QTL Analysis for Resistance to Colletotrichum Acutatum and Phytophthora Cactorum in Octoploid Strawberry (Fragaria X Ananassa)

Denoyés-Rothan, B.; Lerceteau-Köhler, Estelle; Guérin, G.; Bosseur, S.; Bariac, J.; Martín, Elena García; Roudeillac, P.

doi:10.17660/actahortic.2004.663.19

Cited by 26 publications

(29 citation statements)

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“…Inheritance of resistance to P. cactorum was reported using 50 bi-parental crosses among 20 elite genotypes [16]. Results suggested additive, polygenically inherited resistance, which agreed with several QTLs found in a segregating population [17] and that offspring from crosses could display transgressive segregation with phenotypes covering the scale from highly resistant to very susceptible [15]. This paper describes firstly the effect of the type of plants, tray plants or cold stored plants, on the level of susceptibility to P. cactorum after artificial inoculation.…”

Section: Introductionsupporting

confidence: 67%

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Genetic variation of resistance of the cultivated strawberry to crown rot caused by Phytophthora cactorum

Schafleitner

Bonnet

Pedeprat

et al. 2013

Journal of Berry Research

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Evaluation of strawberry resistance to crown rot caused by Phytophthora cactorum is usually performed on a limited number of genotypes. The major objective of this study was to screen large genetic resources to identify potential parents that can be further used in breeding programs. Plants were inoculated by wounding the crown and placing a mycelium disk on the wound. Firstly, plug and cold stored plants were evaluated for their susceptibility to P. cactorum. Secondly, a total of 107 genotypes was evaluated using cold stored plants. Plug plants were very low affected by the wounding inoculation with P. cactorum whatever the genotype, whereas results obtained using cold stored plants consistently reflected the susceptibility of the genotype to crown rot. By using cold stored plants, we evaluated the susceptibility of 70 varieties and 37 advanced lines. Among the genotypes, we identified varieties such as Cirafine or Cireine with high level of resistance similar to the one of Senga Sengana. These data will be useful for choosing parents in breeding programs and for validation of markers linked to the resistance to P. cactorum.

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Section: Introductionsupporting

confidence: 67%

“…Since the mode of inheritance for resistance to P. cactorum is likely polygenic [16,17], which is suggested by the continuum of level of disease resistance in a range of cultivar, a strategy for the identification of QTLs will be developed. Then, markers flanking the most significant QTLs will be used for validation on germplasm.…”

Section: Discussionmentioning

confidence: 99%

Genetic variation of resistance of the cultivated strawberry to crown rot caused by Phytophthora cactorum

Schafleitner

Bonnet

Pedeprat

et al. 2013

Journal of Berry Research

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“…Quantitative resistance to Phytophthora diseases has also been associated with basal defence (Vega-Sánchez et al 2005; Thomas et al 2007). Accordingly, this study characterises a broad range of effector genes and provides candidates to investigate the basis of quantitative strawberry resistance to P. cactorum (Denoyes-Rothan et al 2004; Shaw et al 2006; Shaw et al 2008; Nellist et al 2018). RxLR effectors are still priority candidates disease related pathogen genes for functional study of strawberry resistance to P. cactorum , particularly homologs of AvrSmira2 characterised avirulence genes.…”

Section: Discussionmentioning

confidence: 99%

“…This highlights the importance of understanding pathogen populations in the field and the associated genetic variation in effector complements. In contrast to these Phytophthora pathosystems, strawberry resistance to P. cactorum appears to be quantitative (partial; Denoyes-Rothan et al 2004; Shaw et al 2006; Shaw et al 2008; Nellist et al 2018), and no race structure has been reported to date. As such, resistance is not determined by a single gene-for-gene recognition, as often associated with RxLR (arginine, any amino-acid, leucine, arginine) effectors (Anderson et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum

Armitage

Lysøe

Nellist

et al. 2018

Preprint

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22The oomycete pathogen Phytophthora cactorum causes crown rot, a major disease of 23 cultivated strawberry. We report the draft genome of P. cactorum isolate 10300, cultured 24 from symptomatic Fragaria x ananassa tissue. Our analysis revealed that there are a large 25 number of genes encoding putative secreted effectors in the genome, including nearly 200 26 RxLR domain containing effectors, 77 Crinklers (CRN) grouped into 38 families and 27 numerous apoplastic effectors, such as phytotoxins (PcF proteins) and necrosis inducing 28 proteins. As in other Phytophthora species, the genomic environment of many RxLR and CRN 29 genes differed from core eukaryotic genes, a hallmark of the two-speed genome. We found 30 genes homologous to known Phytophthora infestans avirulence genes including Avr1, Avr3b, 31 Avr4, Avrblb1 and AvrSmira2 indicating effector sequence conservation between Phytophthora 32 species of Clade 1A and 1C. The reported P. cactorum genome sequence and associated 33 annotations represent a comprehensive resource for avirulence gene discovery in other 34 Phytophthora species from Clade 1 and will facilitate effector informed breeding strategies in 35 other crops. 36 37 38 39 40 The oomycetes are a diverse class of eukaryotic microorganisms that include pathogens of plants, 41 animals and fungi (Simão et al. 2015). The causal agents of plant diseases are well represented in 42 this phylogenetic class, with over 60% of known oomycetes characterised as plant pathogens 43

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“…Quantitative trait loci (QTL) mapping is a routine technique for pinpointing genes controlling complex polygenic traits to specific regions of the genome, through statistical analysis. Previous studies have identified resistance to P. cactorum in the octoploid strawberry and it appears to be under polygenic control 16–18 , with a major locus, FaRPc2 , recently reported on linkage group 7D 19 . Variation in resistance has been also observed in the wild progenitors of F .…”

Section: Introductionmentioning

confidence: 99%

Quantitative Trait Loci ControllingPhytophthora cactorumResistance in the Cultivated Octoploid Strawberry (Fragariaxananassa)

Nellist

Vickerstaff

Sobczyk

et al. 2018

Preprint

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The cultivated strawberry, Fragaria x ananassa (Fragaria spp.) is the most economically important global soft fruit. Phytophthora cactorum, a water-borne oomycete causes economic losses in strawberry production globally. A bi-parental cross of octoploid cultivated strawberry segregating for resistance to P. cactorum, the causative agent of crown rot disease, was screened using artificial inoculation. Multiple resistance quantitative trait loci (QTL) were identified and mapped. Three major effect QTL (FaRPc6C, FaRPc6D and FaRPc7D) explained 36% of the variation observed and in total, the detected QTL explained 86% of the variation observed. There were no epistatic interactions detected between the three major QTLs. Testing a subset of the mapping population progeny against a range of P. cactorum isolates revealed no major differences in host response, however, some lines showed higher susceptibility than predicted, indicating that additional undetected factors may affect the expression of some quantitative resistance loci. Using historic crown rot disease score data from strawberry accessions, a preliminary genome-wide association study of 114 individuals revealed additional loci associated with resistance to P. cactorum. Mining of Fragaria vesca Hawaii 4 v1.1 genome revealed candidate resistance genes in the QTL regions.

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QTL Analysis for Resistance to Colletotrichum Acutatum and Phytophthora Cactorum in Octoploid Strawberry (Fragaria X Ananassa)

Cited by 26 publications

References 0 publications

Genetic variation of resistance of the cultivated strawberry to crown rot caused by Phytophthora cactorum

Genetic variation of resistance of the cultivated strawberry to crown rot caused by Phytophthora cactorum

Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum

Quantitative Trait Loci ControllingPhytophthora cactorumResistance in the Cultivated Octoploid Strawberry (Fragariaxananassa)

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