qPCR Detection of Early Life History Stage<i>Chrysaora quinquecirrha</i>(Sea Nettles) in Barnegat Bay, New Jersey

Gaynor, John J.; Bologna, Paul A.X.; Restaino, Dena J.; Barry, Christie L.

doi:10.2112/si78-014.1

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…This technique has been used to detect rare (Jerde et al., 2011; Keskin et al., 2016; Simpfendorfer et al., 2016), invasive (Robson et al., 2016), and sporadically distributed species, including scyphozoan jellyfish. For example, Minamoto et al., (2017) used an eDNA approach to detect the presence of medusae of the Japanese sea nettle ( Chrysaora pacifica ) in Mazizuru Bay, Kyoto, while Gaynor et al., (2017) looked at the applicability of eDNA to detect early life stages of Chrysaora quinquecirrha in Barnegat Bay, New Jersey. However, to date, there has been no published works on the use of an eDNA approach to detect occurrence of venomous cubozoans and the focus has only been on ephyrae and medusa, not the benthic polyps.…”

Section: Introductionmentioning

confidence: 99%

Validation of eDNA as a viable method of detection for dangerous cubozoan jellyfish

et al. 2021

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Stings from certain species of cubozoan jellyfish are dangerous to humans and their seasonal presence in tropical marine waters poses a significant risk to coastal communities. The detection of cubozoans is difficult due to high spatial and temporal variation in their occurrence and abundance. Environmental DNA (eDNA) has the potential to detect rare species and therefore offers potential to detect cubozoans, not only pelagic medusae, but presence of cryptic polyp life stages. The objective of this study was to validate the use of eDNA as a viable detection method for four cubozoan species (Chironex fleckeri, Copula sivickisi, Carybdea xaymacana, and Carukia barnesi). Species-specific primers were developed for each of these four cubozoans and an eDNA approach validated utilizing both laboratory and field trials. Laboratory DNA degradation experiments demonstrated that C. sivickisi DNA degraded quickly but could still be detected in sea water for up to 9 days post-jellyfish removal.Positive detection was found for C. fleckeri, C. xaymacana, and C. sivickisi medusae in the waters surrounding Magnetic Island, Queensland, in the Austral spring/summer (September-January). Based on visual surveys, there was a poor relationship between concentration of eDNA and abundance of jellyfish. Positive eDNA amplification was also found from water sampled near the substratum when C. sivickisi medusae were out of season and absent. This suggests the eDNA analysis was likely detecting C. sivickisi polyps located within the substratum. Consequently, eDNA is an effective tool to detect both the medusae and likely polyps of cubozoans. This approach provides the means to reduce the risk of envenomation to swimmers and enhance our knowledge of cubozoan ecology.

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Section: Introductionmentioning

confidence: 99%

Validation of eDNA as a viable method of detection for dangerous cubozoan jellyfish

et al. 2021

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“…As a new marine ecological survey tool, eDNA-based techniques are non-invasive, environmentally friendly, and accurate, and they are expected to play an essential role in optimizing ecological studies of jellyfish [ 42 – 44 ]. Recent studies have highlighted the great potential of eDNA-based techniques in biodiversity investigation, spatial distribution detection, and early life history stage monitoring of jellyfish [ 45 – 48 ]. Metabarcoding, as one of the major eDNA-based techniques, has obvious advantages in providing broad-scale distribution data for multiple species from a single analysis and detecting unknown biodiversity previously not recorded, including “unexpected” invasive or non-native species [ 21 , 23 , 28 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Biodiversity and distribution patterns of blooming jellyfish in the Bohai Sea revealed by eDNA metabarcoding

Ye,

Peng,

et al. 2024

BMC Ecol Evo

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Background The mass occurrence of scyphozoan jellyfish severely affects marine ecosystems and coastal economies, and the study of blooming jellyfish population dynamics has emerged in response. However, traditional ecological survey methods required for such research have difficulties in detecting cryptic life stages and surveying population dynamics owing to high spatiotemporal variations in their occurrence. The environmental DNA (eDNA) technique is an effective tool for overcoming these limitations. Results In this study, we investigated the biodiversity and spatial distribution characteristics of blooming jellyfish in the Bohai Sea of China using an eDNA metabarcoding approach, which covered the surface, middle, and bottom seawater layers, and sediments. Six jellyfish taxa were identified, of which Aurelia coerulea, Nemopilema nomurai, and Cyanea nozakii were the most dominant. These three blooming jellyfish presented a marked vertical distribution pattern in the offshore regions. A. coerulea was mainly distributed in the surface layer, whereas C. nozakii and N. nomurai showed a upper-middle and middle-bottom aggregation, respectively. Horizontally, A. coerulea and C. nozakii were more abundant in the inshore regions, whereas N. nomurai was mainly distributed offshore. Spearman’s correlation analysis revealed a strong correlation between the eDNA of the three dominant blooming jellyfish species and temperature, salinity, and nutrients. Conclusions Our study confirms the applicability of the eDNA approach to both biodiverstiy evaluation of blooming jellyfish and investigating their spatial distribution, and it can be used as a supplementary tool to traditional survey methods.

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“…Recently, a study compared the zooplankton taxa recovered with nets and with eDNA of filtered water samples, and demonstrated eDNA to be particularly suitable for revealing gelatinous diversity (Govindarajan et al, 2021). However, eDNA studies focusing on gelatinous zooplankton still remain relatively scarce (Ames et al, 2021;Gaynor et al, 2017;Minamoto et al, 2017;Takasu et al, 2019).…”

Section: The Elusive Rare and Endangered: Edna As A Non-invasive Methodsmentioning

confidence: 99%

Environmental Dna in an Ocean of Change: Status, Challenges and Prospects

Havermans¹,

Dischereit²,

Pantiukhin³

et al. 2022

ACMAR

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Environmental DNA (eDNA) studies have burgeoned over the last two decades and the application of eDNA has increased exponentially since 2010, albeit at a slower pace in the marine system. We provide a literature overview on marine metazoan eDNA studies and assess recent achievements in answering questions related to species distributions, biodiversity and biomass. We investigate which are the better studied taxonomic groups, geographic regions and the genetic markers used. We evaluate the use of eDNA for addressing ecological and environmental issues through food web, ecotoxicological, surveillance and management studies. Based on this state of the art, we highlight exciting prospects of eDNA for marine time series, population genetic studies, the use of natural sampler DNA, and eDNA data for building trophic networks and ecosystem models. We discuss the current limitations, in terms of marker choice and incompleteness of reference databases. We also present recent advances using experiments and modeling to better understand persistence, decay and dispersal of eDNA in coastal and oceanic systems. Finally, we explore promising avenues for marine eDNA research, including autonomous or passive eDNA sampling, as well as the combined applications of eDNA with different surveillance methods and further molecular advances. Keywords: environmental DNA, DNA metabarcoding, marine metazoa, biodiversity, population genetics, natural sampler DNA, diet analysis.

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qPCR Detection of Early Life History StageChrysaora quinquecirrha(Sea Nettles) in Barnegat Bay, New Jersey

Cited by 11 publications

References 43 publications

Validation of eDNA as a viable method of detection for dangerous cubozoan jellyfish

Validation of eDNA as a viable method of detection for dangerous cubozoan jellyfish

Biodiversity and distribution patterns of blooming jellyfish in the Bohai Sea revealed by eDNA metabarcoding

Environmental Dna in an Ocean of Change: Status, Challenges and Prospects

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