QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli

Abstract

Italian common wheat cultivars Libellula and Strampelli, grown for over three decades in Gansu province of China, have shown effective resistance to stripe rust. To elucidate the genetic basis of the resistance, F(3) populations were developed from crosses between the two cultivars and susceptible Chinese wheat cultivar Huixianhong. The F(3) lines were evaluated for disease severity in Beijing, Gansu and Sichuan from 2005 to 2008. Joint- and single-environment analyses by composite interval mapping identified five quantitative trait loci (QTLs) in Libellula for reduced stripe rust severity, designated QYr.caas-2DS, QYr.caas-4BL, QYr.caas-5BL.1, QYr.caas-5BL.2 and QYr.caas-7DS, and explained 8.1-12.4, 3.6-5.1, 3.4-8.6, 2.6 and 14.6-35.0%, respectively, of the phenotypic variance across four environments. Six interactions between different pairs of QTLs explained 3.2-7.1% of the phenotypic variance. The QTLs QYr.caas-4BL, QYr.caas-5BL.1 and QYr.caas-7DS were also detected in Strampelli, explaining 4.5, 2.9-5.5 and 17.1-39.1% of phenotypic variance, respectively, across five environments. Three interactions between different pairs of QTLs accounted for 6.1-35.0% of the phenotypic variance. The QTL QYr.caas-7DS flanked by markers csLV34 and Xgwm295 showed the largest effect for resistance to stripe rust. Sequence analyses confirmed that the lines with the QYr.caas-7DS allele for resistance carried the resistance allele of the Yr18/Lr34 gene. Our results indicated that the adult-plant resistance gene Yr18 and several minor genes confer effective durable resistance to stripe rust in Libellula and Strampelli.

Identification of adult plant resistance to stripe rust in the wheat cultivar Cappelle-Desprez

“…Camp Rémy, did not identify a QTL on chromosome 5B. Stripe rust resistance QTL on chromosome 5B have been detected in the Italian cvs Libellula and Strampelli (Lu et al 2009), the Israeli cv. Oligoculm (Suenaga et al 2003), the Australian cv.…”

“…A stripe rust resistance QTL was identified in the Italian cv. Libellula, QYr.caas-2DS explaining 8.4-12.1% of the phenotypic variance (Lu et al 2009), while Suenaga et al (2003) detected a stripe rust resistance QTL in this region of 2DS in the cv. Oligoculm which explained less than 10% of the phenotypic variance.…”

“…Depending on environment and disease pressure, a low level of stripe rust resistance was observed for Palmiet, supporting the detection of this QTL. QTL for stripe rust APR have been reported on chromosome 4B in the cvs Alcedo (Jagger et al 2011), Oligoculm (Suenaga et al 2003), Avocet S (William et al 2006), Guardian (Melichar et al 2008) and cvs Libellula and Strampelli (Lu et al 2009). The stripe rust QTL from Avocet S was also reported to have an effect on leaf rust (William et al 2006).…”

Genetic purity assessment of indica rice hybrids through DNA fingerprinting and grow out test

“…Molecular markers, in contrast, offer an impartial means for precise molecular profiling of -1 varieties/hybrids and their purity assessment in short span of time (Rehem , 2010). Microsatellite (SSR/ STMS) marker based high level of polymorphism or fingerprinting assays have now emerged as one of the new and rapid technology for cultivar/ impurity identification to replace/ supplement the conventional grow-out test grow out test (Yashitola , 2002;Yifru ., 2006;Lu ., 2009;Bora 2016). Cytoplasmic male sterile (CMS-A line), maintainer (B-line), restorer (R-line) lines and their hybrids have been screened by a number of microsatellite and sequence tagged site markers by several workers (Sundaram 2008 ;Jaikishan , 2010; Therefore, keeping in view of the above facts, the objective of the present study was to estimate and identify the level of purity in seed lots of nine rice hybrids with their parental lines through microsatellite markers based DNA fingerprints and grow out test, and also to establish the basis for identification, authentication, protection and monitoring of seed purity for hybrids evaluated.…”

Durable resistance to the wheat rusts: integrating systems biology and traditional phenotype-based research methods to guide the deployment of resistance genes

“…In the last two years alone, more than 20 QTL conferring some degree of quantitative resistance to wheat stripe rust were reported (Cheng and Chen 2008;Chhuneja et al 2008;Guo et al 2008;Melichar et al 2008;Rosewarne et al 2008;Santra et al 2008;Carter et al 2009;Dedryver et al 2009;Lu et al 2009). These QTL present breeders with the possibility of over 1,000 unique three-QTL combinations, a number far beyond the realm of investigation.…”