QTL mapping for benzoxazinoid content, preharvest sprouting, α-amylase activity, and leaf rust resistance in rye (Secale cereale L.)

Milczarski, Paweł; Masojć, Piotr; Krajewski, Paweł; Stochmal, Anna; Kowalczyk, Mariusz; Angelov, Mihail; Ivanova, Valentina; Schollenberger, M.; Wakuliński, W; Banaszak, Zofia; Banaszak, Katarzyna; Rakoczy-Trojanowska, Monika

doi:10.1371/journal.pone.0189912

Cited by 15 publications

(23 citation statements)

References 33 publications

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“…ScGAMYB is located within a near-centromeric region on chromosome 3R where QTL for α-amylase activity were found in rye [17,19,21]. As expected, analysis performed here indicated significant relationship between ScGAMYB allelic segregation and α-amylase activity in grain.…”

Section: Discussionsupporting

confidence: 82%

GAMYB gene in rye – sequence, polymorphisms, map location, allele-specific markers and relationship with selected agronomic traits

Bienias¹,

Góralska²,

Masojć³

et al. 2019

Preprint

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Background. A master GA-induced regulatory protein, crucial for development and germination of cereal grain and involved in anther formation is MYB transcription factor GAMYB, activating a vast number of genes including high-molecular-weight glutenin and α-amylase gene families. This paper presents the first attempt to characterize rye gene encoding GAMYB in relation to its sequence, polymorphisms and phenotypic effects. Results. ScGAMYB gene was identified and mapped on rye chromosome 3R using high-density DArT/DArTseq-based maps developed in two mapping populations. Comparative analysis of the gene sequence revealed its high level of homology to wheat and barley orthologues. Single nucleotide polymorphisms detected among rye inbred lines allowed developing AS-PCR markers for ScGAMYB (ten pairs of primers) which might be used to detect this gene in wide genetic stocks of rye and triticale. Segregation of ScGAMYB alleles showed significant relationship with quantitative traits including plant height, thousand grain weight, α-amylase activity, earliness per se and leaf rolling. Conclusions. The research showed the strong similarity of rye GAMYB sequence to its orthologues in other Graminae and confirmed the position in the genome consistent with the collinearity rule of cereal genomes. The statistically significant, however moderate association of ScGAMYB with many agronomic features has been pointed out, which proved that this gene is a QTL of pleiotropic character. The effect of ScGAMYB on flowering time was statistically the most significant. Developed sequence-based, allele-specific PCR markers could be useful in research and application purposes.

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Section: Discussionsupporting

confidence: 82%

GAMYB gene in rye – sequence, polymorphisms, map location, allele-specific markers and relationship with selected agronomic traits

Bienias¹,

Góralska²,

Masojć³

et al. 2019

Preprint

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“…Close linkage between these QTL and the dwarfing gene may result in association of grain parameters with plant height, which means that allele compositions in these loci in a short-straw donor line is crucial for the agronomic performance of developed progenies. The considered chromosome 3R region was shown to contain QTL controlling benzoxazinoid content and leaf rust resistance [ 26 ] which in turn raises the question about the response of Ddw4 to brassinosteroids and its association with disease resistance. Ddw4 may also be associated with preharvest sprouting resistance (PHS) and alpha-amylase activity in the grain (AA), as it is tightly linked with QTL for these traits [ 27 – 30 ] and with GA 3 oxidase [ 31 ], a candidate gene for PHS.…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel, dominant dwarfing gene (Ddw4) and its effect on morphological traits of rye

et al. 2018

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Shortening rye stems to improve lodging resistance is among the major tasks awaiting breeders of this cereal. The most straightforward way to achieve this goal is the implementation of a dominant dwarfing gene into high yielding cultivars. The choice of dominant dwarfing genes in rye is limited to Ddw1 and Ddw3 loci, which are well characterized with respect to map position and tightly linked molecular markers on the long arms of chromosomes 5RL and 1RL, respectively. This paper reports on the identification and preliminary characterization of a novel dominant dwarfing gene, Ddw4, from line S44. This was mapped within the centromeric region of chromosome 3R. The Ddw4 gene is sensitive to exogenous gibberellin. Its introduction into the rye populational cultivar Dańkowskie Amber decreased plant height by c. 54% without any negative effects on spike length and number of kernels per spike. Further genetic studies are needed to determine the perspectives for application of the newly detected dwarfing gene into breeding programs for short-stem rye.

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“…The current strategies to mitigate, and if possible eliminate LMA revolve around efforts to understand the genetic basis of LMA ( Mrva and Mares, 2001 ; Mohler et al, 2014 ; Mieog et al, 2017 ; Milczarski et al, 2017 ; Borner et al, 2018 ). These LMA strategies have been put in place despite a clear knowledge gap around the real impact of LMA on wheat flour end product quality.…”

Section: Introductionmentioning

confidence: 99%

Does Late Maturity Alpha-Amylase Impact Wheat Baking Quality?

et al. 2018

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Late maturity α-amylase (LMA) and pre-harvest sprouting (PHS) are both recognized as environmentally induced grain quality defects resulting from abnormally high levels of α-amylase. LMA is a more recently identified quality issue that is now receiving increasing attention worldwide and whose prevalence is now seen as impeding the development of superior quality wheat varieties. LMA is a genetic defect present in specific wheat genotypes and is characterized by elevated levels of the high pI TaAMY1 α-amylase, triggered by environmental stress during wheat grain development. TaAMY1 remains present in the aleurone through the harvest, lowering Falling Number (FN) at receival, causing a down-grading of the grain, often to feed grade, thus reducing the farmers’ income. This downgrading is based on the assumption within the grain industry that, as for PHS, a low FN represents poor quality grain. Consequently any wheat line possessing low FN or high α-amylase levels is automatically considered a poor bread wheat despite there being no published evidence to date, to show that LMA is detrimental to end product quality. To evaluate the validity of this assumption a comprehensive evaluation of baking properties was performed from LMA prone lines using a subset of tall non-Rht lines from a multi-parent advanced generation inter-cross (MAGIC) wheat population grown at three different sites. LMA levels were determined along with quality parameters including end product functionality such as oven spring, bread loaf volume and weight, slice area and brightness, gas cell number and crumb firmness. No consistent or significant phenotypic correlation was found between LMA related FN and any of the quality traits. This manuscript provides for the first time, compelling evidence that LMA has limited impact on bread baking end product functionality.

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QTL mapping for benzoxazinoid content, preharvest sprouting, α-amylase activity, and leaf rust resistance in rye (Secale cereale L.)

Cited by 15 publications

References 33 publications

GAMYB gene in rye – sequence, polymorphisms, map location, allele-specific markers and relationship with selected agronomic traits

GAMYB gene in rye – sequence, polymorphisms, map location, allele-specific markers and relationship with selected agronomic traits

Identification of a novel, dominant dwarfing gene (Ddw4) and its effect on morphological traits of rye

Does Late Maturity Alpha-Amylase Impact Wheat Baking Quality?

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