Qipengyuania sediminis gen. nov., sp. nov., a member of the family Erythrobacteraceae isolated from subterrestrial sediment

Abstract

Qipengyuania sediminis gen. nov., sp. nov., a member of the family Erythrobacteraceae isolated from subterrestrial sediment Oil and Gas Survey, China Geological Survey, Beijing 10029, PR China A Gram-reaction-negative, non-motile, facultatively aerobic bacterium, designated strain M1 T , was isolated from a subterrestrial sediment sample of Qiangtang Basin in Qinghai-Tibetan plateau, China. The strain formed rough yellow colonies on R2A plates. Cells were oval or short rod-shaped, catalase-positive and oxidase-negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate belonged to the family Erythrobacteraceae and showed 96.2-96.4 % 16S rRNA gene sequence similarities to its closest relatives. Chemotaxonomic analysis revealed ubiquinone-10 (Q10) as the dominant respiratory quinone of strain M1 T and C 17 : 1 v6c (44.2 %) and C 18 : 1 v7c (13.7 %) as the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid, three unidentified glycolipids, one unidentified phosphoglycolipid and one unidentified lipid. The DNA G+C content of strain M1 T was 73.7 mol%. On the basis of phenotypic, phylogenetic and genotypic data presented in this study, strain M1 T represents a novel species of a new genus in the family Erythrobacteraceae, for which the name Qipengyuania sediminis gen. nov., sp. nov. is proposed. The type strain of the type species is M1 T (5CGMCC 1.12928 T 5JCM 30182 T ).

Genomic-based taxonomic classification of the family Erythrobacteraceae

“…The genera Croceibacterium and Croceicoccus formed two independent clades, and they did not belong to monophyletic clades which could be separated from other genera. Thus, 16S rRNA gene sequences did not confirm monophyletic relationships within the genera of the family [2, 4, 30, 37, 38]. Only 19 nodes accounting for 26.0 % exhibited bootstrap values higher than 70 %, indicating that this phylogenetic tree was not reliable enough to correctly reveal the taxonomic status of the genera of the family.…”

“…The family Erythrobacteraceae , belonging to the order Sphingomonadales , class Alphaproteobacteria [1], is distributed globally, inhabiting various environments including subterrestrial, lake, intertidal areas, mangrove, coastal and deep-sea sediments [2–10], soil [11–13], desert sands [14, 15], a stadium seat [16], seawater [17–19], estuary water [20–22], fresh water [23, 24], hot springs [25–27], air [28] as well as plants and animals [29–36] (Table S1, available in the online version of this article). The members of the family Erythrobacteraceae are Gram-stain-negative, rod or pleomorphic coccoid-shaped, pink-, red-, orange- or yellow-pigmented, and aerobic chemoorganotrophs [1].…”

“…The majority require NaCl for growth [1]. Ubiquinone-10 (Q-10) is the major respiratory quinone [1, 2, 30].…”

“…were later proposed by Kwon et al . [38], Liu et al [30], Xu et al [4] and Feng et al [2], respectively, based on 16S rRNA gene phylogeny [2, 4, 30, 38]. At the time of writing (September 2019), the genera Altererythrobacter , Croceibacterium , Croceicoccus , Erythrobacter , Erythromicrobium , Porphyrobacter and Qipengyuania consist of 41, two, four, 23, one, eight and one species, respectively [9, 10, 17, 19, 22, 30, 35, 39–43].…”

Pseudopontixanthobacter vadosimaris gen. nov., sp. nov., isolated from shallow sea near Kueishan Island

“…[1] based on 16S rRNA gene phylogeny in 2005, and only three genera ( Erythrobacter , Erythromicrobium and Porphyrobacter ) were included. The genera Altererythrobacter [2], Croceibacterium [3], Croceicoccus [4] and Qipenguania [5] were proposed later. Before writing this study (May 2020), 16S rRNA gene phylogenetic analysis played a large role in determining taxa within the family.…”