; Fan, Xing; Tan, Jing; Jeffers, Daniel; Kang, Manjit S.

doi:10.1094/pdis-08-14-0825-re

Cited by 28 publications

(40 citation statements)

References 46 publications

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“…(), and Liu et al. (). qRgls.CH‐3 was located in bins 3.04‐3.05, which overlapped with the disease resistance QTL intervals that were mapped by Bubeck et al.…”

Section: Discussionmentioning

confidence: 99%

“…Liu et al. () detected a total of seven resistance QTLs, but only one was detected in two environments. Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

“…Identification of GLS resistance was conducted mainly through natural inoculation. Luding County in Sichuan Province and Baoshan City in Yunnan Province are the main epidemic areas of maize GLS caused by C. zeina and are ideal areas for natural inoculation (Liu et al., ; Zhang et al., ; Zhao et al., ). From 2014 to 2015, the parental lines, 446 and 08‐641, the F 1 generation, and 233 F 2:3 families were planted in experimental fields in Luding (1,950 m above sea level) and Baoshan (1,850 m above sea level) for GLS resistance identification.…”

Section: Methodsmentioning

confidence: 99%

“…Liu et al. () constructed an F 2 population with Y32 × Ye478 and detected QTLs on chromosomes 2, 3, 4, 5, and 8 that were related to GLS resistance and explained 2.9%‐19.9% of the phenotypic variation. Lennon, Krakowsky, Goodman, Flint‐Garcia, and Balint‐Kurti () obtained RILs by hybridizing teosinte with B73 for resistance QTL mapping and found that resistance genes were located in bins 2.04, 3.06, 4.07, 5.03, 8.06, and 9.03.…”

Section: Introductionmentioning

confidence: 99%

“…() used meta‐analysis methods to integrate the QTL mapping results of five GLS resistance studies and identified 26 “real” QTLs and seven hotspots in bins 1.06, 2.06, 3.04, 4.06, 4.08, 5.03, and 8.06. In addition to directly locating maize GLS resistance QTLs, attempts were made to locate QTLs related to the control of flowering time, and a close connection was detected between the two traits (Berger et al., ; Liu et al., ).…”

Section: Introductionmentioning

confidence: 99%

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QTL mapping for resistance of maize to grey leaf spot

Lin

Leng

et al. 2017

Journal of Phytopathology

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Grey leaf spot (GLS) is a global maize leaf disease that seriously endangers maize production. Discovering and utilizing genetic loci for GLS resistance would be useful for breeding new varieties with improved resistance. In this study, 233 F2:3 families (produced from the susceptible inbred line 08‐641 × the resistant inbred line 446) were used for quantitative trait locus (QTL) mapping of resistance to GLS. Five GLS resistance QTLs were detected on chromosomes 1, 2, 3, 4, and 6, which explained 6.7%‐21.3% of the phenotypic variation. The QTLs, qRgls.CH‐4, qRgls.CH‐1, qRgls.CH‐2, and qRgls.CH‐6, were stably expressed in the four environments, and all loci for GLS resistance were derived from the resistant parent, 446. The additive effects of qRgls.CH‐4, qRgls.CH‐1, and qRgls.CH‐6 were significantly greater than their single dominant effects, which may be beneficial for GLS resistance breeding. The QTL qRgls.CH‐6, located in bins 6.02–6.05, did not overlap with any previously reported resistance QTL and thus was identified here for the first time. QTL analysis of PI (leaf performance index) detected three leaf function QTLs on chromosomes 4, 8, and 9 were related to GLS resistance and explained 4.8%‐6.2% of the phenotypic variation. Among them, qPI.CH‐4 was significantly stronger expressed in several environments; this allele associated with increased leaf function came from the resistant parent, 446, and its interval overlapped with that of qRgls.CH‐4. Furthermore, both qRgls.CH‐4 and qPI.CH‐4 were located in a hotspot area for GLS resistance in bins 4.05‐4.06, indicating that GLS resistance was significantly related to leaf performance and that GLS significantly reduced leaf photosynthetic performance.

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“…(), and Liu et al. (). qRgls.CH‐3 was located in bins 3.04‐3.05, which overlapped with the disease resistance QTL intervals that were mapped by Bubeck et al.…”

Section: Discussionmentioning

confidence: 99%

“…Liu et al. () detected a total of seven resistance QTLs, but only one was detected in two environments. Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

QTL mapping for resistance of maize to grey leaf spot

Lin

Leng

et al. 2017

Journal of Phytopathology

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Putative genes for resistance to gray leaf spot of maize based on genomic resequencing using recombinant inbred lines

Liu

et al. 2021

Crop Science

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Gray leaf spot (GLS) of maize (Zea mays L.), caused by Cercospora spp., inflicts serious yield losses. Four recombinant inbred lines (RILs) were developed from the [(YML32×Q11) × Q11] backcross population. Two flanking markers, GZ204 and IDP5, defining quantitative trait locus (QTL) qRgls1 for resistance to GLS, were detected in three RILs (RL1_1, RL1_2, and RL2_1) but not in RL2_2. This QTL is located on chromosome 8 in bin 8.02. The field resistance of RL2_2 was, however, similar to that of RL1_1 and RL1_2. The RL2_2 was found to possess a unique DNA segment (Zm00001d008467) that was not found in the other three RILs. We tested the hypothesis that Zm00001d008467 is a candidate gene associated with resistance to GLS. Sequencing showed that clean read counts for RL1_1, RL1_2, RL2_1, and RL2_2 were different and that single‐nucleotide polymorphisms (SNPs)/Exonic SNPs in RL1_1, RL1_2, RL2_1, and RL2_2 were 4908618/14042, 2423243/10668, 4512193/12577, and 2321974/6731, respectively. Results revealed that one of the three exclusive extron nonsynonymous SNPs (Zm00001d008467_T003) had caused a change in a protein that is a wall‐associated non–arginine‐aspartate (non‐RD) receptor‐like kinase. This protein is suspected to trigger resistance to GLS. Our results confirm that Zm00001d008467 is a candidate gene, located in chromosome 8 in bin 8.02, that is associated with GLS resistance. We concluded that the exclusive extron nonsynonymous SNPs in the QTL that had changed the structure and function of the coding protein was responsible for the GLS resistance in RIL2_2 in the absence of resistance QTL qRgls1.

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