QiShenYiQi Pills® prevents cardiac ischemia–reperfusion injury via energy modulation

Lin, Se-Qi; Wei, Xiao‐Hong; Huang, Ping; Liu, Yuying; Zhao, Na; Li, Quan; Pan, Chun‐Shui; Hu, Bai‐He; Chang, Xin; Fan, Jing‐Yu; Yang, Xiaoyuan; Wang, Chaun-She; Liu, Hongning; Han, Jing‐Yan

doi:10.1016/j.ijcard.2012.10.042

Cited by 53 publications

(58 citation statements)

References 14 publications

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“…The magnitude of MBF was graded by different colors, with blue to red denoting low to high. Results were presented as percent of the baseline MBF13.…”

Section: Methodsmentioning

confidence: 99%

“…The myocardial IA, AAR and LV in each section was determined by Image-Pro Plus 6.0 (Media Cybernetic, Bethesda, MD, USA), and the ratios AAR/LV (%) and infarct area/AAR (%) were calculated. The values from five sections were averaged and used to score the degree of myocardial infarction13.…”

Section: Methodsmentioning

confidence: 99%

“…The nuclei were labeled with Hoechest33342. Five fields were selected from the surrounding infarction areas of the LV for each section at 40x magnification of objective, and observed with a Laser Scanning Confocal Microscope (TCS SP5; Leica, Mannheim, Germany)13.…”

Section: Methodsmentioning

confidence: 99%

“…Myocardial tissue was collected at one third above the apex cordis from the surrounding infarct region of left ventricle and cut into 1 mm 3 blocks. The tissue blocks were fixed overnight at 4 °C with 3% glutaraldehyde, washed 3 times with 0.1 mol/L phosphate-buffered solution, and then post-fixed with 1% osmium tetraoxide for 2 h. The ultrathin sections were prepared as routine, observed and photographed with a transmission electron microscope (JEM 1230, JEOL, Tokyo, Japan)13.…”

Section: Methodsmentioning

confidence: 99%

“…However, the underlying mechanisms are not fully understood. QiShenYiQi Pills ® (a compound Chinese medicine containing Rb1 as a major component) has proven able to regulate energy metabolism deficiency and expression of ATP 5D in cardiac I/R injury1213. Our previous study reported that notoginsenoside R1 (NR1) ameliorated I/R induced myocardial injury by regulating levels of ROCK1 and ATP5D, implying a likely link between RhoA signaling pathway and energy metabolism regulation4.…”

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confidence: 99%

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Ginsenoside Rb1 protects against ischemia/reperfusion-induced myocardial injury via energy metabolism regulation mediated by RhoA signaling pathway

Cui

Pan

et al. 2017

Sci Rep

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Cardiac ischemia and reperfusion (I/R) injury remains a challenge for clinicians. Ginsenoside Rb1 (Rb1) has been reported to have the ability to attenuate I/R injury, but its effect on energy metabolism during cardiac I/R and the underlying mechanism remain unknown. In this study, we detected the effect of Rb1 on rat myocardial blood flow, myocardial infarct size, cardiac function, velocity of venule red blood cell, myocardial structure and apoptosis, energy metabolism and change in RhoA signaling pathway during cardiac I/R injury. In addition, the binding affinity of RhoA to Rb1 was detected using surface plasmon resonance (SPR). Results showed that Rb1 treatment at 5 mg/kg/h protected all the cardiac injuries induced by I/R, including damaged myocardial structure, decrease in myocardial blood flow, impaired heart function and microcirculation, cardiomyocyte apoptosis, myocardial infarction and release of myocardial cTnI. Rb1 also inhibited the activation of RhoA signaling pathway and restored the production of ATP during cardiac I/R. Moreover, SPR assay showed that Rb1 was able to bind to RhoA in a dose-dependent manner. These results indicate that Rb1 may prevent I/R-induced cardiac injury by regulation of RhoA signaling pathway, and may serve as a potential regime to improve percutaneous coronary intervention outcome.

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“…The magnitude of MBF was graded by different colors, with blue to red denoting low to high. Results were presented as percent of the baseline MBF13.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Ginsenoside Rb1 protects against ischemia/reperfusion-induced myocardial injury via energy metabolism regulation mediated by RhoA signaling pathway

Cui

Pan

et al. 2017

Sci Rep

Self Cite

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Screening bioactive quality control markers of QiShenYiQi dripping pills based on the relationship between the ultra‐high performance liquid chromatography fingerprint and vascular protective activity

Zhuo

Peng

Zhao

et al. 2017

J of Separation Science

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Traditional Chinese medicine consists of complex phytochemical constituents. Selecting appropriate analytical markers of traditional Chinese medicine is a critical step in quality control. Currently, the combination of fingerprinting and efficacy evaluation is considered as a useful method for screening active ingredients in complex mixtures. This study was designed to develop an orthogonal partial least squares model for screening bioactive quality control markers of QishenYiqi dripping pills based on the fingerprint-efficacy relationship. First, the chemical fingerprints of 49 batches of QishenYiqi dripping pill samples were established by ultra-high performance liquid chromatography coupled with a photodiode array detector. Second, ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry was exploited to systematically investigate the 36 copossessing fingerprint components in QishenYiqi dripping pills. The vascular protective activity of QishenYiqi dripping pills was determined by using a cell counting kit-8 assay. Finally, fingerprint-efficacy relationship was established by orthogonal partial least squares model. The results indicated that ten components exhibited strong correlation with vascular protective activity, and these were preliminarily screened as quality control markers. The present study provided a novel idea for the study of the pharmacodynamic material basis and quality evaluation of QishenYiqi dripping pills.

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Effects of Qishen Yiqi Dripping Pills (芪参益气滴丸) in Reducing Myocardial Injury and Preserving Microvascular Function in Patients Undergoing Elective Percutaneous Coronary Intervention: A Pilot Randomized Study

Xie

Jiang

et al. 2017

Chin. J. Integr. Med.

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QiShenYiQi Pills® prevents cardiac ischemia–reperfusion injury via energy modulation

Cited by 53 publications

References 14 publications

Ginsenoside Rb1 protects against ischemia/reperfusion-induced myocardial injury via energy metabolism regulation mediated by RhoA signaling pathway

Ginsenoside Rb1 protects against ischemia/reperfusion-induced myocardial injury via energy metabolism regulation mediated by RhoA signaling pathway

Screening bioactive quality control markers of QiShenYiQi dripping pills based on the relationship between the ultra‐high performance liquid chromatography fingerprint and vascular protective activity

Effects of Qishen Yiqi Dripping Pills (芪参益气滴丸) in Reducing Myocardial Injury and Preserving Microvascular Function in Patients Undergoing Elective Percutaneous Coronary Intervention: A Pilot Randomized Study

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