QTL editing confers opposing yield performance in different rice varieties

Shen, Lan; Chün, Wang; Fu, Yaping; Wang, Junjie; Liu, Qing; Zhang, Xiaoming; Yan, Chuan; Qian, Qian

doi:10.1111/jipb.12501

Cited by 126 publications

(62 citation statements)

References 13 publications

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“…Recent examples in rice showcase this approach, where combining mutations in two negative regulators of grain size (GS3; Fan et al ) and grain number (GN1a; Ashikari et al ) increased grain size and number across all ten genotypes tested (Shen et al ). However, these positive effects only translated into yield increases for three of the ten cultivars.…”

Section: Interactionsmentioning

confidence: 99%

A reductionist approach to dissecting grain weight and yield in wheat

Brinton

Uauy

2019

JIPB

123

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Grain yield is a highly polygenic trait that is influenced by the environment and integrates events throughout the life cycle of a plant. In wheat, the major grain yield components often present compensatory effects among them, which alongside the polyploid nature of wheat, makes their genetic and physiological study challenging. We propose a reductionist and systematic approach as an initial step to understand the gene networks regulating each individual yield component. Here, we focus on grain weight and discuss the importance of examining individual sub‐components, not only to help in their genetic dissection, but also to inform our mechanistic understanding of how they interrelate. This knowledge should allow the development of novel combinations, across homoeologs and between complementary modes of action, thereby advancing towards a more integrated strategy for yield improvement. We argue that this will break barriers in terms of phenotypic variation, enhance our understanding of the physiology of yield, and potentially deliver improved on‐farm yield.

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Section: Interactionsmentioning

confidence: 99%

A reductionist approach to dissecting grain weight and yield in wheat

Brinton

Uauy

2019

JIPB

123

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“…В дальнейшем у семи сортов риса были получены нокаутные генотипы по GS3, а также двойные нокауты Gn1a + GS3. Несмотря на то что у растений, нокаутных по GS3, было увеличено зерно, а у двойных нокаутов еще и повышена озерненность, продуктивность повышалась (на 3-7 %) лишь у трех полученных вариантов, тогда как у семи -неожиданно снижалась вследствие уменьшения числа продуктивных побегов (Shen et al, 2016).…”

Section: Plant Genetics Vavilov Journal Of Genetics and Breeding • 21unclassified

“…Гены сельскохозяйственных растений, модифицированные с помощью системы CRISPR/Cas достичь 100 % частоты получения мутантных растений (Shen et al, 2016;Xu et al, 2016). Следует отметить, что в большинстве представленных работ продемонстрирована возможность получения мо дифицированных растений, свободных от служебных кон струкций (transgene clean или transgenefree) (см.…”

Section: генетика растенийunclassified

Crop genes modified using CRISPR/Cas system

Korotkova¹,

Gerasimova²,

Шумный³

et al. 2017

Vestn. VOGiS

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The CRISPR/Cas system is the most promising among genome editing tools. It can provide the development of modified nontransgenic plants with the possibility of simultaneous multiple targeted mutations. The purpose of this review is to analyze published papers describing the utilization of the CRISPR/Cas system for crop gene modification in order to assess the potential of this technology as a new plant breeding technique. The search for "CRISPR & crop name" within article titles, abstracts and keywords in the Scopus database was carried out for 45 crops. Among a total of 206 search results, only 88 have been recognized as original articles describing editing crop genes with the CRISPR/Cas system. A total of 145 target genes of 15 crops are described in these 88 articles, including rice with the largest number of genes modified (78 genes). In these studies, the ability to get transgene-free modified plants was widely demonstrated. However, in most cases research was aimed at the approbation of the technology or was to elucidate target gene function, while modification of just 37 target genes was related with crop improvement. We present here a catalogue of these genes. In most of these cases, modifications resulted in knockout of the genes such as negative growth and development regulators or negative regulators of plant resistance. In most cases, the phenotype of modified plants was assessed, and the presence of desired changes was shown. However, since the estimated number of "negative regulators" is limited in plant genomes, the CRISPR-directed gene knockout has a restricted potential for crop improvement. Intensive application of the CRISPR/Cas system for more complicate modifications such as replacement of defect alleles by functional ones or insertion of a desired gene is required (so far reports about such modifications are very rare in crops). In addition, to provide a basis for broad practical application of CRISPR/Cas-based genome editing, more cultivars of crop species should be involved in ongoing studies. Just a few genotypes of crop species have been used for gene modifications thus far. Nevertheless, in spite of the restrictions mentioned, essential success has Система CRISPR/Cas -один из самых перспективных способов геномного редактирования. Этот доступный метод позволяет получать нетрансгенные растения с заданными модификациями, причем можно одновременно производить мутации в несколь-ких мишенях. Цель настоящего обзора -анализ опубликованных работ, в которых система CRISPR/Cas использована для модифика-ции генов сельскохозяйственных растений, с тем чтобы оценить потенциал этой технологии как нового метода селекции рас-тений. Для 45 сельскохозяйственных культур проведен поиск по сочетанию ключевого слова CRISPR с названием культуры (поиск осуществлялся в названиях, аннотациях и ключевых словах статей из журналов, индексируемых в базе данных Scopus). Среди 206 результатов поиска только 88 содержали описание эксперимен-тальных работ, в которых использована система CRISPR/Cas. В этих работах описаны ...

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“…Rice blast and bacterial blight are major destructive diseases that seriously affect not only global rice production but also rice quality. In addition, the disruption of more than one gene using a CRISPR/ Cas9 vector has been reported previously (Shen et al 2016(Shen et al , 2017. Editing multiple genes using the CRISPR/ Cas9 system may become a trend in the future.…”

Section: Introductionmentioning

confidence: 99%

Developing disease‐resistant thermosensitive male sterile rice by multiplex gene editing

Liu

Shen

et al. 2019

JIPB

Self Cite

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Summary High‐quality and disease‐resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system. We found that the transgene‐free homozygous triple tms5/pi21/xa13 mutants obtained in the T1 generation displayed characteristics of thermosensitive genic male sterility (TGMS) with enhanced resistance to rice blast and bacterial blight. Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing, which could significantly accelerate the breeding of sterile lines.

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QTL editing confers opposing yield performance in different rice varieties

Cited by 126 publications

References 13 publications

A reductionist approach to dissecting grain weight and yield in wheat

A reductionist approach to dissecting grain weight and yield in wheat

Crop genes modified using CRISPR/Cas system

Developing disease‐resistant thermosensitive male sterile rice by multiplex gene editing

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