QTL Analysis for Leaf Gas Exchange in an Apple Progeny Grown Under Atmospheric Constraints

Regnard, J. L.; Segura, Vincent; Merveille, N.; Durel, Charles Eric; Costes, Evelyne

doi:10.17660/actahortic.2009.814.60

Cited by 11 publications

(13 citation statements)

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“…Furthermore, sdNDVI and sdSRPI were strongly intercorrelated and QTLs for these variables co-localized at the middle of the LG08 ( Table 5 ; Supplementary Table S2 ). The location of these QTLs also matched with a QTL zone for traits involved in gas exchange, xylem conductance and fruit production on the STK×GS population ( Regnard et al , 2009 ; Lauri et al , 2011 , Guitton et al , 2012 ). These co-localizations could be explained by an increased capacity of the plant to transport water, carbohydrates and sugar to the growing organs, as suggested by Lauri et al (2011) .…”

Section: Discussionmentioning

confidence: 59%

“…The apple tree population studied consisted of progeny derived from a ‘Starkrimson’×’Granny Smith’ cross, characterized by variability in tree vigour, architectural traits ( Segura et al , 2008 ), biennial bearing ( Guitton et al , 2012 ), hydraulic traits ( Lauri et al , 2011 ) and stomatal regulation in response to vapour pressure deficit ( Regnard et al , 2009 ). In February 2007, four replicates of 122 F1-hybrids and their two parents were grafted onto M9 rootstock and randomly planted in an experimental field at the INRA Melgueil experimental station (Diaphen platform, southeast of France, 43°36ʹ N, 03°58ʹ E).…”

Section: Methodsmentioning

confidence: 99%

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Multispectral airborne imagery in the field reveals genetic determinisms of morphological and transpiration traits of an apple tree hybrid population in response to water deficit

Virlet

Costes

Martinez

et al. 2015

EXBOTJ

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Section: Discussionmentioning

confidence: 59%

Section: Methodsmentioning

confidence: 99%

Multispectral airborne imagery in the field reveals genetic determinisms of morphological and transpiration traits of an apple tree hybrid population in response to water deficit

Virlet

Costes

Martinez

et al. 2015

EXBOTJ

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“…Two hotspot regions were detected on LG8, regardless of irrigation regime or year tested. The corresponding physical position of that hotspot on LG8 was approximately 12.58 to 13.17 Mb for Chr8 near marker CH02g09 in the contig MDC002525.346 (14.30–14.35 Mb) on Chr8, which is involved in QTLs for traits associated with gas exchange [ 21 ], xylem conductance [ 54 ], and fruit production [ 55 ], as well as two visual variables related to canopy structure/biomass production and crop nitrogen status [ 22 ]. Lauri et al suggested that this proximity was due to the increase in capacity to transport water and carbohydrates to developing organs [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, based on dense SNP genetic maps of apple, potential genes for fruit texture, acidity and shape have been revealed, respectively [ 18 – 20 ]. Although researchers have identified QTLs for the indices of photosynthetic gas exchange under atmospheric drought stress, as well as the production of vegetative tissue and fruit under soil water-deficit conditions, the associated genes have not been screened because the density of single sequence repeats (SSRs) on their genetic maps has been insufficient [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Mapping QTLs for water-use efficiency reveals the potential candidate genes involved in regulating the trait in apple under drought stress

et al. 2018

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BackgroundImprovement of water-use efficiency (WUE) can effectively reduce production losses caused by drought stress. A better understanding of the genetic determination of WUE in crops under drought stress has great potential value for developing cultivars adapted to arid regions. To identify the genetic loci associated with WUE and reveal genes responsible for the trait in apple, we aim to map the quantitative trait loci (QTLs) for carbon isotope composition, the proxy for WUE, applying two contrasting irrigating regimes over the two-year experiment and search for the candidate genes encompassed in the mapped QTLs.ResultsWe constructed a high-density genetic linkage map with 10,172 markers of apple, using single nucleotide polymorphism (SNP) markers obtained through restriction site-associated DNA sequencing (RADseq) and a final segregating population of 350 seedlings from the cross of Honeycrisp and Qinguan. In total, 33 QTLs were identified for carbon isotope composition in apple under both well-watered and drought-stressed conditions. Three QTLs were stable over 2 years under drought stress on linkage groups LG8, LG15 and LG16, as validated by Kompetitive Allele-Specific PCR (KASP) assays. In those validated QTLs, 258 genes were screened according to their Gene Ontology functional annotations. Among them, 28 genes were identified, which exhibited significant responses to drought stress in ‘Honeycrisp’ and/or ‘Qinguan’. These genes are involved in signaling, photosynthesis, response to stresses, carbohydrate metabolism, protein metabolism and modification, hormone metabolism and transport, transport, respiration, transcriptional regulation, and development regulation. They, especially those for photoprotection and relevant signal transduction, are potential candidate genes connected with WUE regulation in drought-stressed apple.ConclusionsWe detected three stable QTLs for carbon isotope composition in apple under drought stress over 2 years, and validated them by KASP assay. Twenty-eight candidate genes encompassed in these QTLs were identified. These stable genetic loci and series of genes provided here serve as a foundation for further studies on marker-assisted selection of high WUE and regulatory mechanism of WUE in apple exposed to drought conditions, respectively.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1308-3) contains supplementary material, which is available to authorized users.

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“…2009). A recent study developed on an apple progeny shows that stomatal conductance strongly decreases with increased vapour pressure deficit (VPD) with high broad‐sense heritability for this trait (Regnard et al . 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic determinism of anatomical and hydraulic traits within an apple progeny

Lauri

Gorza

Cochard

et al. 2011

Plant Cell & Environment

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The apple tree is known to have an isohydric behaviour, maintaining rather constant leaf water potential in soil with low water status and/or under high evaporative demand. However, little is known on the xylem water transport from roots to leaves from the two perspectives of efficiency and safety, and on its genetic variability. We analysed 16 traits related to hydraulic efficiency and safety, and anatomical traits in apple stems, and the relationships between them. Most variables were found heritable, and we investigated the determinism underlying their genetic control through a quantitative trait loci (QTL) analysis on 90 genotypes from the same progeny. Principal component analysis (PCA) revealed that all traits related to efficiency, whether hydraulic conductivity, vessel number and area or wood area, were included in the first PC, whereas the second PC included the safety variables, thus confirming the absence of tradeoff between these two sets of traits. Our results demonstrated that clustered variables were characterized by common genomic regions. Together with previous results on the same progeny, our study substantiated that hydraulic efficiency traits co-localized with traits identified for tree growth and fruit production.

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QTL Analysis for Leaf Gas Exchange in an Apple Progeny Grown Under Atmospheric Constraints

Cited by 11 publications

References 0 publications

Multispectral airborne imagery in the field reveals genetic determinisms of morphological and transpiration traits of an apple tree hybrid population in response to water deficit

Multispectral airborne imagery in the field reveals genetic determinisms of morphological and transpiration traits of an apple tree hybrid population in response to water deficit

Mapping QTLs for water-use efficiency reveals the potential candidate genes involved in regulating the trait in apple under drought stress

Genetic determinism of anatomical and hydraulic traits within an apple progeny

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