QTL Analysis for Root Protein in a Backcross Family of Cassava Derived from Manihot esculenta ssp flabellifolia

Akinbo, Olalekan; Labuschagne, Maryke; Marín, Jaime Alberto; Ospina, C.; Santos, Luis Guillermo; Barrera, E.; Gutiérrez, J. P.; Ewa, Favour; Okogbenin, Emmanuel; Fregene, Martin A.

doi:10.1007/s12042-012-9095-8

Cited by 9 publications

(4 citation statements)

References 33 publications

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“…However, cassava has a long growth cycle and it is highly heterozygous, and heterozygosity is the reason that an F 1 population segregates. Quantitative trait loci (QTL) mapping has been done in cassava using F 1 and backcross populations, which normally reveal a high level of segregation (Jorge et al, 2000, 2001; Okogbenin and Fregene, 2002, 2003; Fregene et al, 2006; Akinbo et al, 2012). It takes 8 to 10 yr to develop and release a new cassava cultivar.…”

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Molecular Marker Analysis and Validation of Resistance to Cassava Mosaic Disease in Elite Cassava Genotypes in Nigeria

et al. 2012

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Cassava (Manihot esculenta Crantz), a highly heterozygous crop, is devastated by cassava mosaic disease (CMD). The discovery of the CMD2 dominant gene is helpful in the genetic analysis of CMD resistance. Molecular markers for CMD2 gene were used to introgress CMD resistance into Latin American cassava genotypes and validated in the field for 4 yr for stability of resistance conferred by CMD2. Field screening identified 64 Latin American genotypes with stable resistance to CMD. Resistance to CMD of two Nigerian cassava cultivars (TMS 97/2205 and TMS 98/0505) was analyzed with markers and in the field. Molecular data indicated that CMD resistance in the two Nigerian cultivars was mediated by the CMD2 gene. Results showed TMS 97/2205 to be highly resistant to CMD in three ecological zones in Nigeria. Further genetic analysis of this genotype as a source of high level of resistance to CMD using a segregating F1 population derived from a TMS 97/2205 × NR 8083 cross was initiated using 530 simple sequence repeat (SSR) markers to identify quantitative trait loci (QTL) for CMD resistance. A marker (NS198) associated with a QTL for CMD resistance, explaining 11% of the phenotypic variance observed, was identified. The combined effect of this QTL and CMD2 may account for the high level of resistance of TMS 97/2205. The resistance profile of the evaluated CMD2 genotypes in growth cycle was not uniform and was affected by genetic background. The discovery of a new QTL (CMD3) for CMD resistance in TMS 97/2205 offers new opportunities for pyramiding CMD genes for enhanced durability of CMD resistance in cassava.

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Molecular Marker Analysis and Validation of Resistance to Cassava Mosaic Disease in Elite Cassava Genotypes in Nigeria

et al. 2012

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“…In fact, over 20% decline in yield/hectare of storage root was reported between 2007 and 2017, despite a significant increase in the area of land cultivated within this period ( Otekunrin and Sawicka, 2019 ). Factors, such as susceptibility to diseases and high post-harvest loss due to rapid physiological deterioration are major limitations preventing Nigeria from reaching its full potential in cassava production ( Fregene et al, 1997 ; Fregene et al, 2000 ; Akano et al, 2002 ; Ogbe et al, 2006 ; Akinbo et al, 2007 ; Akinbo et al, 2011 ; Akinbo et al, 2012a ; McCallum et al, 2017 ; Zainuddin et al, 2018 ). However, with biotechnology approach, scientists from national agricultural research institutions in Brazil, Nigeria, Ghana and Uganda, are rapidly building resistance to green mite, whitefly, cassava mosaic disease and post-harvest physiological deterioration (PPD) with the support of their counterparts at the International Center for Tropical Agriculture (CIAT) in Colombia ( Fregene et al, 1997 ; Akano et al, 2002 ; Rudi et al, 2010 ; Akinbo et al, 2012b ).…”

Section: Prospect Of Using Plant Biotechnology For Improvement Of Maj...mentioning

confidence: 99%

Genetic engineering and genome editing technologies as catalyst for Africa’s food security: the case of plant biotechnology in Nigeria

Adegbaju,

Ajose,

Adegbaju

et al. 2024

Front. Genome Ed.

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Many African countries are unable to meet the food demands of their growing population and the situation is worsened by climate change and disease outbreaks. This issue of food insecurity may lead to a crisis of epic proportion if effective measures are not in place to make more food available. Thus, deploying biotechnology towards the improvement of existing crop varieties for tolerance or resistance to both biotic and abiotic stresses is crucial to increasing crop production. In order to optimize crop production, several African countries have implemented strategies to make the most of this innovative technology. For example, Nigerian government has implemented the National Biotechnology Policy to facilitate capacity building, research, bioresource development and commercialization of biotechnology products for over two decades. Several government ministries, research centers, universities, and agencies have worked together to implement the policy, resulting in the release of some genetically modified crops to farmers for cultivation and Commercialization, which is a significant accomplishment. However, the transgenic crops were only brought to Nigeria for confined field trials; the manufacturing of the transgenic crops took place outside the country. This may have contributed to the suspicion of pressure groups and embolden proponents of biotechnology as an alien technology. Likewise, this may also be the underlying issue preventing the adoption of biotechnology products in other African countries. It is therefore necessary that African universities develop capacity in various aspects of biotechnology, to continuously train indigenous scientists who can generate innovative ideas tailored towards solving problems that are peculiar to respective country. Therefore, this study intends to establish the role of genetic engineering and genome editing towards the achievement of food security in Africa while using Nigeria as a case study. In our opinion, biotechnology approaches will not only complement conventional breeding methods in the pursuit of crop improvements, but it remains a viable and sustainable means of tackling specific issues hindering optimal crop production. Furthermore, we suggest that financial institutions should offer low-interest loans to new businesses. In order to promote the growth of biotechnology products, especially through the creation of jobs and revenues through molecular farming.

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“…This study showed that early root hypertrophy is a complex trait controlled by multiple genes 11 . Akinbo et al identi ed several QTLs affecting the protein content of cassava roots through QTL analysis in a backcross population 12 . Zou et al constructed an AFSM QTL map by crossing two non-inbred parents, KU50 and SC124 (KS population) to obtain 186 cassava populations, and identi ed 260 candidate QTL genes for cold stress and 301 candidate QTL genes for storing root quality and yield 13,14 .…”

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confidence: 99%

Construction of an ultrahigh-density genetic linkage map for Manihot esculenta Crantz and identification of QTL for root quantity traits

Liu,

Li,

Wang

et al. 2024

Preprint

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Single plant root weight, root number, and root length to width ratio are important agronomic traits that affect cassava yield. To explore the QTL related to quantitative traits such as cassava root weight, this study used Hyper-seq simplified resequencing technology to obtain two hybrid progeny from cassava cultivar SC205 as the female parent and cassava cultiva HB60 and cassava cultiva 18R as the male parent, respectively (of which the HB60 × SC205 progeny is called the A1 population, and the 18R × SC205 is called the A2 population). The genotyping of the two F1 mapping populations was conducted, and a genetic map of the maternal parents of the A1 and A2 populations was constructed by comparing the differences between the parental loci and extracting the same variant loci in the offspring and maternal parents. 18 linkage groups were identified for each parent, consistent with the haploid chromosome number of cassava. On average, each parental map had 6,280 markers, with a marker density ranging from 0.37 cM/marker to 0.43 cM/marker. Using the constructed genetic linkage map, QTL analysis of important quantitative traits such as single plant root weight, root number, and root length to width ratio were conducted, and the main QTLs associated with these traits were identified and candidate genes were identified by combining parental transcriptome data. These results help to further validate the use of hyper-seq technology in cassava breeding and provide new methods for developing future varieties.

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QTL Analysis for Root Protein in a Backcross Family of Cassava Derived from Manihot esculenta ssp flabellifolia

Cited by 9 publications

References 33 publications

Molecular Marker Analysis and Validation of Resistance to Cassava Mosaic Disease in Elite Cassava Genotypes in Nigeria

Molecular Marker Analysis and Validation of Resistance to Cassava Mosaic Disease in Elite Cassava Genotypes in Nigeria

Genetic engineering and genome editing technologies as catalyst for Africa’s food security: the case of plant biotechnology in Nigeria

Construction of an ultrahigh-density genetic linkage map for Manihot esculenta Crantz and identification of QTL for root quantity traits

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