QiShen YiQi and its components attenuate acute thromboembolic stroke and carotid thrombosis by inhibition of CD62P/PSGL-1-mediated platelet-leukocyte aggregate formation

Yu, Mingxing; Xiao, Guangxu; Han, Linhong; Li, Peng; Wang, Huanyi; He, Shuang; Lyu, Ming; Zhu, Yan

doi:10.1016/j.biopha.2023.114323

Cited by 4 publications

(4 citation statements)

References 81 publications

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“…P-selectin is stored in the Weibel-Palade bodies of endothelial cells and the α-granules of platelets ( Ling et al, 2023 ). In the event of a stroke, P-selectin is rapidly translocated to the cell surface, where it plays a key role in the initial tethering and rolling of leukocytes along the endothelium by interacting with its ligands (like PSGL-1) on leukocytes ( Yu et al, 2023 ). E-selectin is distinctly inducible and expressed on endothelial cells in response to inflammatory cytokines, particularly TNF-α and IL-1 ( Takeda et al, 2002 ).…”

Section: Inflammatory Markersmentioning

confidence: 99%

Emerging diagnostic markers and therapeutic targets in post-stroke hemorrhagic transformation and brain edema

Yao,

Liu,

et al. 2023

Front. Mol. Neurosci.

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Stroke is a devastating condition that can lead to significant morbidity and mortality. The aftermath of a stroke, particularly hemorrhagic transformation (HT) and brain edema, can significantly impact the prognosis of patients. Early detection and effective management of these complications are crucial for improving outcomes in stroke patients. This review highlights the emerging diagnostic markers and therapeutic targets including claudin, occludin, zonula occluden, s100β, albumin, MMP-9, MMP-2, MMP-12, IL-1β, TNF-α, IL-6, IFN-γ, TGF-β, IL-10, IL-4, IL-13, MCP-1/CCL2, CXCL2, CXCL8, CXCL12, CCL5, CX3CL1, ICAM-1, VCAM-1, P-selectin, E-selectin, PECAM-1/CD31, JAMs, HMGB1, vWF, VEGF, ROS, NAC, and AQP4. The clinical significance and implications of these biomarkers were also discussed.

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Section: Inflammatory Markersmentioning

confidence: 99%

Emerging diagnostic markers and therapeutic targets in post-stroke hemorrhagic transformation and brain edema

Yao,

Liu,

et al. 2023

Front. Mol. Neurosci.

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“…Therefore, domestic and foreign scholars always use certain techniques and methods to quantify the active ingredients in the analysis of TCM, which aims to achieve the overall evaluation of TCM—Chinese herbal decoction pieces—intermediates—TCM preparations. In the study of Dai et al (2021) and Yu et al (2023), the ingredients of Qishen Yiqi dripping pills, tanshinone, catechin, salvianolic acid B, rosmarinic acid, and cryptotanshinone have various pharmacological activities, especially in reducing myocardial oxygen consumption and stabilizing heart rate. Generally, the HPLC method is used to analyze its content.…”

Section: Introductionmentioning

confidence: 99%

Determination of Four Chemical Components in Qishen Yiqi Dripping Pills by UPLC-MS/MS Method

Lili,

Honglian,

Yanxia

et al. 2023

Pharmacognosy Magazine

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Background In this study, the ultrahigh performance liquid chromatography mass spectrometry/mass spectrometry (UPLC-MS/MS) method was established to test the contents of protocatechuic acid (C7H6O4), salvianolic acid B (C36H30O16), rosmarinic acid (C19H14O3), and cryptotanshinone (C19H20O3) in Qishen Yiqi dripping pills. Materials and Methods Methanol was chosen as the solvent according to a certain material–liquid ratio as a solution, and the ultrasonic extraction method was used to prepare the sample solution. UPLC-MS/MS was used to complete a quantitative analysis of the contents of Qishen Yiqi dripping pills. Then, the chromatographic conditions were shown as follows. The stationary phase was Agilent ZORBAX SB-C18 (2.1 mm × 100 mm, 1.8 µm), and the mobile phase was acetonitrile–0.1% formic acid water. The column temperature was 40℃, the injection volume was 3 µL, and the flow rate was 0.2 mL⋅min–1. Finally, the determination method was built to test four chemical constituents under the multiple reaction monitoring (MRM) analysis mode. Results The retention times of protocatechuic acid, salvianolic acid B, rosmarinic acid, and cryptotanshinone were 1.26, 1.32, 1.35, and 6.76 min, respectively. In addition, the concentration ranges of four compounds were 0.25–2.0 µg⋅mL–1, 10.0–50.0 µg⋅mL–1, 5.0–50.0 µg⋅mL–1, and 1.0–10.0 µg⋅mL–1, respectively. And the standard curve showed a good linear relationship in a reasonable range ( r2 ≥ 0.9950). The average recovery rate was from 96% to 124%. The contents of protocatechuic acid, salvianolic acid B, rosmarinic acid, and cryptotanshinone were 0.12%–0.022%, 17%–8.4%, and 6.5%–8.6% in seven batches of Qishen Yiqi dripping pills, which were in the range of 23%–31%. The instrument ran stably, and the method had good accuracy and repeatability (relative standard deviation [RSD] ≤ 3%). Additionally, the test result was stable within 10 h. Conclusion The contents of the four constituents were different among different batches. The quantitative method was established to determine the four chemical components in Qishen Yiqi dripping pills, which could provide a reference for quality evaluation in vivo.

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“…PSGL-1, one of the best-characterized selectin ligands, is mainly expressed in leukocytes ( 12 ). Leukocyte platelet aggregation formed by adhesion between activated platelet and mature leukocyte is mediated by CD62P and PSGL-1 ( 13 – 15 ). The formation of leukocyte platelet aggregation (LPA) is necessary for effective migration to the infection site ( 16 ), which might be induced by L. major surface membrane components.…”

Section: Introductionmentioning

confidence: 99%

“…By interaction with macrophage cells, monocytes, neutrophils, lymphocytes, and the endothelium, platelets are therefore important executors during inflammatory and immune responses (8,9). The membrane glycoprotein CD62P, a member of the selectin family, is expressed on activated platelets (10) and is redistributed from the secretory a-granules (11). PSGL-1, one of the best-characterized selectin ligands, is mainly expressed in leukocytes (12).…”

Section: Introductionmentioning

confidence: 99%

Leishmania major-derived lipophosphoglycan influences the host’s early immune response by inducing platelet activation and DKK1 production via TLR1/2

Ihedioha,

Sivakoses,

Beverley

et al. 2023

Front. Immunol.

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BackgroundPlatelets are rapidly deployed to infection sites and respond to pathogenic molecules via pattern recognition receptors (TLR, NLRP). Dickkopf1 (DKK1) is a quintessential Wnt antagonist produced by a variety of cell types including platelets, endothelial cells, and is known to modulate pro-inflammatory responses in infectious diseases and cancer. Moreover, DKK1 is critical for forming leukocyte-platelet aggregates and induction of type 2 cell-mediated immune responses. Our previous publication showed activated platelets release DKK1 following Leishmania major recognition.ResultsHere we probed the role of the key surface virulence glycoconjugate lipophosphoglycan (LPG), on DKK1 production using null mutants deficient in LPG synthesis (Δlpg1- and Δlpg2-). Leishmania-induced DKK1 production was reduced to control levels in the absence of LPG in both mutants and was restored upon re-expression of the cognate LPG1 or LPG2 genes. Furthermore, the formation of leukocyte-platelet aggregates was dependent on LPG. LPG mediated platelet activation and DKK1 production occurs through TLR1/2.ConclusionThus, LPG is a key virulence factor that induces DKK1 production from activated platelets, and the circulating DKK1 promotes Th2 cell polarization. This suggests that LPG-activated platelets can drive innate and adaptive immune responses to Leishmania infection.

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QiShen YiQi and its components attenuate acute thromboembolic stroke and carotid thrombosis by inhibition of CD62P/PSGL-1-mediated platelet-leukocyte aggregate formation

Cited by 4 publications

References 81 publications

Emerging diagnostic markers and therapeutic targets in post-stroke hemorrhagic transformation and brain edema

Emerging diagnostic markers and therapeutic targets in post-stroke hemorrhagic transformation and brain edema

Determination of Four Chemical Components in Qishen Yiqi Dripping Pills by UPLC-MS/MS Method

Leishmania major-derived lipophosphoglycan influences the host’s early immune response by inducing platelet activation and DKK1 production via TLR1/2

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